口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (9): 949-952.DOI: 10.13701/j.cnki.kqyxyj.2016.09.014

• 临床研究论著 • 上一篇    下一篇

miR-146a在口腔扁平苔藓患者外周血及组织中表达及与IRAK1和TRAF6表达的相关性

周辉1*,唐欣轶1,郭燕2   

  1. 1. 武汉市第三医院口腔科 湖北 武汉 430060;
    2. 中国医科大学第四医院 辽宁 沈阳 110032
  • 收稿日期:2015-12-21 出版日期:2016-09-26 发布日期:2016-09-26
  • 通讯作者: 周辉,电话:18202728763
  • 作者简介:周辉(1982~ ),男,湖北武汉人,硕士,主治医师,主要从事口腔临床治疗工作。
  • 基金资助:
    辽宁省自然科学基金(编号:2013021094)

Expressions of miR-146a in Patients with Orallichen Planus and Its Correlation with IRAK1 and TRAF6

ZHOU Hui1, TANG Xin-yi1, GUO Yan2   

  1. 1. Department of Stomatology, The Third Hospital of Wuhan. Wuhan 430060, China;
    2. Department of Stomatology, The Fourth Hospital of China Medical University, Shenyang 110032, China
  • Received:2015-12-21 Online:2016-09-26 Published:2016-09-26

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摘要： 目的:探讨miR-146a在口腔扁平苔藓患者外周血及组织中表达及意义。方法:选取93例口腔扁平苔藓患者(OLP),包括非糜烂型53例和糜烂型40例,同期,选取健康者42例,利用反转录实时定量PCR(RT-qPCR)技术检测OLP患者外周血及组织中miR-146a、IRAK1和TRAF6表达,Pearson相关分析分析miR-146a与IRAK1和TRAF6表达相关性。结果:miR-146a在OLP患者血浆和组织中相对表达量均高于健康者,IRAK1 mRNA和TRAF6 mRNA在OLP患者血浆和组织中相对表达量均低于健康者,差异均有统计学意义(P<0.05);糜烂型OLP患者血浆和组织中miR-146a相对表达量均高于非糜烂型OLP患者,而IRAK1 mRNA和TRAF6 mRNA相对表达量均低于非糜烂型OLP患者,差异均有统计学意义(P<0.05);Pearson相关分析显示,OLP患者血浆和组织中miR-146a相对表达量均与IRAK1 mRNA和TRAF6 mRNA相对表达量呈负相关(r=-0.358、-0.345和-0.312、-0.379,P<0.05)。结论:miR-146a在OLP患者外周血及组织中呈高表达,且与病损程度有关,推测可能通过抑制IRAK1和TRAF6而发挥负调控作用参与OLP免疫炎性反应。

关键词: 口腔扁平苔藓, miR-146a, 外周血, 组织, 表达

Abstract: Objective: To investigate the expression of miR-146a in peripheral blood and tissues of patients with oral lichen planus. Methods: Ninety-three patients with orallichen planus (OLP) were selected, including 53 cases of non-erosive type and 40 cases of erosive type. The expressions of miR-146a, IRAK1 and TRAF6 in peripheral blood and tissues were detected using real-time quantitative reverse transcription PCR (RT-qPCR). The correlations between expressions of miR-146a, IRAK1 and TRAF6 were analyzed using Pearson correlation analysis. Results: The relative expression levels of miR-146a in plasma and tissues of OLP were higher than those in healthy persons, while the relative expression levels of IRAK1 mRNA and TRAF6 mRNA in plasma and tissues of OLP were lower than those in healthy persons, and the differences were statistically significant (P<0.05). The relative expression levels of miR-146a in plasma and tissues of erosive type OLP were higher than those in non-erosive type OLP, while the relative expression levels of IRAK1 mRNA and TRAF6 mRNA were lower than those in non-erosive type OLP, and the differences were statistically significant (P<0.05). Conclusion: The expressions of miR-146a in peripheral blood and tissues of OLP were related with the degree of the lesion. It might be involved in OLP immune inflammatory response through repressing IRAK1 and TRAF6.

Key words: Oral lichen planus, MiR-146a, Peripheral blood, Tissue, Expression

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