连翘苷对牙周炎大鼠p38 MAPK/c-Fos信号通路及破骨细胞活化的影响

doi:10.13701/j.cnki.kqyxyj.2021.01.008

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (1): 33-38.DOI: 10.13701/j.cnki.kqyxyj.2021.01.008

连翘苷对牙周炎大鼠p38 MAPK/c-Fos信号通路及破骨细胞活化的影响

苏娟娟^1*, 朱永翠¹, 张文玲²

1.河南大学第一附属医院口腔科河南开封 475000;
2.河南大学淮河医院口腔科河南开封 475000

收稿日期:2020-05-11 出版日期:2021-01-28 发布日期:2021-01-22
通讯作者: *苏娟娟,E-mail:juanjuansu384@163.com
作者简介:苏娟娟(1983~ ),女,汉族,河南开封人,硕士,副主任医师,研究方向:口腔内科学。
基金资助:
河南省医学科技攻关计划联合共建项目(编号:2018020319)

Effects of Phillyrin on p38 MAPK/c-Fos Signal Pathway and Osteoclast Activation in Periodontitis Rats

SU Juanjuan^1*, ZHU Yongcui¹, ZHANG Wenling²

1. Department of Stomatology, the First Affiliated Hospital of Henan University, Kaifeng 475000, China;
2. Department of Stomatology, Huaihe Hospital of Henan University, Kaifeng 475000, China

Received:2020-05-11 Online:2021-01-28 Published:2021-01-22

摘要/Abstract

摘要： 目的: 探讨连翘苷对牙周炎大鼠p38丝裂原活化蛋白激酶(p38 MAPK)/c-Fos信号通路及破骨细胞活化的影响。方法: 建立牙周炎大鼠模型,随机分为模型组、连翘苷低剂量组、连翘苷中剂量组、连翘苷高剂量组、维生素C组,每组12只,另取12只设为对照组,分组处理后,亚甲蓝染色检测大鼠牙槽骨吸收(釉牙骨质界到牙槽嵴顶距离)情况;HE染色检测各组大鼠牙周组织病理形态;TRAP染色检测各组大鼠牙周组织中破骨细胞数目;酶联免疫吸附试验(ELISA)检测大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平;蛋白免疫印迹实验检测大鼠牙周组织p38 MAPK/c-Fos通路相关蛋白表达情况。结果: 与对照组相比,模型组大鼠牙周组织呈现上皮残缺、破裂,形态异常,牙周膜纤维排列紊乱,可见炎性细胞浸润等病理损伤,釉牙骨质界到牙槽嵴顶距离、破骨细胞数目、TNF-α、IL-6水平、牙周组织p38 MAPK/c-Fos通路相关蛋白c-Fos表达、p-p38 MAPK/p38 MAPK明显升高(P＜0.05)。与模型组相比,连翘苷低、中、高剂量组及维生素C组大鼠牙周组织病理损伤减轻,釉牙骨质界到牙槽嵴顶距离、破骨细胞数目、TNF-α、IL-6水平、牙周组织c-Fos蛋白表达、p-p38 MAPK/p38 MAPK降低,且连翘苷各组呈剂量依赖性(P＜0.05),连翘苷高剂量组与维生素C组相比,差异无统计学意义(P＞0.05)。结论: 连翘苷可下调p38 MAPK/c-Fos信号通路,抑制牙周炎症及破骨细胞活化,减轻牙周组织损伤,改善牙周炎大鼠症状。

