淫羊藿素调控Ki-67及Notch-1基因表达对人口腔鳞状细胞癌细胞的促凋亡效应研究

doi:10.13701/j.cnki.kqyxyj.2021.06.006

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (6): 505-508.DOI: 10.13701/j.cnki.kqyxyj.2021.06.006

淫羊藿素调控Ki-67及Notch-1基因表达对人口腔鳞状细胞癌细胞的促凋亡效应研究

王译雪¹, 徐颖婕², 丛蓓蓓², 魏昕^1*

1.南京医科大学口腔医学院江苏南京 210029;
2.青岛大学附属青岛市口腔医院中心实验室山东青岛 266001

收稿日期:2021-03-28 出版日期:2021-06-28 发布日期:2021-06-17
通讯作者: *魏昕,E-mail:weixinart@163.com
作者简介:王译雪(1999~ ),女,山东人,本科在读,研究方向:口腔医学。
基金资助:
国家自然科学基金(编号:81902783);青岛市市南区科技发展基金(编号:2016-3-023-YY)

Apoptosis Promoting Effect of Icariin on Human Oral Squamous Cell Carcinoma Cells by Regulating Ki-67 and Notch-1 Gene Expression

WANG Yixue¹, XU Yingjie², CONG Beibei², WEI Xin^1*

1. Stomatological College of Nanjing Medical University, Nanjing 210029, China;
2. Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao 266001, China

Received:2021-03-28 Online:2021-06-28 Published:2021-06-17

摘要/Abstract

摘要： 目的:研究淫羊藿素(ICT)调控Notch-1及Ki-67基因表达对人口腔鳞状细胞癌细胞SCC-9的促凋亡效应,为ICT治疗口腔鳞状细胞癌提供实验依据。方法:分别采用4、8、16、32、64 μmol/L的ICT处理SCC-9细胞,以未采用ICT处理的细胞作为对照组。采用CCK-8法检测细胞存活率,克隆形成实验检测细胞克隆能力,利用流式细胞仪检测细胞凋亡程度,实时定量PCR检测细胞周期调控蛋白Ki-67和跨膜受体Notch-1基因表达水平。结果:经ICT处理的SCC-9细胞的增殖率、克隆数均明显低于对照组,组间比较差异均有统计学意义(P<0.01);经ICT处理的SCC-9细胞中Ki-67和Notch-1基因表达水平明显低于对照组,差异均具有统计学意义(P<0.01)。结论:ICT可通过下调Ki-67、Notch-1因子的表达水平影响SCC-9细胞的增殖及凋亡。

关键词: 淫羊藿素, Ki-67/Notch-1, 口腔鳞状细胞癌, 凋亡, 增殖

Abstract: Objective: To explore the effect of icaritin (ICT) on the apoptosis of oral squamous cell carcinoma cells-9 (SCC-9) through regulating Ki-67 and Notch-1 gene expression, and to provide experimental basis for ICT treatment of oral squamous cell carcinoma. Methods: SCC-9 cells were treated with 4, 8, 16, 32, and 64 μmol/L ICT respesctively, and equal volume of culture medium was added as blank control group. CCK-8 method was used to detect the cell survival rate. Clone formation test was used to detect the capability of cell clones. Flow cytometry was used to detect the degree of apoptosis,and real-time quantitative PCR was used to detect the mRNA expression levels of Ki-67 and Notch-1. Results: The proliferation rate and clone number of SCC-9 cells treated with ICT were significantly lower than those of control group (P<0.01). The expression levels of Ki-67 and Notch-1 in SCC-9 cells treated with ICT were significantly lower than those of control group (P<0.01). Conclusion: ICT can influence the proliferation and promote apoptosis of SCC-9 cells by down regulating the gene expression of Ki-67 and Notch-1.

Key words: ICT, Ki-67/Notch-1, squamous cell carcinoma, apoptosis, proliferation

王译雪, 徐颖婕, 丛蓓蓓, 魏昕. 淫羊藿素调控Ki-67及Notch-1基因表达对人口腔鳞状细胞癌细胞的促凋亡效应研究[J]. 口腔医学研究, 2021, 37(6): 505-508.

WANG Yixue, XU Yingjie, CONG Beibei, WEI Xin. Apoptosis Promoting Effect of Icariin on Human Oral Squamous Cell Carcinoma Cells by Regulating Ki-67 and Notch-1 Gene Expression[J]. Journal of Oral Science Research, 2021, 37(6): 505-508.

