牙龈卟啉单胞菌对大鼠胎盘及仔鼠出生体重的影响

doi:10.13701/j.cnki.kqyxyj.2018.05.009

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (5): 495-499.DOI: 10.13701/j.cnki.kqyxyj.2018.05.009

牙龈卟啉单胞菌对大鼠胎盘及仔鼠出生体重的影响

梁珊珊, 邢文燕, 季耀庭, 江汉, 杜民权^*

武汉大学口腔医学院口腔生物医学工程教育部重点实验室湖北武汉 430079

收稿日期:2018-01-12 出版日期:2018-05-28 发布日期:2018-05-29
通讯作者: 杜民权,E-mail:duminquan@whu.edu.cn
作者简介:梁珊珊(1991~ ),女,湖北襄阳人,硕士在读,主要从事口腔预防医学研究。
基金资助:
国家自然科学基金(编号:81371145)

Effects of Porphyromonas Gingivalis Infection on Placental Tissue and Birth Weight of Neonates in Rats.

LIANG Shan-shan, XING Wen-yan, JI Yao-ting, JIANG Han, DU Min-quan^*

Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2018-01-12 Online:2018-05-28 Published:2018-05-29

摘要/Abstract

摘要： 目的:研究牙龈卟啉单胞菌(P.gingivalis)感染对大鼠胎盘组织Fas、Fas配体(Fas ligand,FasL)、Toll样受体(Toll-like receptor,TLR)2、TLR4表达水平及对新生仔鼠出生体重的影响。方法:将大鼠随机分为P.gingivalis感染组、阴性对照组和空白对照组,每组13只。采用尾静脉注射法建立P.gingivalis感染的孕鼠模型。记录各组新生仔鼠的出生体重和窝重并收集各组孕鼠在孕18 d的胎盘。采用免疫组织化学法和Western blot法检测各组胎盘Fas、FasL、TLR2和TLR4表达情况。结果:P.gingivalis感染组与阴性对照组相比,其新生仔鼠出生体重显著减轻;孕鼠胎盘组织中TLR4、Fas、FasL的表达显著增强,TLR2无明显变化。结论:在P.gingivalis感染的孕鼠模型中,P.gingivalis感染可引起新生仔鼠较低出生体重,还可引起胎盘组织TLR4、Fas、FasL表达增强。提示罹患牙周病的孕妇娩出低体重儿,可能与P.gingivalis引起的胎盘组织TLR4、Fas、FasL的异常调节有关。

关键词: 牙龈卟啉单胞菌, 早产低体重, 胎盘

Abstract: Objective: To investigate the effects of Porphyromonas gingivalis infection on expressions of placental Fas ligand (Fas L), Toll-like receptor (TLR)2 and TLR4, and birth weight of neonates in rats. Methods: Female rats were randomly divided into 3 groups: P.gingivalis-infected group, negative control group, and blank control group (n=13 for each group). Murine model of P.gingivalis infection was established by the tail vein injection. Birth weight of every neonate and litter weights was recorded, and the placentas were obtained at gestational day of 18. The expression levels of placental Fas, FasL, TLR2, and TLR4 were detected by Western blot analysis and immunohistochemical staining. Results: The newborn rats showed significantly lower birth weight in P.gingivalis-infected group. Furthermore, Fas, FasL, and TLR4 in placental tissues were significantly increased following P.gingivalis infection. However, the expression of TLR2 had no significant difference between P.gingivalis-infected group and negative control group. Conclusion: In our murine model, with elevated expression of Fas, FasL and TLR4 in placenal tissues, P.gingivalis infection induces lower birth weight of neonates. The results indicate that pregnant woman suffering from periodontal disease, with delivery of low birth weight babies, might be related to P.gingivalis-induced abnormally regulation of placental Fas, FasL and TLR4.

