口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (12): 1125-1128.DOI: 10.13701/j.cnki.kqyxyj.2019.12.006

• 口腔肿瘤学研究 • 上一篇    下一篇

带蒂皮瓣法构建裸鼠低氧口腔癌模型

宋勇*, 马婷婷, 王俊林, 刘彦杰, 陈裕聪, 何浒杰   

  1. 柳州市人民医院口腔科 广西 柳州 545006
  • 收稿日期:2019-06-19 出版日期:2019-12-28 发布日期:2019-12-23
  • 通讯作者: 宋勇,E-mail:songyong@whu.edu.cn
  • 作者简介:宋勇(1982~ ),男,四川人,博士,副主任医师,主要从事颌面外科临床工作。
  • 基金资助:
    国家自然科学基金(编号:81560436)

Establishment of Hypoxic Oral Cancer Model by Elevating Pedicled Flap on Dorsal Skin in Nude Mouse

SONG Yong*, MA Tingting, WANG Junlin, LIU Yanjie, CHEN Yucong, HE Hujie   

  1. Department of Stomatology, Liuzhou General Hospital. Liuzhou 545006, China.
  • Received:2019-06-19 Online:2019-12-28 Published:2019-12-23

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摘要： 目的:构建裸鼠低氧口腔癌模型,为低氧相关体内研究提供工作基础。方法:在裸鼠背部皮肤设计以单侧旋髂深动脉供血的带蒂皮瓣,切开翻瓣及原位缝合后,通过形态学观察及HIF-1α检测确定低氧最佳位点;口腔癌细胞裸鼠接种成瘤后,同法带瘤切开翻瓣,免疫组织化学染色检测肿瘤细胞HIF-1α表达变化情况。结果:裸鼠背部带蒂皮瓣长宽比在2~3:1时,皮瓣色泽正常,近颅端1/3皮肤细胞形态结构正常,HIF-1α表达明显上调,为最佳接种位点;带瘤切开翻瓣48 h后,肿瘤细胞HIF-1α表达明显上调,证实了肿瘤内环境低氧状态的动态变化。结论:带蒂皮瓣法构建裸鼠低氧口腔癌模型,方法简便易行,低氧变量施加确实可靠,为口腔癌低氧相关体内研究提供了良好的动物模型。

关键词: 口腔癌, 低氧, 裸鼠模型, 带蒂皮瓣, 低氧诱导因子

Abstract: Objective: To construct a hypoxic oral cancer model in nude mouse and provide a basis for tumor hypoxic-related studies in vivo. Methods: The pedicled skin flaps supplied by unilateral deep iliac circumflex artery were designed on the back of nude mice. After the flaps were elevated and sutured in situ, the optimal site of hypoxia was determined by morphological observation and HIF-1alpha detection. After tumor formation by inoculation of oral cancer cells subcutaneously in nude mice, flaps with tumors were incised and valved in the same way. Immunohistochemical staining was used to detect the expression of HIF-1alpha in oral cancer cells of nude mice. Results: When the length-width ratio of pedicled dorsal skin flaps in nude mice was 2-3:1, the color of the flaps was normal, the structure and morphology of skin squamous cells in distal 1/3 parts kept unchanged in which the expression of HIF-1alpha was significantly up-regulated. Furthermore, the expression of HIF-1alpha in tumor cells was significantly increased 48 hours after tumorigenesis by flapping, which confirmed the dynamic changes of hypoxic state in tumors. Conclusion: The present study provided a simple and feasible way to establish hypoxic model of oral cancer supplied by unilateral deep circumflex iliac artery in nude mice which can be applied as a good animal model for hypoxic-related research in vivo.

Key words: oral squamous cell carcinoma, hypoxia, nude mouse model, pedicled skin flap, hypoxia-inducible factor-1alpha