口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (1): 22-26.DOI: 10.13701/j.cnki.kqyxyj.2018.01.006

• 口腔干细胞研究 • 上一篇    下一篇

山羊颌骨骨髓间充质干细胞与纳米晶胶原骨材料体外复合培养的实验研究

李冬梅1*, 刘新晖2, 李庆星1   

  1. 1. 河北医科大学第一医院口腔科 河北 石家庄 050031;
    2. 南京市江宁医院骨科 江苏 南京 211100
  • 收稿日期:2017-08-29 出版日期:2018-01-28 发布日期:2018-01-26
  • 通讯作者: 李冬梅,E-mail:lidongmei19701@163.com
  • 作者简介:李冬梅(1970~ ),女,河北省阳原县人,学士,副主任医师,主要从事口腔疾病诊治工作。
  • 基金资助:
    河北省卫生厅科研基金项目(编号08069)

Repair of Rabbits Critical Mandibular Defects with BMSCs/NHAC Complex

LI Dong-mei1*, LIU Xin-hui2, LI Qing-xing1.   

  1. 1. Department of Stomatology, the First Hospital of Hebei Medical University,Shijiazhuang 050031, China;
    2. Department of Orthopedics, Jiangning Hospital in Nanjing City, Nanjing 211100, China
  • Received:2017-08-29 Online:2018-01-28 Published:2018-01-26

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摘要： 目的:探讨山羊骨髓间充质干细胞(BMSCs)与纳米晶胶原骨材料(NHAC)体外复合培养的结合情况,并初步探讨BMSCs/NHAC复合物对体内兔下颌骨缺损修复的效果。方法:第3代山羊BMSCs与NHAC共培养,应用MTS检测进细胞增殖、碱性磷酸酶活性检测ALP活性、实时荧光定量PCR(qRT-PCR)检测成骨基因。建立兔下颌骨缺损模型,于缺损处植入成骨诱导分化的BMSCs/NHAC复合物,应用Goldner三色染色实验和micro-CT评估骨再生情况。结果:在培养第5天和第7天,共培养组A490值均显著高于正常组(P＜0.05)。共培养诱导组ALP活性显著高于正常诱导组(P＜0.05)。共培养诱导组中OCN、Runx2、Osterix mRNA表达水平均显著高于正常诱导组(P＜0.05)。与Block组相比,NHAC组和BMSCs/NHAC组骨体积密度均显著增加(P＜0.05)。与NHAC组相比,BMSCs/NHAC组骨体积密度显著增加(P＜0.05)。结论:NHAC能够为BMSCs的粘附、增殖和分化提供合适的环境,且BMSCs/NHAC复合物能够促进兔下颌缺损部位的骨再生。

关键词: 骨髓间充质干细胞, 纳米晶胶原骨材料, 组织工程

Abstract: Objective: To investigate the effect of BMSCs/NHAC complex on repair of rabbit's critical mandibular defects. Methods: MTS experiments were used to detect cell proliferation. ALP activity was measured by alkaline phosphatase activity assay. The osteogenic gene was detected by qRT-PCR. The complex was implanted into the mandibular critical defect of rabbit. Goldner trichrome staining test and micro-CT were used to evaluate bone regeneration. Results: OD490 values were significantly higher in the co-culture group than that in the normal group (P<0.05). ALP activity in the co-culture-induced group was significantly higher than that in the normal induction group, as well as the expression levels of OCN, Runx2, and Osterix mRNA (P<0.05). Compared with the black group, the bone volume and bone density of NHAC group and BMSCs/NHAC group were significantly increased (P<0.05). Conclusion: BMSCs/NHAC complexes can promote the bone regeneration in the mandibular critical defect of rabbit.

Key words: Bone marrow mesenchymal stem cells, Nanocrystalline collagen bone material, Tissue engineering

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