口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (9): 863-866.

• 基础研究论著 • 上一篇    下一篇

牙龈卟啉单胞菌感染MG63细胞对细胞周期的影响

潘春玲1,赵海礁1,谭丽思1,潘亚萍1*,钟鸣2   

  1. 1. 中国医科大学口腔医学院牙周科、辽宁省口腔医学研究所牙周病研究室、辽宁省口腔疾病转化医学研究所 辽宁 沈阳 110002;
    2. 中国医科大学口腔医学院中心实验室
  • 收稿日期:2015-03-16 出版日期:2015-09-28 发布日期:2016-05-04
  • 通讯作者: 潘亚萍,E-mail: yppan@mail.cmu.edu.cn
  • 作者简介:潘春玲(1975~ ),女,副教授,博士,主要从事口腔微生物学的研究工作。
  • 基金资助:
    国家自然科学基金资助项目(编号:81070834和81271153),同时该项目受到国家临床重点专科的支持

Effects of Porphyromonas gingivalis Infection on the Cell Cycle of MG63 Cells

PAN Chun-ling1, ZHAO Hai-jiao1, TAN li-si1, PAN Ya-ping1*, ZHONG Ming2   

  1. 1. Department of Periodontics, School of Stomatology, China Medical University; Laboratory of Periodontics, Liaoning Institute of Dental Research, Shenyang 110002, China. 2. Central Laboratory, School of Stomatology, China Medical University, Shenyang 110002, China
  • Received:2015-03-16 Online:2015-09-28 Published:2016-05-04

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摘要： 目的:通过体外建立牙龈卟啉单胞菌(P. gingivalis)感染MG63细胞模型,探讨P. gingivalis ATCC 33277菌株感染MG63细胞对细胞周期的影响及发生机制。方法:P. gingivalis ATCC 33277菌株感染MG63细胞,MTT法检测细胞的增殖,流式细胞仪检测细胞周期的分布,实时定量PCR和蛋白印记方法检测Cyclin D、Cyclin E的表达。结果:MOI为100∶1,P. gingivalis ATCC 33277菌株感染MG63细胞,能够抑制细胞增殖,同时导致细胞在G1期发生停滞,Cyclin D、Cyclin E基因和蛋白在24 h、48 h均有明显下降,与对照组相比有统计学差异。结论:牙龈卟啉单胞菌感染MG63细胞Cyclin D、Cyclin E基因和蛋白表达下调,抑制细胞的增殖,导致细胞停滞在G1期。

关键词: 牙周炎, 牙龈卟啉单胞菌, 细胞周期

Abstract: Objective: To investigate the effects and regulatory mechanism on the cell cycle of MG63 cells after infected with P. gingivalis in vitro. Methods: The cell proliferation activity and cell cycle distribution of MG63 cells were determined after infected with P. gingivalis ATCC 33277. The protein and mRNA of Cyclin D and Cyclin E were determined with real-time quantitative RT-PCR and Western immunoblot in MG63 cells after infected with P. gingivalis ATCC 33277. Results: The proliferation rate and the proportion of G1 phase in MG63 cells infected with P. gingivalis were significantly different from those of the control at 24h and 48h. The mRNA and protein expression levels of Cyclin D and E in the infected cells significantly decreased at 24 and 48h. Conclusion: MG63 cells infected with P. gingivalis reduce cell proliferation and arrest in G1 phase with low expression of mRNA and protein of Cyclin D and Cyclin E.

Key words: Periodontitis, Porphyromonas gingivalis, Cell cycle

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