丹参素对牙槽骨成骨细胞细胞核因子-κB受体活化因子配体/骨保护素表达的影响研究

doi:10.13701/j.cnki.kqyxyj.2019.09.018

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (9): 894-897.DOI: 10.13701/j.cnki.kqyxyj.2019.09.018

• 口腔生物学与口腔材料学研究 • 上一篇下一篇

丹参素对牙槽骨成骨细胞细胞核因子-κB受体活化因子配体/骨保护素表达的影响研究

张晓燕^*, 刘运新, 吴铁

广东医科大学广东东莞 523808

收稿日期:2018-11-13 出版日期:2019-09-28 发布日期:2019-09-25
通讯作者: 张晓燕,E-mail:zxyivy611@163.com
作者简介:张晓燕(1978~ ),女,广东梅州人,博士,副教授,主要从事骨质疏松、炎症的研究。
基金资助:
广东省科技计划项目(编号:2013B021800071);广东省中医药局项目(编号:20121114)

Effects of Tanshinol on RANKL/OPG of Alveolar Osteoblasts

ZHANG Xiaoyan^*, LIU Yunxin, WU Tie

Guangdong Medical University, Dongguan 523808, China

Received:2018-11-13 Online:2019-09-28 Published:2019-09-25

摘要/Abstract

摘要： 目的:探讨丹参素对大鼠牙槽骨成骨细胞的细胞核因子-κB受体活化因子配体(receptor activator of NF-κB ligand, RANKL)和骨保护素(osteoprotegerin,OPG)表达的影响。方法:采用体外牙槽骨成骨细胞培养,用含不同浓度(0.10、0.50、1.00、5.00 mg/L)的丹参素血清分别作用于第4代牙槽骨成骨细胞,培养1、3和5 d后,分别通过噻唑蓝(methyl thiazol tetrazolium,MTT)、逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和Western blot法检测成骨细胞增殖率、OGP和RANKL mRNA及蛋白表达水平。结果:丹参素对牙槽骨成骨细胞增殖的影响随药物浓度和时间变化而改变,其中含5.00 mg/L丹参素的血清作用3 d后牙槽骨成骨细胞增殖率最高,OGP mRNA和蛋白表达增加(P<0.05)。结论:丹参素能通过增加OGP mRNA和蛋白表达进而促进牙槽骨成骨细胞增殖。

关键词: 成骨细胞, 细胞核因子-κB受体活化因子配体/骨保护素, 牙槽骨, 骨质疏松, 丹参素

Abstract: Objective: To investigate the effects of tanshinol on the RANKL/OPG expression of alveolar osteoblasts. Methods: Alveolar osteoblasts of SD rats were cultured by primary cell culture. The alveolar osteoblasts of the fourth generation were treated with different concentrations (0.01, 0.10, 0.50, 1.00, 5.00 mg/L) of tanshinol serum. The proliferation rate of osteoblasts and the mRNA and protein expression levels of OGP and RANKL were detected by MTT, RT-PCR, and western blotting after the use of different concentrations of tanshinol serum on 1 d, 3 d, and 5 d, respectively. Results: The effect of tanshinol on the proliferation of alveolar osteoblasts varied with concentration and time of drugs. The proliferation rate was the highest when the alveolar osteoblasts were treated with 5 mg/L tanshinol at day 3. Compared with the control group, OGP mRNA and protein expression increased (P<0.05). Conclusion: Tanshinol could promote the proliferation of alveolar osteoblasts by increasing the expression of OGP mRNA and protein.

Key words: osteoblast, OPG/RANKL, alveolar bone, osteoporosis, tanshinol

张晓燕, 刘运新, 吴铁. 丹参素对牙槽骨成骨细胞细胞核因子-κB受体活化因子配体/骨保护素表达的影响研究[J]. 口腔医学研究, 2019, 35(9): 894-897.

ZHANG Xiaoyan, LIU Yunxin, WU Tie. Effects of Tanshinol on RANKL/OPG of Alveolar Osteoblasts[J]. Journal of Oral Science Research, 2019, 35(9): 894-897.

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丹参素对牙槽骨成骨细胞细胞核因子-κB受体活化因子配体/骨保护素表达的影响研究

Effects of Tanshinol on RANKL/OPG of Alveolar Osteoblasts

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