PMX205对致敏毒素C5a诱导RAW264.7向破骨细胞分化的影响

doi:10.13701/j.cnki.kqyxyj.2021.07.013

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (7): 637-640.DOI: 10.13701/j.cnki.kqyxyj.2021.07.013

PMX205对致敏毒素C5a诱导RAW264.7向破骨细胞分化的影响

金挺¹, 崔磊华², 王惠宁³, 侯玉帛^3*

1.温州医科大学浙江温州 325000;
2.温州医科大学附属口腔医院颌面外科浙江温州 325000;
3.温州医科大学附属口腔医院牙周科浙江温州 325000

收稿日期:2021-01-27 出版日期:2021-07-28 发布日期:2021-07-13
通讯作者: * 侯玉帛,E-mail:hou-yu-bo@wmu.edu.cn
作者简介:金挺(1998~ ),女,浙江人,本科在读,研究方向:牙龈卟啉单胞菌致病机制研究。
基金资助:
国家自然科学基金面上项目(编号:51972240);浙江省教育厅一般科研项目(编号:Y201942009);温州市科技计划项目(编号:Y20180699,Y20180700);国家大学生创新创业训练计划(编号:201810343030)

Effect of PMX205 on Osteoclastic Differentiation of RAW264.7 Cells induced by Anaphylatoxin C5a

JIN Ting¹, CUI Leihua², WANG Huining³, HOU Yubo^3*

1. Wenzhou Medical University, Wenzhou 325000, China;
2. Department of Oral and Maxillofacial Surgery, Affiliated Stomatolgical Hospital, Wenzhou Medical University, Wenzhou 325000, China;
3. Department of Periodontology, Affiliated Stomatolgical Hospital, Wenzhou Medical University, Wenzhou 325000, China

Received:2021-01-27 Online:2021-07-28 Published:2021-07-13

摘要/Abstract

摘要： 目的:探讨PMX205作用下,致敏毒素C5a对RAW264.7破骨细胞分化的影响。方法:采用MTT法检测不同浓度PMX205对RAW264.7细胞增殖水平的影响;在含核因子受体活化因子配体(RANKL)的破骨细胞诱导液条件下,实验分为3组:阴性对照组、C5a组和C5a+PMX205组,按上述条件培养5 d后,采用抗酒石酸酸性磷酸酶(TRAP)染色法检测各组破骨细胞分化水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测各组破骨细胞相关基因的表达水平。结果:MTT结果显示,PMX205对RAW264.7细胞的增殖水平无明显抑制作用。TRAP染色和qRT-PCR结果显示:10 ng/mL RANKL可成功诱导破骨细胞形成;与阴性对照组相比,1 μmol/L C5a组TRAP阳性多核细胞数目及破骨相关基因:c-fos、NFATc1和TRAF6均显著增多(P<0.01);而与C5a组相比,C5a+1 μg/mL PMX205组TRAP阳性多核细胞数目及破骨相关基因的表达明显下调(P<0.01),但仍高于阴性对照组(P<0.01)。结论:PMX205可通过C5a-C5aR轴抑制C5a诱导的RAW264.7破骨细胞分化。

关键词: 牙周炎, C5a, RAW264.7, PMX205, 破骨细胞

Abstract: Objective: To investigate the effect of PMX205 on osteoclastic differentiation of RAW264.7 cells induced by anaphylatoxin C5a. Methods: The viability of RAW264.7 treated by PMX205 was detected with MTT assays, and then RAW264.7 were divided into three groups: control group; C5a group, in which cells were induced osteoclastogenic differentiation in medium supplemented with 10 ng/mL RANKL and 1 μmol/L C5a; C5a+PMX205 group, in which cells were treated with 1 μg/mL PMX205, 10 ng/mL RANKL, and 1 μmol/L C5a. The differentiation of RAW264.7 to osteoclast-like cells was quantified by tartrate-resistant acid phosphatase (TRAP) staining, and genes expression associated with osteoclastic differentiation were assessed by real-time quantitative polymerase chain reaction (qRT-PCR). Results: PMX205 significantly inhibited the osteoclastic differentiation induced by C5a in RAW264.7 cells without cytotoxicity. The inhibitory effects of PMX205 on differentiation-related signaling molecules resulted in significant repression of c-fos, TRAF6, and NFATc1 expression, compared with the C5a group (P<0.01), which was still higher than that of control group (P<0.01). Conclusion: PMX205 decreased osteoclastic differentiation of RAW264.7 induced by C5a through C5a-C5aR axis.

Key words: periodontitis, C5a, RAW264.7, PMX205, Osteoclast

金挺, 崔磊华, 王惠宁, 侯玉帛. PMX205对致敏毒素C5a诱导RAW264.7向破骨细胞分化的影响[J]. 口腔医学研究, 2021, 37(7): 637-640.

JIN Ting, CUI Leihua, WANG Huining, HOU Yubo. Effect of PMX205 on Osteoclastic Differentiation of RAW264.7 Cells induced by Anaphylatoxin C5a[J]. Journal of Oral Science Research, 2021, 37(7): 637-640.

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PMX205对致敏毒素C5a诱导RAW264.7向破骨细胞分化的影响

Effect of PMX205 on Osteoclastic Differentiation of RAW264.7 Cells induced by Anaphylatoxin C5a

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