口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (6): 591-594.DOI: 10.13701/j.cnki.kqyxyj.2020.06.020

• 口腔材料学研究 • 上一篇    下一篇

卵黄抗体缓释型纳米羟基磷灰石的制备及其抗菌性能的研究

王腾飞1, 徐燕1*, 徐涵颖1, 韩旭1, 沈继龙3, 程婷2   

  1. 1.安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室 安徽 合肥 230032;
    2.安徽安科生物工程(集团)股份有限公司 安徽 合肥 230032;
    3.安徽医科大学人兽共患病安徽省重点实验室 安徽 合肥 230032
  • 收稿日期:2019-09-27 出版日期:2020-07-03 发布日期:2020-07-06
  • 通讯作者: *徐燕,E-mail:173236344@qq.com
  • 作者简介:王腾飞(1992~ ),男,安徽阜阳人,硕士在读,主要从事牙周临床工作。
  • 基金资助:
    安徽省教育厅自然科学重大项目(编号:KJ2017ZD17)

Preparation of Yolk Antibody Sustained-release Nano-hydroxyapatite and Its Antibacterial Properties

WANG Tengfei1, XU Yan1*, XU Hanying1, HAN Xu1, SHEN Jilong3, CHENG Ting2   

  1. 1. Department of Periodontology, Stomatologic Hospital & College, Anhui Medical University, Key Lab of Oral Diseases Research of Anhui Province, Hefei 230032, China;
    2. Anhui Anke Biotechnology (Group) Co. Ltd, Hefei 230032, China;
    3. Key Lab of Zoonoses of Anhui Province, Anhui Medical University, Hefei 230032, China
  • Received:2019-09-27 Online:2020-07-03 Published:2020-07-06

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摘要： 目的: 制备具有卵黄抗体缓释功能的纳米羟基磷灰石复合材料,并进行体外抗菌性能检测。方法: 采用扫描电子显微镜(SEM)对纳米羟基磷灰石(nano-hydroxyapatite,NHA)的结构进行观察。对NHA进行抗牙龈卟啉单胞菌卵黄抗体(抗Pg-IgY)装载,并研究IgY-NHA复合材料的药物缓释行为。选取牙龈卟啉单胞菌(Porphyromonas gingival,Pg)作为实验菌株,研究IgY-NHA复合材料的最小抑菌浓度(minimal inhibitory concentration,MIC)和IgY-NHA复合材料对Pg的生长抑制作用来评价IgY缓释型纳米羟基磷灰石的抗菌性能。结果: NHA为颗粒状纳米结构组成的多孔结构;随着IgY装载浓度的增加,NHA的吸附量也随之显著增加,当IgY装载浓度为4 g/L时,吸附量为653 mg/g;IgY-NHA在36 h内,IgY蛋白得到缓慢持续的释放。最终释放百分比为34%;IgY-NHA对Pg的最小抑菌浓度为6 g/L,IgY-NHA可延迟Pg进入对数生长期的时间点,并能够抑制Pg生长,且具有浓度依赖性。结论: 纳米羟基磷灰石对卵黄抗体具有良好的吸附能力及缓释性能,且IgY-NHA能够抑制Pg的增殖。

关键词: 纳米羟基磷灰石, 卵黄抗体, 复合材料, 牙龈卟啉单胞菌

Abstract: Objective: To prepare nano-hydroxyapatite composite with the sustained release function of egg yolk antibody and test in vitro antibacterial properties. Methods: The morphology and particle size of nano-hydroxyapatite (nano-hydroxyapatite, NHA) were observed by scanning electron microscopy (SEM). The nano-hydroxyapatite was loaded with the anti-porphyromonas gingivalis (anti-P. gingival-IgY) and the drug release behavior of the IgY-NHA composite was studied. Porphyromonas gingival (Pg) was selected as the experimental strain to study the minimal inhibitory concentration (MIC) of IgY-NHA composite. The growth inhibitory effect of IgY-NHA composite on P.gingival was studied to evaluate the antibacterial properties of delayed release of IgY nano-hydroxyapatite. Results: NHA was a porous structure composed of granular nanostructures. With the increase of loading concentration of IgY, the adsorption capacity of NHA increased significantly. When the loading concentration of IgY was 4 g/L, the adsorption capacity was 653 mg/g. Within 36 h, IgY protein was released slowly and continuously. The final release percentage was 34%, the minimum inhibitory concentration of IgY-NHA to P.gingival was 6 g/L. IgY-NHA could delay the time point of P.gingival entering the logarithmic growth period and inhibit P.gingival growth, which was concentration dependent. Conclusion: Nano-hydroxyapatite has good adsorption and sustained-release properties for yolk antibody, and IgY-NHA can inhibit the proliferation of P. gingival.

Key words: nano-hydroxyapatite, yolk antibody, composite materials, porphyromonas gingival