Key words: Porphyromonas gingivalis, Preterm low birth weight, Placenta

梁珊珊, 邢文燕, 季耀庭, 江汉, 杜民权. 牙龈卟啉单胞菌对大鼠胎盘及仔鼠出生体重的影响[J]. 口腔医学研究, 2018, 34(5): 495-499.

LIANG Shan-shan, XING Wen-yan, JI Yao-ting, JIANG Han, DU Min-quan. Effects of Porphyromonas Gingivalis Infection on Placental Tissue and Birth Weight of Neonates in Rats.[J]. Journal of Oral Science Research, 2018, 34(5): 495-499.

参考文献

[1] Hughes MM, Black RE, Katz J. 2500-g low birth weight cutoff: history and implications for future research and policy [J]. Matern Child Health J, 2017, 21(2)∶283-289
[2] Corbella S, Taschieri S, Francetti L, et al. Periodontal disease as a risk factor for adverse pregnancy outcomes: a systematic review and meta-analysis of case-control studies [J]. Odontology, 2012, 100(2)∶232-240
[3] Teshome A, Yitayeh A. Relationship between periodontal disease and preterm low birth weight: systematic review [J]. Pan Afr Med J, 2016, 24∶215
[4] Vanterpool SF, Been JV, Houben ML, et al. Porphyromonas gingivalis within placental villous mesenchyme and umbilical cord stroma is associated with adverse pregnancy outcome [J]. PLoS One, 2016, 11(1)∶e0146157
[5] Ao M, Miyauchi M, Furusho H, et al. Dental infection of porphyromonas gingivalis induces preterm birth in mice [J]. PLoS One, 2015, 10(8)∶e0137249
[6] Guttmacher AE, Maddox YT, Spong CY. The human placenta project: placental structure, development, and function in real time [J]. Placenta, 2014, 35(5)∶303-304
[7] Li L, Ren H, Li Y, et al. Effect of porphyromonas gingivalis infection on human trophoblast cells proliferation and apoptosis [J]. Journal of Oral Science Research, 2016, 32(2)∶143-146
[8] Macpherson AJ, de Agüero MG, Ganal-Vonarburg SC. How nutrition and the maternal microbiota shape the neonatal immune system [J]. Nat Rev Immunol, 2017, 17(8)∶508-517
[9] Ide M, Papapanou PN. Epidemiology of association between maternal periodontal disease and adverse pregnancy outcomes--systematic review [J]. J Clin Periodontol, 2013, 40 Suppl 14∶S181-S194
[10] Turton M, Africa CW. Further evidence for periodontal disease as a risk indicator for adverse pregnancy outcomes [J]. Int Dent J, 2017, 67(3)∶148-156
[11] Hirsch E, Wang H. The molecular pathophysiology of bacterially induced preterm labor: insights from the murine model [J]. J Soc Gynecol Investig, 2005, 12(3)∶145-155
[12] Vinturache AE, Gyamfi-Bannerman C, Hwang J, et al. Maternal microbiome - A pathway to preterm birth [J]. Semin Fetal Neonatal Med, 2016, 21(2)∶94-99
[13] Arce RM, Barros SP, Wacker B, et al. Increased TLR4 expression in murine placentas after oral infection with periodontal pathogens [J]. Placenta, 2009, 30(2)∶156-162
[14] Inaba H, Kuboniwa M, Sugita H, et al. Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts [J]. Infect Immun, 2012, 80(8)∶2847-2857
[15] Ren H, Li Y, Jiang H, et al. Interferon-gamma and Fas are involved in Porphyromonas gingivalis-induced apoptosis of human extravillous trophoblast-derived HTR8/SVneo cells via extracellular signal-regulated kinase 1/2 pathway [J]. J Periodontol, 2016, 87(11)∶e192-e199
[16] Qiu Q, Yang M, Tsang BK, et al. Fas ligand expression by maternal decidual cells is negatively correlated with the abundance of leukocytes present at the maternal-fetal interface [J]. J Reprod Immunol, 2005, 65(2)∶121-132

牙龈卟啉单胞菌对大鼠胎盘及仔鼠出生体重的影响

Effects of Porphyromonas Gingivalis Infection on Placental Tissue and Birth Weight of Neonates in Rats.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张慧彦, 李保胜, 张震阳, 韩春雨, 李尊泰, 齐晓爽, 付齐月, 孟维艳. D/L-缬氨酸对牙龈卟啉单胞菌及其生物膜的作用[J]. 口腔医学研究, 2020, 36(7): 648-653.
[2]	邬扬绚, 向黎, 黄志强, 褚金海. 釉基质蛋白衍生物调节牙龈卟啉单胞菌感染牙周膜干细胞成骨分化的作用及机制[J]. 口腔医学研究, 2020, 36(6): 534-538.
[3]	王腾飞, 徐燕, 徐涵颖, 韩旭, 沈继龙, 程婷. 卵黄抗体缓释型纳米羟基磷灰石的制备及其抗菌性能的研究[J]. 口腔医学研究, 2020, 36(6): 591-594.
[4]	张源, 李新月. 脂多糖抑制牙周膜细胞骨唾液酸蛋白表达中微小RNA的筛选与验证[J]. 口腔医学研究, 2020, 36(5): 477-480.
[5]	田源, 陈靖, 曾凤娇, 于航, 刘霞, 刘建国, 白国辉. SD大鼠经鼻黏膜免疫牙周炎基因疫苗pVAX1-HA2-FimA-(IL-15)后Tfh细胞的变化[J]. 口腔医学研究, 2020, 36(4): 350-354.
[6]	许志强, 曾秀峰, 洪少楠, 曾秀霞, 贺于奇, 黄俊徽. 不同管径二氧化钛纳米管的抗菌性能研究[J]. 口腔医学研究, 2020, 36(3): 230-234.
[7]	王素文, 赖思煜, 习利军, 王利宏. 黄芪甲苷调节人牙周膜细胞炎症反应及成骨分化的作用及机制研究[J]. 口腔医学研究, 2020, 36(2): 121-125.
[8]	孙天祥, 苗棣, 王宝彦, 陈悦. 高糖对牙龈卟啉单胞菌LPS引发牙周膜细胞炎症反应的影响[J]. 口腔医学研究, 2020, 36(12): 1123-1127.
[9]	左源, 李维善. 牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞表达小凹蛋白-1的影响[J]. 口腔医学研究, 2020, 36(1): 75-78.
[10]	聂然, 周延民. 慢性牙周炎与阿尔茨海默病相关性的研究进展[J]. 口腔医学研究, 2019, 35(5): 419-422.
[11]	李雨阳,孟维艳. 牙龈卟啉单胞菌抵抗固有免疫防御的研究进展[J]. 口腔医学研究, 2019, 35(2): 113-115.
[12]	叶兴如, 徐燕, 庞罡, 王莹, 周莉莉, 蒋鹏, 沈继龙, 王荣海. RT-qPCR检测CPC对中重度慢性牙周炎患者口内环境中红色复合体的影响[J]. 口腔医学研究, 2018, 34(8): 852-856.
[13]	郭海盈, 任洪芋, 季耀庭, 江汉, 杜民权. p-Akt对牙龈卟啉单胞菌诱导的人胚胎滋养层细胞凋亡的影响[J]. 口腔医学研究, 2018, 34(5): 500-504.
[14]	唐路, 薛栋, 白雨豪, 王鹏程, 周维, 李文婷, 董岩, 曾红燕, 赵颖. 牙龈卟啉单胞菌对血管平滑肌细胞钙化作用的研究[J]. 口腔医学研究, 2018, 34(2): 130-133.
[15]	黄琳砚,赖仁发,黄怡. 牙周炎与动脉粥样硬化信号相互作用的关联影响[J]. 口腔医学研究, 2018, 34(12): 1271-1273.