3种根管封闭剂体外细胞相容性、促成骨性及抗菌性的实验研究

doi:10.13701/j.cnki.kqyxyj.2022.11.014

摘要/Abstract

摘要： 目的: 评价3种根管封闭剂iMTA、BioRoot RCS和AH plus的体外细胞相容性、促成骨性及抗菌性。方法: 设对照组和3种根管封闭剂实验组,并制备浸提液,将浸提液进行梯度稀释为100%、75%、50%、25%,用不同浓度的浸提液培养L929细胞,CCK-8法测定24、48、72 h吸光度(A)值并计算各组细胞相对增殖率;同时用浸提液培养MC3T3-E1细胞,比较3种封闭剂的7 d的碱性磷酸酶活性和28 d时矿化结节形成能力;采用直接接触法,将新鲜封闭剂与粪肠球菌菌液混合,比较各组封闭剂的抗菌性。结果: 随浸提液浓度增加,细胞相对增殖率降低;相同的接触时间和浓度下iMTA和BioRoot RCS组的细胞相对增殖率高于AH plus组(P＜0.01);iMTA和BioRoot RCS组碱性磷酸酶活性和矿化结节形成能力显著高于AH plus组(P＜0.01),且iMTA和BioRoot RCS组比较差异无统计学意义(P＜0.01);相比于对照组,iMTA可显著减少粪肠球菌数量(P＜0.01)。结论: iMTA细胞毒性与浸提液浓度呈负相关且与接触时间呈正相关,iMTA和BioRoot RCS的促成骨性能相似,对粪肠球菌有一定的抗菌作用。

关键词: 根管封闭剂, 细胞相容性, 成骨, 抗菌

Abstract: Objective: To evaluate the in vitro cytocompatibility and osteogenic and antibacterial activity of iMTA, BioRoot RCS, and AH Plus. Methods: The control group and experimental group of three root canal sealers were set. The extraction was gradient diluted by 100%, 75%, 50%, and 25%, respectively. L929 cells were cultured with different concentrations of extracts. The A values at 24, 48, and 72h were determined by CCK-8, and the relative proliferation rate of each group was calculated. MC3T3-E1 cells were cultured with extracts. The alkaline phosphatase (ALP) activity and mineralization-promoting nodule formation potential were compared after 7 days and 28 days. Using direct contact method, fresh sealer was mixed with Enterococcus faecalis solution to compare the antibacterial activity of each group. Results: With the increase of extraction concentration, the relative proliferation rate of cells were decreased. The relative proliferation rates of iMTA and BioRoot RCS group were higher than that of AH plus group under the same contact time and concentration (P<0.01). The ALP activity and mineralization-promoting nodule formation ability of iMTA and BioRoot RCS were significantly higher than those of AH plus (P<0.01), and there was no significant difference between iMTA and BioRoot RCS groups (P<0.01). Compared with the control group, iMTA significantly reduced the number of Enterococcus faecalis (P<0.01). Conclusion: The cytotoxicity of iMTA is negatively correlated with the concentration of the extract and positively correlated with the contact time. iMTA and BioRoot RCS have similar osteogenic energy, and have certain antibacterial effect on Enterococcus faecalis.

Key words: root canal sealer, cytocompatibility, osteogenesis, antibacterial

杨习良, 郑天霞, 杨诺亚, 尹紫涵, 白宇宏. 3种根管封闭剂体外细胞相容性、促成骨性及抗菌性的实验研究[J]. 口腔医学研究, 2022, 38(11): 1071-1075.

YANG Xiliang, ZHENG Tianxia, YANG Nuoya, YIN Zihan, BAI Yuhong. Study on Cytocompatibility, Osteogenic Potential, and Antibacterial Activity of Three Root Canal Sealers in Vitro[J]. Journal of Oral Science Research, 2022, 38(11): 1071-1075.

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