消退素D1治疗实验性大鼠牙周炎的研究

doi:10.13701/j.cnki.kqyxyj.2017.12.008

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1270-1273.DOI: 10.13701/j.cnki.kqyxyj.2017.12.008

消退素D1治疗实验性大鼠牙周炎的研究

唐彩金^1,2,曾启新^1*,方梅飞^1,2,马飞^1*,陶人川^1,2,Niyukty Arjal^1,2

1. 广西医科大学附属口腔医院牙周黏膜科广西南宁 530021;
2. 广西艾滋病防治研究重点实验室广西南宁 530021

收稿日期:2017-06-14 出版日期:2017-12-20 发布日期:2018-01-03
通讯作者: 曾启新,E-mail:qixinzeng@aliyun.com; 马飞,E-mail: mafei8388@163.com
作者简介:唐彩金(1989~ ),女,广西人,硕士在读,主要从事牙周黏膜病学的研究工作。
基金资助:
广西高校科学研究项目(编号:YB2014076)；广西南宁市青秀区科技局科技攻关项目(编号:2015S04)

Curative Effect of Endogenous Resolvin D1 on Periodontitis in Rats

TANG Cai-jin^1,2, ZENG Qi-xin^1*, FAGN Mei-fei^1,2, MA Fei^1*, TAO Ren-Chuan^1,2, Niyukty Arjal^1,2

1. Department of Periodontics & Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning 530021, China;
2. Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning 530021, China.

Received:2017-06-14 Online:2017-12-20 Published:2018-01-03

摘要/Abstract

摘要： 目的: 探讨消退素D1(RvD1)对治疗牙周炎的作用。方法: 取18只大鼠建立牙周炎模型,按随机化原则均分为实验甲组(100 μg/L的RvD1),实验乙组(50 μg/L的 RvD1)及阴性对照丙组(生理盐水)。所有大鼠均经尾静脉注射给药进行治疗。治疗后不同时间进行临床检查和形态学测量。采用SPSS21.0软件包对数据进行单因素方差分析。结果: 治疗后,实验甲组(100 μg/L RvD1)中牙周袋深度、牙龈指数、松动度、牙槽骨丧失量均低于阴性对照组(P<0.05)。实验乙组(50 μg/L RvD1)的以上4个指标虽均低于阴性对照组,但仅牙龈指数和松动度与阴性对照组的差异有统计学意义(P<0.05)。两实验组治疗后的牙周袋深度相近(P>0.05)。结论: 两种浓度的RvD1对实验性大鼠牙周炎有治疗作用;100 μg/L的RvD1可阻止实验性大鼠牙周炎的牙槽骨继续破坏。

关键词: 消退素D1, 牙周炎, 动物模型

Abstract: Objective: To investigate the effect of resolvin D1 (RvD1) on periodontitis in rat. Methods: The models of periodontitis were established in eighteen rats, which were randomly divided into 3 groups, experimental group A (100ng/ml RvD1), experimental group B (50ng/ml RvD1), and control group C (saline). Intervention was provided by systemic injection from the tail vein. Clinical parameters and morphological analysis were measured at different time intervals after treatment. All statistical analysis was performed using the SPSS 21.0 statistical package. Results: After 1 week of treatment, periodontal pocket depth (PD), gingival index (GI), tooth mobility (TM), and alveolar bone loss (ABL) in experimental group A (100ng/ml RvD1) were lower than those in the control group (P<0.05). For experimental group B (50ng/ml RvD1), only GI and TM showed statistical significance when compared with control group (P<0.05). PD in both experimental groups was similar. Conclusion: RvD1 might be used to prevent alveolar bone resorption in periodontitis.

Key words: Resolvin D1 , Periodontitis , Animal model

中图分类号:

R739.8

唐彩金,曾启新,方梅飞,马飞,陶人川,Niyukty Arjal. 消退素D1治疗实验性大鼠牙周炎的研究[J]. 口腔医学研究, 2017, 33(12): 1270-1273.

TANG Cai-jin, ZENG Qi-xin, FAGN Mei-fei, MA Fei, TAO Ren-Chuan, Niyukty Arjal. Curative Effect of Endogenous Resolvin D1 on Periodontitis in Rats[J]. Journal of Oral Science Research, 2017, 33(12): 1270-1273.

