IL-24在口腔鳞状细胞癌中的表达及其临床意义

doi:10.13701/j.cnki.kqyxyj.2018.06.014

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 632-635.DOI: 10.13701/j.cnki.kqyxyj.2018.06.014

IL-24在口腔鳞状细胞癌中的表达及其临床意义

刘冲, 李吉辰^*, 赛音乌力吉, 朴松林, 曹博, 赵大勇

哈尔滨医科大学附属口腔医院口腔颌面外科黑龙江哈尔滨 150001

收稿日期:2017-12-20 出版日期:2018-06-20 发布日期:2018-06-21
通讯作者: 李吉辰,E-mail:lijichen@163.com
作者简介:刘冲(1989~ ),女,山东人,硕士,住院医师,主要从事口腔颌面部肿瘤的基础研究与治疗。

Expressions and Clinical Significances of IL-24 in Oral Squamous Cell Carcinoma

LIU Chong, LI Ji-chen^*, SAI Yin Wu-liji, PIAO Song-lin, CAO Bo, ZHAO Da-yong

Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, Harbin Medical University, Harbin 150001, China

Received:2017-12-20 Online:2018-06-20 Published:2018-06-21

摘要/Abstract

摘要： 目的:研究白细胞介素-24 (IL-24)在口腔鳞状细胞癌(简称鳞癌)组织中的表达并探究其临床意义。方法:分别采用免疫组化和实时聚合酶链反应(Real-time PCR)技术的方法检测75例口腔鳞癌组织和30例正常口腔黏膜组织中IL-24的蛋白和基因表达。结果:免疫组化染色显示IL-24在口腔鳞癌组织中的阳性表达率45.33%(34/75)低于正常口腔黏膜组织中的阳性表达率70.00%(21/30)(P=0.022)。IL-24的表达与口腔鳞癌的组织分化程度(P=0.002),淋巴结转移(P=0.033),临床分期(P=0.035)有密切相关性,与患者的性别(P=0.917)、年龄(P=0.812)、吸烟史(P=0.720)、饮酒史(P=0.847)及肿瘤生长部位(P=0.959)无相关性。Real-time PCR检测IL-24在不同病理分化时期口腔鳞癌组织和正常口腔黏膜组织中的基因表达水平,其高、中、低分化各组的平均表达水平分别为0.688、0.302、0.153,均低于正常口腔黏膜组织中的平均表达水平1.000(P<0.001)。结论:IL-24在口腔鳞癌组织中呈低表达,该因子的表达情况与临床信息具有一定相关性,可初步认定为口腔鳞癌的候补抑癌基因。

关键词: 口腔鳞状细胞癌, 白细胞介素-24, 免疫组织化学, 实时定量聚合酶链反应

Abstract: Objective: To study the expressions and clinical significances of interleukin-24 (IL-24) in the oral squamous cell carcinoma (OSCC). Methods: The protein and gene expressions of IL-24 were detected respectively by immunohistochemistry and real-time polymerase chain reaction (real-time PCR) technique method in 75 cases of OSCC patients and 30 cases of controls with normal oral mucosa tissues. The relationship between IL-24 expressions and the clinicopathologic features was analyzed. Results: Immunohistochemical staining showed that the positive expressions of IL-24 in the OSCC (45.33%, 34/75) were significantly lower than those of normal oral mucosa tissue (70.00%, 21/30)(P=0.022). The expressions of IL-24 were closely associated with the differentiation degree (P=0.002), lymph node metastasis (P=0.033) and clinical stage (P=0.035), but they were not correlated with the gender (P=0.917), age (P=0.812), smoking history (P=0.720), drinking history (P=0.847), and the site of lesion (P=0.959). Real-time PCR was used to detect the gene expressions of IL-24 in different pathological differentiation period of OSCC and in normal oral mucosa tissues. The average expressions of IL-24 in highly differentiated OSCC were 0.688, in moderately differentiated OSCC were 0.302, and in poorly differentiated OSCC were 0.153. All of them were lower than the average expressions of IL-24 in normal oral mucosa tissues, which were 1.000 (P<0.001). Conclusion: The expressions of IL-24 in OSCC were lower than that in normal oral mucosa tissues. This gene has certain correlation with clinical information.

Key words: Oral squamous cell carcinoma, Interleukin-24, Immunohistochemistry, Real-time PCR

刘冲, 李吉辰, 赛音乌力吉, 朴松林, 曹博, 赵大勇. IL-24在口腔鳞状细胞癌中的表达及其临床意义[J]. 口腔医学研究, 2018, 34(6): 632-635.

LIU Chong, LI Ji-chen, SAI Yin Wu-liji, PIAO Song-lin, CAO Bo, ZHAO Da-yong. Expressions and Clinical Significances of IL-24 in Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2018, 34(6): 632-635.

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IL-24在口腔鳞状细胞癌中的表达及其临床意义

Expressions and Clinical Significances of IL-24 in Oral Squamous Cell Carcinoma

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