南疆维吾尔族儿童龋病及16S rRNA高通量测序菌斑微生物群落结构

doi:10.13701/j.cnki.kqyxyj.2018.12.011

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (12): 1307-1311.DOI: 10.13701/j.cnki.kqyxyj.2018.12.011

南疆维吾尔族儿童龋病及16S rRNA高通量测序菌斑微生物群落结构

仵楠,代海涛^*,徐江

石河子大学医学院第一附属医院口腔科新疆石河子 832008

收稿日期:2018-06-13 出版日期:2018-12-28 发布日期:2018-12-27
通讯作者: 代海涛,E-mail:48440045@qq.com
作者简介:仵楠(1982~ ),女,新疆人,硕士,副主任医师,主要从事牙体牙髓、牙周疾病研究。
基金资助:
2017年石河子大学医学院第一附属医院院级课题(编号:YB201703)

Study on Caries and Microbial Community Structure by 16S rRNA High-throughput Sequencing in Uighur Children of Southern Xinjiang.

WU Nan, DAI Hai-tao^*, XU Jiang

The First Affiliated Hospital, School of Medicine, Shihezi University. Shihezi 832008, China.

Received:2018-06-13 Online:2018-12-28 Published:2018-12-27

摘要/Abstract

摘要： 目的: 分析南疆维吾尔民族儿童患龋情况及微生物学角度研究龋病可能高发的原因。方法: 通过分层整群抽样随机抽取南疆第三师51团5个连队幼儿园3~5岁维吾尔族儿童296名调查患龋情况及充填率,应用Illumina Miseq高通量测序技术分析高龋儿童、无龋儿童口腔微生物群落结构及多样性。结果: 南疆第三师51团维吾尔族3~5岁儿童总患龋率 83.4% 、龋均(3.07±3.22) 、龋补充填率2.07%。5岁组儿童患龋率、龋均明显高于3岁组,不同性别儿童患龋率和龋均之间差异均无统计学意义。无龋组与高龋组口腔菌斑呈微生物多样性,共98个outs,归属于12个门、18个纲、25个目、34个科、60个属。无龋组微生物多样性较高龋组丰富,Streptococcaceae,Porphyromonadaceae、Fusobacteria、Veillonellaceae、peptostreptococcaceae在两组所有样本中均存在,在高龋组中构成比高于无龋组。结论: 南疆维吾尔族儿童患龋率明显高于国内平均水平,应加强对南疆地区群众的口腔资源投入,当地维吾尔族儿童中高龋和无龋人群口腔微生物群落结构存在一定的差异,高龋组中优势菌(Streptococcaceae,Porphyromonadaceae、Fusobacteria、Veillonellaceae、peptostreptococcaceae)对龋病发生发展的作用还有待进一步的研究。

关键词: 龋病, 菌斑, 微生物群落结构, 16S, rRNA, 高通量测序

Abstract: Objective: To study the condition of dental caries in Uighur children in southern Xinjiang and analyze the reasons of high incidence of caries in microbiology.Methods: Caries and filling rate of 296 Uygur children aged 3-5 in 5 company kindergartens in southen xinjiang were randomly selected.The microbiological community structure and diversity of oral cavity in children were analyzed by illumina misequencing platform.Results: Tooth decay rate was 83.4%, average caries was 3.07±3.22, and supplementary filling rate was 2.07%.The tooth decay rate and average caries of 5 year old group were higher than those of 3 year old group.There was no significant difference between the rate of caries and the average of caries in children with different gender.The dental plaque of two groups was various.There were 98 outs, belongs to12 phylum, 18 classes, 25 orders, 34 families, and 60 genus.Streptococcaceae, porphyromonadaceae, fusobacteria, veillonellaceae, and peptostreptococcaceae were richer in high caries.Conclusion: The incidence of dental caries in Uygur children in southern Xinjiang is higher than the domestic average, and it is necessary to strengthen the input of oral resources in southern Xinjiang.There are some differences in the structure of oral microorganism community between caries and non-caries.The effects of streptococcaceae, porphyromonadaceae, fusobacteria, veillonellaceae, and peptostreptococcaceae on caries development need to be further studied.

Key words: Caries, Dental plaque, Microbial community structure, 16S rRNA, High-throughput sequencing

仵楠,代海涛,徐江. 南疆维吾尔族儿童龋病及16S rRNA高通量测序菌斑微生物群落结构[J]. 口腔医学研究, 2018, 34(12): 1307-1311.

WU Nan, DAI Hai-tao, XU Jiang. Study on Caries and Microbial Community Structure by 16S rRNA High-throughput Sequencing in Uighur Children of Southern Xinjiang.[J]. Journal of Oral Science Research, 2018, 34(12): 1307-1311.

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南疆维吾尔族儿童龋病及16S rRNA高通量测序菌斑微生物群落结构

Study on Caries and Microbial Community Structure by 16S rRNA High-throughput Sequencing in Uighur Children of Southern Xinjiang.

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