参考文献

[1] Kohli P, Levy BD. Resolvins and protectins: mediating solutions to inflammation [J]. British journal of pharmacology,2009,158(4)∶960-971
[2] Schwab JM, Chiang N, Arita M, et al. Resolvin E1 and protectin D1 activate inflammation-resolution programmes [J]. Nature,2007,447(7146)∶869-874
[3] Mustafa M, Zarrough A, Bolstad AI, et al. Resolvin D1 protects periodontal ligament [J]. American journal of physiology Cell physiology,2013,305(6)∶C673-679
[4] Steffens JP, Herrera BS, Coimbra LS, et al. Testosterone regulates bone response to inflammation [J]. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,2014,46(3)∶193-200
[5] 刘飞,张林,肖刚,等.生命早期负性应激对大鼠实验性牙周炎的影响[J].口腔医学研究,2017,33(3)∶244-248
[6] Cianci E, Recchiuti A, Trubiani O, et al. Human Periodontal Stem Cells Release Specialized Proresolving Mediators and Carry Immunomodulatory and Prohealing Properties Regulated by Lipoxins [J]. STEM CELLS Translational Medicine,2016,5(1)∶20-32
[7] Damgaard C, Kantarci A, Holmstrup P, et al. Porphyromonas gingivalis-induced production of reactive oxygen species, tumor necrosis factor-alpha, interleukin-6, CXCL8 and CCL2 by neutrophils from localized aggressive periodontitis and healthy donors: modulating actions of red blood cells and resolvin E1 [J]. Journal of periodontal research,2017,52(2)∶246-254
[8] Fredman G, Oh SF, Ayilavarapu S, et al. Impaired phagocytosis in localized aggressive periodontitis: rescue by Resolvin E1 [J]. PloS one,2011,6(9)∶e24422
[9] Fredman G, Serhan CN. Specialized proresolving mediator targets for RvE1 and RvD1 in peripheral blood and mechanisms of resolution [J]. The Biochemical journal,2011,437(2)∶185-197
[10] Lee CT, Teles R, Kantarci A, et al. Resolvin E1 Reverses Experimental Periodontitis and Dysbiosis [J]. Journal of immunology,2016,197(7)∶2796-806
[11] Maekawa T, Hosur K, Abe T, et al. Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3beta-C/EBPbeta pathway [J]. Nature communications,2015,6∶8272

[1]	周岳, 申玉芹. 透明质酸凝胶联合Nd:YAG激光对老年牙周炎患者牙根敏感的实验研究及临床疗效观察[J]. 口腔医学研究, 2021, 37(1): 39-42.
[2]	鲁皓, 李金凤, 苏旭, 戴江红, 陈晓涛. 负性情绪与慢性牙周炎的相关性研究[J]. 口腔医学研究, 2021, 37(1): 43-47.
[3]	刘悦, 王雷. 他汀类药物与牙周炎关系的研究进展[J]. 口腔医学研究, 2020, 36(9): 811-813.
[4]	张黎, 刘育菘, 吴芸菲, 符起亚. 基于IL-23/Th17炎症轴探讨葛根素对大鼠牙周炎牙槽骨吸收及OPG/RANKL/RANK通路的影响[J]. 口腔医学研究, 2020, 36(9): 844-849.
[5]	齐晓爽, 孟维艳. 用于牙周炎治疗的自噬调节物研究现状[J]. 口腔医学研究, 2020, 36(7): 616-618.
[6]	王昆于, 孙毓言, 张慧彦, 齐晓爽, 张震阳, 乔树伟, 向镜成, 丁佳鑫, 孟维艳. 负载D-缬氨酸水凝胶对大鼠实验性牙周炎的影响[J]. 口腔医学研究, 2020, 36(7): 658-663.
[7]	刘君瑜, 肖宇, 王翔宇, 刘艳, 李玉芳, 柳海凤, 陈宇, 包崇云. 血糖敏感型水凝胶治疗糖尿病大鼠牙周炎的实验研究[J]. 口腔医学研究, 2020, 36(6): 567-571.
[8]	吴芸菲, 张黎, 张旭凤, 符起亚. 烟草提取物辅酶Q10对牙周炎小鼠Treg/T17细胞免疫平衡的影响[J]. 口腔医学研究, 2020, 36(6): 572-576.
[9]	周伟, 汤雅, 李厚轩. 南京地区侵袭性牙周炎近6年就诊情况调查分析[J]. 口腔医学研究, 2020, 36(5): 473-476.
[10]	臧文丽, 刘俊超, 潘亚萍. 丁酸对牙周炎的影响机制研究进展[J]. 口腔医学研究, 2020, 36(4): 318-320.
[11]	田源, 陈靖, 曾凤娇, 于航, 刘霞, 刘建国, 白国辉. SD大鼠经鼻黏膜免疫牙周炎基因疫苗pVAX1-HA2-FimA-(IL-15)后Tfh细胞的变化[J]. 口腔医学研究, 2020, 36(4): 350-354.
[12]	丁小函, 岳轶云, 夏博园, 李艳, 刘歆婵, 武洲, 于维先. Mongersen对大鼠牙周炎抗炎效果观察[J]. 口腔医学研究, 2020, 36(4): 382-386.
[13]	余科, 贺晓萍, 文才, 刘旭琳, 杨健. IL-6抑制剂控制种植体愈合期炎症性骨吸收的动物实验研究[J]. 口腔医学研究, 2020, 36(3): 251-254.
[14]	杨磊, 郭留云, 程志芬, 李碧浩. Shh蛋白与慢性牙周炎炎症程度的相关性研究[J]. 口腔医学研究, 2020, 36(2): 131-134.
[15]	韩亚琨, 于程程, 于艳. 牙周炎宿主转化生长因子-β⁺调节性B细胞变化特点[J]. 口腔医学研究, 2020, 36(2): 135-138.

消退素D1治疗实验性大鼠牙周炎的研究

Curative Effect of Endogenous Resolvin D1 on Periodontitis in Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics