柠檬精油对变异链球菌产酸及乳酸脱氢酶活性影响的实验研究

doi:10.13701/j.cnki.kqyxyj.2018.01.007

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (1): 27-31.DOI: 10.13701/j.cnki.kqyxyj.2018.01.007

柠檬精油对变异链球菌产酸及乳酸脱氢酶活性影响的实验研究

孙艳伟¹, 刘雅丽², 赵晓雪¹, 侯菲菲¹, 张向宇^1*

1. 天津医科大学口腔医学院天津 300000;
2. 天津市塘沽口腔医院牙体牙髓科天津 300450

收稿日期:2017-09-15 出版日期:2018-01-28 发布日期:2018-01-26
通讯作者: 张向宇,电话:022-23332102
作者简介:孙艳伟 (1989~ ),男,河南人,硕士,主要从事天然防龋剂的开发及儿童口腔医学方面的研究。
基金资助:
天津市应用基础及前沿技术研究计划(编号:10JCYBJCl3600)

Effects of Lemon Essential Oil on Acid Production and Activity of Lactic Dehydrogenase in Streptococcus Mutans

SUN Yan-wei¹, LIU Ya-li², ZHAO Xiao-xue¹, HOU Fei-fei¹, ZHANG Xiang-yu^1*

1. School of Stomatology, Tianjing Medical University. Tianjing 300000, China;
2. Department of Endodontics, Tanggu Stomatology Hospital. Tianjing 300450, China

Received:2017-09-15 Online:2018-01-28 Published:2018-01-26

摘要/Abstract

摘要： 目的:探讨亚抑菌浓度下柠檬精油(lemon essential oil ,LEO)对变异链球菌产酸及乳酸脱氢酶(lactate dehydrogenase,LDH)活性的影响,为LEO在口腔中的应用提供理论依据。方法:根据LEO的最小抑菌浓度(minimal inhibitory concentration,MIC),采用二倍稀释法用培养基配制1/8MIC、1/4MIC、1/2MIC的LEO的稀释液,对照组只含有培养基。加入变异链球菌培养24 h后,测定培养液的pH变化值(△pH),还原性辅酶Ⅰ氧化法测定LDH的活性,RT-PCR检测产酸相关基因ldh的表达量。结果:在亚抑菌浓度下,随着LEO浓度的升高,实验组△pH从1.423降到0.067, LDH活性从33.279 U/mg降到 13.367 U/mg,相比对照组差异均有统计学意义(P＜0.01); RT-PCR显示实验组ldh基因的表达量相比对照组显著下调,差异有统计学意义(P＜0.05)。结论:亚抑菌浓度下LEO能够在不影响细菌生长的情况下,通过抑制变异链球菌ldh基因的表达从而降低LDH的活性,减少酸的产生。

关键词: 柠檬精油, 变异链球菌, 产酸, 乳酸脱氢酶

Abstract: Objective: To investigate the effects of LEO on the acid production and activity of lactate dehydrogenase (LDH) in Streptococcus mutans, and to provide a theoretical basis for the application of lemon essential oil (LEO) in oral cavity. Methods: According to the minimal inhibitory concentration (MIC) of LEO, the dilution of 1/8MIC, 1/4MIC, 1/2MIC of LEO and the control group (only contained medium) were prepared by using two-fold dilution method. Streptococcus mutans was added into each group and cultivated for 24h. △pH of medium in each group was determined. The method of oxidation of reduction coellzymeⅠand RT-PCR were respectively adopted to evaluate the activity of LDH and the expression of ldh gene related to acid production. Results: With increasing concentrations of LEO, a dose-dependent decline of △pH (1.423-0.067) and the activity of LDH (33.279-13.367 U/mg) was observed in the LEO group compared to the control group at sub-MIC levels (P<0.01). Compared to the control group, the expression of ldh gene significantly decreased (P<0.05). Conclusion: Sub-MICs of LEO inhibited the activity of LDH and reduced acid production by down-regulating the expression of ldh gene without affecting bacterial growth.

Key words: Lemon essential oil, Streptcoccus mutans, Acid production, Lactate dehydrogenase

中图分类号:

R780.1

孙艳伟, 刘雅丽, 赵晓雪, 侯菲菲, 张向宇. 柠檬精油对变异链球菌产酸及乳酸脱氢酶活性影响的实验研究[J]. 口腔医学研究, 2018, 34(1): 27-31.

SUN Yan-wei, LIU Ya-li, ZHAO Xiao-xue, HOU Fei-fei, ZHANG Xiang-yu. Effects of Lemon Essential Oil on Acid Production and Activity of Lactic Dehydrogenase in Streptococcus Mutans[J]. Journal of Oral Science Research, 2018, 34(1): 27-31.

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柠檬精油对变异链球菌产酸及乳酸脱氢酶活性影响的实验研究

Effects of Lemon Essential Oil on Acid Production and Activity of Lactic Dehydrogenase in Streptococcus Mutans

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