关键词: 连翘苷, 牙周组织, 炎症, p38 MAPK/c-Fos信号, 破骨细胞活化

Abstract: Objective: To investigate the effects of phillyrin on p38 MAPK/c-Fos signal pathway and osteoclast activation in periodontitis rats. Methods: The periodontitis rat model were established and randomly divided into groups: model group, low dose phillyrin group, middle dose phillyrin group, high dose phillyrin group, and vitamin C group, with 12 in each group, and another 12 rats were set as control group. After treatment, methylene blue staining was used to detect alveolar bone resorption (distance from the border of enamel cementum to the crest of alveolar ridge). HE staining was used to detect the periodontal pathological morphology. The number of osteoclasts in periodontal tissue was detected by TRAP staining. The levels of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA). The expression of p38 MAPK/c-Fos pathway related proteins was detected by Western blot. Results: Compared with the control group, the periodontal tissue of the model group showed epithelial defect, rupture, abnormal morphology, disordered arrangement of periodontal membrane fibers, inflammatory cell infiltration, and other pathological damage, and the distance from the border of enamel cementum to the crest of alveolar ridge, the number of osteoclasts, the levels of TNF-α and IL-6, and the expressions of p38 MAPK/c-Fos pathway related proteins c-Fos and p-p38 MAPK/p38 MAPK in periodontal tissues were significantly higher (P<0.05). Compared with the model group, the pathological damage of periodontal tissue in the low, middle, and high dose phillyrin groups and vitamin C group was alleviated, the distance from the border of enamel cementum to the crest of alveolar ridge, the number of osteoclasts, the levels of TNF-α and IL-6, the expression of c-Fos protein and p-p38 MAPK/p38 MAPK in periodontal tissues were significantly lower (P<0.05), and phillyrin groups were dose-dependent (P<0.05), while there was no significant difference between high dose phillyrin group and vitamin C group (P>0.05). Conclusion: Phillyrin can down-regulate p38 MAPK/c-Fos signal pathway, inhibit periodontitis and osteoclast activation, reduce periodontal tissue damage, and improve periodontitis symptoms in rats.

Key words: phillyrin, periodontal tissue, inflammation, p38 MAPK/c-Fos signal, osteoclast activation

苏娟娟, 朱永翠, 张文玲. 连翘苷对牙周炎大鼠p38 MAPK/c-Fos信号通路及破骨细胞活化的影响[J]. 口腔医学研究, 2021, 37(1): 33-38.

SU Juanjuan, ZHU Yongcui, ZHANG Wenling. Effects of Phillyrin on p38 MAPK/c-Fos Signal Pathway and Osteoclast Activation in Periodontitis Rats[J]. Journal of Oral Science Research, 2021, 37(1): 33-38.

参考文献

[1] Li W, Zheng Q, Meng H, et al. Integration of genome-wide association study and expression quantitative trait loci data identifies AIM2 as a risk gene of periodontitis [J]. J Clin Periodontol, 2020, 47(5):583-593.
[2] Burcu K, David FL, Christopher JN, et al. Effects of smoking on non-surgical periodontal therapy in patients with periodontitis Stage Ⅲ or Ⅳ, and Grade C [J]. J Periodontol, 2020, 91(4):442-453.
[3] de Paiva Gonçalves V, Ortega AAC, Steffens JP, et al. Long-term testosterone depletion attenuates inflammatory bone resorption in the ligature-induced periodontal disease model [J]. J Periodontol, 2018, 89(4):466-475.
[4] Ishii T, Ruiz-Torruella M, Ikeda A, et al. OC-STAMP promotes osteoclast fusion for pathogenic bone resorption in periodontitis via up-regulation of permissive fusogen CD9 [J]. FASEB J, 2018, 32(7):4016-4030.
[5] Han SY, Kim YK. Berberine suppresses RANKL-induced osteoclast differentiation by inhibiting c-Fos and NFATc1 expression [J]. Am J Chin Med, 2019, 47(2):439-455.
[6] Tzeng HE, Tsai CH, Ho TY, et al. Radix paeoniae rubra stimulates osteoclast differentiation by activation of the NF-κB and mitogen-activated protein kinase pathways [J]. BMC Complement Altern Med, 2018, 18(1):132-140.
[7] 王慧,应俊,韩笑.三黄健齿汤结合西医常规疗法治疗慢性牙周炎临床研究[J].国际中医中药杂志,2018,40(5):410-413.
[8] 沈霞,徐蓉蓉,裴丽珊,等.基于网络药理学连翘清热解毒功效的分子机制研究[J].药学学报,2018,53(11):1834-1842.
[9] 祝媛,彭良哲.乙酰水杨酸预处理骨髓间充质干细胞对实验性牙周炎大鼠的治疗作用[J].基因组学与应用生物学,2020,39(1):308-313.
[10] 张震,张庆峰,李伟,等.连翘苷对创伤性骨折大鼠模型骨愈合作用的研究[J].免疫学杂志,2019,35(1):71-76.
[11] 黄捷,李庆华,任晓斌,等.辅酶Q10对大鼠实验性牙周炎的影响作用[J].中国民族民间医药,2018,27(5):25-32.
[12] 王柳然,刘歆婵,孟阳,等.Mongersen在牙周炎症微环境中抗炎作用的体外研究[J].口腔医学研究,2018,34(12):1333-1336.
[13] Chitsazi M, Faramarzie M, Sadighi M, et al. Effects of adjective use of melatonin and vitamin C in the treatment of chronic periodontitis: A randomized clinical trial [J]. J Dent Res Dent Clin Dent Prospects, 2017, 11(4):236-240.
[14] 彭曼斯,韩淋畴,何升腾,等.清胃固齿汤联合米诺环素治疗慢性牙周炎疗效以及对菌群代谢的影响[J].中华中医药学刊,2018,36(7):1739-1742.
[15] Kim K, Kim JH, Kim I, et al. Rev-erbα negatively regulates osteoclast and osteoblast differentiation through p38 MAPK signaling pathway [J]. Mol Cells, 2020, 43(1):34-47.

连翘苷对牙周炎大鼠p38 MAPK/c-Fos信号通路及破骨细胞活化的影响

Effects of Phillyrin on p38 MAPK/c-Fos Signal Pathway and Osteoclast Activation in Periodontitis Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	高睿, 闫伟军, 安晶涛, 宋冰, 邵玶. miR-146a激动剂对正畸复发过程牙周组织中炎症因子和IRAK1、TRAK6表达的影响[J]. 口腔医学研究, 2020, 36(5): 469-472.
[2]	臧文丽, 刘俊超, 潘亚萍. 丁酸对牙周炎的影响机制研究进展[J]. 口腔医学研究, 2020, 36(4): 318-320.
[3]	张贞祯, 熊婷, 郑睿, 黄佳琳, 郭玲. N-乙酰半胱氨酸对脂多糖诱导的血管内皮细胞炎症损伤的影响[J]. 口腔医学研究, 2020, 36(4): 377-381.
[4]	张丽敏, 周家敏, 张涵, 陆云洋, 魏晓渝, 张馨方, 陈白莉, 杨军英. 口腔黏膜病和口腔症状与炎症性肠病的相关性研究[J]. 口腔医学研究, 2020, 36(4): 387-392.
[5]	王素文, 赖思煜, 习利军, 王利宏. 黄芪甲苷调节人牙周膜细胞炎症反应及成骨分化的作用及机制研究[J]. 口腔医学研究, 2020, 36(2): 121-125.
[6]	岳轶云, 丁小函, 李艳, 夏博园, 王闻天, 于维先. Toll样受体4在慢性牙周炎诱发大鼠肝脏炎症中的作用[J]. 口腔医学研究, 2020, 36(2): 139-142.
[7]	胡智慧, 房维. 高迁移率族蛋白1在颞下颌关节骨关节炎中的研究进展[J]. 口腔医学研究, 2020, 36(12): 1099-1102.
[8]	周洋. 微小RNA-218-5p靶向TLR2基因对LPS所致人牙周膜干细胞炎症反应的影响[J]. 口腔医学研究, 2020, 36(12): 1108-1112.
[9]	黄佩, 乔玮玮, 孟柳燕. 自噬通过GATA4调控人牙髓细胞衰老反应[J]. 口腔医学研究, 2020, 36(12): 1142-1147.
[10]	卢永超, 王伟, 吕翱, 刘逸卿, 杜毅. 炎症微环境下骨髓间充质干细胞骨向分化中Periostin基因表达研究[J]. 口腔医学研究, 2020, 36(12): 1148-1152.
[11]	马典福, 陈帅, 肖素丽, 李萍萍, 黄晓晶. 慢性根尖周炎对大鼠血浆炎症因子及主动脉的影响[J]. 口腔医学研究, 2019, 35(9): 872-875.
[12]	吴芸菲, 张黎, 张旭凤, 符起亚. 烟草提取物对牙周炎小鼠牙周组织TLR4炎症通路的影响[J]. 口腔医学研究, 2019, 35(7): 651-656.
[13]	陈杰,徐华兴,程抒华,张旗. 细胞自噬在牙髓损伤修复过程中的作用[J]. 口腔医学研究, 2019, 35(6): 531-536.
[14]	李冬雪, 王国芳, 崔晓宇, 李小红, 轩亚茹, 王守儒. β-隐黄素对大鼠实验性牙周炎炎症因子的影响[J]. 口腔医学研究, 2019, 35(5): 471-475.
[15]	李治邦, 李琨. 雌激素影响牙周组织机制的研究进展[J]. 口腔医学研究, 2019, 35(10): 928-930.