口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (1): 24-28.DOI: 10.13701/j.cnki.kqyxyj.2017.01.006

• 基础研究论著 • 上一篇    下一篇

前导改变对大鼠颞下颌关节滑膜中iNOS及COX-2表达影响的研究

姚天华1,2,李雨轩3,4#,谭文宏3,韩会芳3,翟桐2,白乐康3,肖敏1,3*   

  1. 1. 陕西省颅颌面精准医学研究重点实验室 陕西 西安 710004;
    2. 西安交通大学口腔医院口腔综合科 陕西 西安 710004;
    3. 西安交通大学口腔医院口腔修复科 陕西 西安 710004;
    4. 银川市口腔医院口腔修复科 宁夏 银川 750001
  • 收稿日期:2016-07-29 出版日期:2017-01-25 发布日期:2017-01-22
  • 通讯作者: 肖敏,电话:029-87218541
    #为共同第一作者
  • 作者简介:姚天华(1976~ ),女,河南人,硕士,主治医师, 主要从事口腔综合类研究。
    李雨轩(1988~ ),女,陕西人,硕士,医师,主要研究方向为牙合学与颞下颌关节紊乱病。
  • 基金资助:
    陕西省社会发展科技攻关基金(编号:2012k16-10-03)

Expression of iNOS and COX-2 in Synovial Membrane of Rat with Altered Anterior Guidance.

YAO Tian-hua1,2, LI Yu-xuan3,4#, TAN Wen-hong3, HAN Hui-fang3, ZHAI Tong2, BAI Le-kang3, XIAO Min1,3*   

  1. 1. Key Laboratory of Shanxi Province for Craniofacial Precision Medicine Research, Xi’an 710004, China;2. Department of General Dentistry, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China;3. Department of Prosthodontics, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China;4. Department of Prosthodontics , Yinchuan Stomatology Hospital, Yinchuan 750001, China
  • Received:2016-07-29 Online:2017-01-25 Published:2017-01-22

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摘要： 目的:探讨实验性前导斜度改变对大鼠颞下颌关节(temporomandibular joint,TMJ)滑膜中诱导型一氧化氮合成酶(iNOS)及环氧合酶-2(COX-2)表达的影响。方法:采用低速金刚砂轮打磨大鼠上下前牙,建立前导缺失模型。在大鼠上前牙粘结舌侧增大15°、30°金属冠,建立相应前导增大15°、30°模型; 3、7、14、28 d每组各取2只大鼠一侧TMJ及另一侧TMJ滑膜组织,免疫组织化学染色(immunohistochemistry,IHC)及实时定量聚合酶链反应(Real-time polymerase chain reaction ,Real-time PCR)检测TMJ滑膜iNOS和COX-2的表达情况。结果:前导斜度增大30°组COX-2、iNOS表达较对照组显著增强(P＜0.008)。 其中COX-2在3 d时表达最高,3、7 d较28 d有统计学差异(P＜0.008)。iNOS在7 d时表达最高,较3、14、28 d表达无统计学差异。结论:iNOS及COX-2可能是前导异常造成颞下颌关节滑膜炎的重要分子机制之一。

关键词: 前导斜度, 动物模型, 颞下颌关节滑膜, 环氧合酶-2, 诱导型一氧化氮合成酶

Abstract: Objective: To investigate effects of experimentally altered anterior guidance on the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in temporomandibular joint synovium of rats. Methods: Anterior guidance deletion model was induced by eliminating the contact between anterior teeth in every direction with a diamond wheel. The anterior guidance accretion models were accomplished by cemented metal crowns which gained 15 and 30 degree on palatal side to the incisors of rats’ maxilla. Two animals in each group were sacrificed after 3, 7, 14, and 28 days. The complete TMJ of one side and TMJ synovium of the other side were obtained for analysis of iNOS and COX-2 expression by immunohistochemistry (IHC) and real-time polymerase chain reaction analysis (real-time PCR). Results: The anterior guidance in 30 degree group (group T3) showed statistical differences when compared with control group (group C) in COX-2 and iNOS mRNA expression



基金项目 陕西省社会发展科技攻关基金(编号:2012k16-10-03)
作者简介 姚天华(1976~ ),女,河南人,硕士,主治医师, 主要从事口腔综合类研究。
李雨轩(1988~ ),女,陕西人,硕士,医师,主要研究方向为牙合学与颞下颌关节紊乱病。
*通讯作者 肖敏,电话:029-87218541
#为共同第一作者
(P<0.05). In group T3, the level of COX-2 reached the highest on day 3 and there was statistical difference among specimens sacrificed after 3d, 7d and 28d (P<0.008). iNOS expression peaked on day 7, but showed no statistical differences compared with those of day 3, 14, and 28. Conclusion: COX-2 and iNOS may be involved in TMJ synovitis caused by altered anterior guidance.

Key words: Anterior, guidance, Animal, Model, Temporomandibular, joint, synovium, COX-2, iNOS

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