重离子与X射线辐照对人舌鳞状细胞癌Tca8113细胞株CXCR4表达的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 789-.

重离子与X射线辐照对人舌鳞状细胞癌Tca8113细胞株CXCR4表达的影响

李馨^1,2,李春青³,刘兰¹,毛玮刚¹,李涛³,冯正虎^3*,杨兰^1*

1. 兰州大学第二医院口腔颌面外科甘肃兰州 730030;
2. 兰州大学口腔医学院;
3. 西北民族大学口腔医学国家民委重点实验室

收稿日期:2015-02-02 出版日期:2015-08-28 发布日期:2016-05-04
通讯作者: 杨兰,电话:13609303257;冯正虎,电话:13919956823
作者简介:李馨(1988~ ),女,河南人,住院医师,硕士,主要从事口腔颌面外科临床及科研工作。
基金资助:
国家自然科学基金(编号:81260403);甘肃省科技支撑计划(编号:1304FKCA092)

Effects of Heavy Ion Beams and X-ray on CXCR4 Expression of Human Tongue Squamous Cell Carcinoma Tca8113 Cell Line

LI Xin, LI Chun-qing, LIU Lan, et al

Department of Oral and Maxillofical Surgery, Second Hospital, Lanzhou University, Lanzhou 730030

Received:2015-02-02 Online:2015-08-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:以CXCR4mRNA的表达量为指标探讨不同辐射方式对Tca8113细胞株细胞侵袭能力的影响。方法:采用不同剂量的重离子束与X-ray对细胞进行辐射,采用荧光实时定量PCR法观察细胞中CXCR4mRNA表达量的变化。结果:与空白对照组比较,在相同辐射剂量下,X线辐照组细胞中CXCR4的表达量在8 h时较低,于12 h达到高峰,该峰值在1Gy、4Gy组较为显著(P<0.05),在2Gy组则差异不明显;而重离子辐照组细胞中CXCR4的mRNA表达水平在辐照后12 h内均明显降低(P<0.05)。两者在24 h的表达基本恢复至空白对照组水平。在相同时间点时,重离子组CXCR4的表达量与X线组比较则有显著降低(P<0.01)。结论:与常规X线相比,重离子对体外培养的人舌鳞癌细胞中CXCR4的表达的抑制能力更强,提示重离子抑制舌鳞癌细胞侵袭的能力优于常规X射线。

关键词: 重离子, X射线, 舌鳞癌, CXCR4, 肿瘤侵袭

Abstract: Objective: To study the effects of heavy ion beams and X-ray on the expression of chemokine receptor CXCR4 in Tca8113 cell line, and explore the underlying mechanism. Methods: The mRNA express level of CXCR4 was detected by real-time PCR after Tca8113 cells were treated. Results: Compared with the control group, the expression of CXCR4 in the X-ray group got to the lowest level at 8 h, and reached the peak value at 12 h (P<0.05) after radiation. In addition, the changes in the 1 Gy and 4 Gy groups were more obvious (P<0.05), but not significant in the 2Gy group. The expression of CXCR4 was significantly down-regulated at 12 h after heavy ion beams treatment. Meanwhile, the expression of CXCR4 in the 1 Gy and 4 Gy groups returned to the control level at 24 h after radiation. Compared with X-ray group, CXCR4 expression was obviously lower in the heavy ion beams group (P<0.01). Conclusion: Heavy ion beams may be a better choice to inhibit the invasion of Tca8113 cell line in vitro.

Key words: Heavy ion beams, X-ray, Tongue squamous cell carcinoma, CXCR4, Tumor invasion

中图分类号:

R739.8

李馨,李春青,刘兰,毛玮刚,李涛,冯正虎,杨兰. 重离子与X射线辐照对人舌鳞状细胞癌Tca8113细胞株CXCR4表达的影响[J]. 口腔医学研究, 2015, 31(8): 789-.

LI Xin, LI Chun-qing, LIU Lan, et al. Effects of Heavy Ion Beams and X-ray on CXCR4 Expression of Human Tongue Squamous Cell Carcinoma Tca8113 Cell Line[J]. Journal of Oral Science Research, 2015, 31(8): 789-.

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