参考文献

[1] Paré A, Joly A. Oral cancer: Risk factors and management [J]. Presse Med, 2017, 46(3):320-330.
[2] Ketabat F, Pundir M, Mohabatpour F, et al. Controlled drug delivery systems for oral cancer treatment-current status and future perspectives [J]. Pharmaceutics, 2019, 11(7): 302.
[3] Liu L, Chen J, Cai X, et al. Progress in targeted therapeutic drugs for oral squamous cell carcinoma [J]. Surg Oncol, 2019, 31: 90-97.
[4] Montero PH, Patel SG. Cancer of the oral cavity [J]. Surg Oncol Clin N Am, 2015, 24(3):491-508.
[5] Ghantous Y, Abu Elnaaj I. Global incidence and risk factors of oral cancer [J]. Harefuah, 2017, 156(10): 645-649.
[6] 张瑶,张汀荣,薛蓓云.淫羊藿素对非小细胞肺癌细胞增殖、凋亡、侵袭力的影响[J].中国临床医生杂志,2018,46(6):704-707.
[7] Wo YB, Zhu DY, Hu Y, et al. Reactive oxygen species involved in prenylflavonoids,icariin and icaritin,initiating cardiac differentiation of mouse embryonic stem cells [J]. J Cell Biochem, 2008, 103: 1536-1550.
[8] Menon SS, Guruvayoorappan C, Sakthivel KM, et al. Ki-67 protein as a tumour proliferation marker [J]. Clin Chim Acta, 2019, 491:39-45.
[9] Jing Y, Zhou Q, Zhu H, et al. Ki-67 is an independent prognostic marker for the recurrence and relapse of oral squamous cell carcinoma [J]. Oncol Lett, 2019, 17(1):974-980.
[10] Deng WY, Ye SB, Luo RZ, et al. Notch-1 and Ki-67 receptor as predictors for the recurrence and prognosis of Kimura's disease [J]. Int J Clin Exp Pathol, 2014, 7(5):2402-2410.
[11] 李天客,张影,张素欣,等.基于加权基因共表达网络分析挖掘舌鳞状细胞癌的关键基因[J].口腔医学研究,2020,36(5):423-427.
[12] Fukusumi T, Califano JA. The NOTCH pathway in head and neck squamous cell carcinoma [J]. J Dent Res, 2018, 97(6):645-653.
[13] Li Y, Li YH, Chen XX. NOTCH and esophageal squamous cell carcinoma[J]. Adv Exp Med Biol, 2021, 1287:59-68.
[14] Srivastava SS, Alam H, Patil SJ, et al. Keratin 5/14-mediated cell differentiation and transformation are regulated by TAp63 and Notch-1 in oral squamous cell carcinoma-derived cells[J]. Oncol Rep, 2018, 39(5):2393-2401.
[15] Kujan O, Huang G, Ravindran A, et al. CDK4,CDK6,cyclin D1 and Notch1 immunocytochemical expression of oral brush liquid-based cytology for the diagnosis of oral leukoplakia and oral cancer [J]. J Oral Pathol Med, 2019, 48(7):566-573.
[16] Yoshida R, Nagata M, Nakayama H, et al. The pathological significance of Notch1 in oral squamous cell carcinoma[J]. Lab Invest, 2013, 93(10):1068-1081.
[17] Porcheri C, Meisel CT, Mitsiadis T, et al. Multifactorial contribution of Notch signaling in head and neck squamous cell carcinoma[J]. Int J Mol Sci, 2019, 20(6):1520.
[18] Sakamoto K, Fujii T, Kawachi H, et al. Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes insquamous neoplasms[J]. Lab Invest, 2012, 92(5):688-702.
[19] Kumar M, Nanavati R, Modi TG, et al. Oral cancer: Etiology and risk factors: A review [J]. J Cancer Res Ther, 2016, 12(2):458-463.
[20] Yang JG, Lu R, Ye XJ, et al. Icaritin reduces oral squamous cell carcinoma progression via the inhibition of STAT3 signaling [J]. Int J Mol Sci, 2017, 18(1):132.
[21] Dholam KP, Chouksey GC. Squamous cell carcinoma of the oral cavity and oropharynx in patients aged 18-45 years: A case-control study to evaluate the risk factors with emphasis on stress,diet,oral hygiene,and family history [J]. Indian J Cancer, 2016, 53(2):244-251.
[22] Jing Y, Zhou Q, Zhu H, et al. Ki-67 is an independent prognostic marker for the recurrence and relapse of oral squamous cell carcinoma [J]. Oncol Lett, 2019, 17(1):974-980.
[23] Nishi H, Shinozaki T, Tomioka T, et al. Squamous cell carcinoma of the retromolar trigone: Treatment outcomes [J]. Auris Nasus Larynx, 2018, 45(2):337-342.
[24] Lei K, Ma B, Shi P, et al. Icariin mitigates the growth and invasion ability of human oral squamous cell carcinoma via inhibiting Toll-like receptor 4 and phosphorylation of NF-κB p65 [J]. Onco Targets Ther, 2020, 13:299-307.
[25] Xu C, Huang X, Tong Y, et al. Icariin modulates the sirtuin/NF-κB pathway and exerts anti-aging effects in human lung fibroblasts [J]. Mol Med Rep, 2020, 22(5):3833-3839.
[26] Wang S, Gao J, Li Q, et al. Study on the regulatory mechanism and experimental verification of icariin for the treatment of ovarian cancer based on network pharmacology [J]. J Ethnopharmacol, 2020, 262:113189.
[27] Gharaibeh LF, Elmadany N, Alwosaibai K, et al. Notch 1 in cancer therapy: possible clinical implications and challenges [J]. Mol Pharmacol, 2020, 98(5):559-576.
[28] Li Y, Wang Z, Jin J, et al. Quercetin pretreatment enhances the radiosensitivity of colon cancer cells by targeting Notch-1 pathway [J]. Biochem Biophys Res Commun, 2020, 523(4):947-953.

淫羊藿素调控Ki-67及Notch-1基因表达对人口腔鳞状细胞癌细胞的促凋亡效应研究

Apoptosis Promoting Effect of Icariin on Human Oral Squamous Cell Carcinoma Cells by Regulating Ki-67 and Notch-1 Gene Expression

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