常用分子检测技术在儿童龋病微生物多样性研究中的应用

doi:10.13701/j.cnki.kqyxyj.2016.02.025

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (2): 202-204.DOI: 10.13701/j.cnki.kqyxyj.2016.02.025

• 综述 • 上一篇

常用分子检测技术在儿童龋病微生物多样性研究中的应用

朱玉娟¹ 焦康礼¹ 周建业² 余占海¹ 李志强^2*

1. 兰州大学口腔医学院修复科甘肃兰州 730000;
2. 西北民族大学口腔医学国家民委重点实验室甘肃兰州 730030

收稿日期:2015-07-01 出版日期:2016-02-28 发布日期:2016-03-10
通讯作者: 李志强,E-mail:lizhiqiang6767@163.com
作者简介:朱玉娟(1987~),女,山东人,硕士,主要从事口腔微生物群落结构多样性以及口腔修复临床研究。
基金资助:
国家自然科学基金地区基金项目(编号:31360124/C0309)
甘肃省科技厅,甘肃省科技支撑项目(编号:1204FKCA168)

Applications of Common Molecular Detection Techniques in the Study of Microbial Diversity in Dental Caries among Children.

ZHU Yu-juan¹, JIAO Kang-li¹, ZHOU Jian-ye², YU Zhan-hai¹, LI Zhi-qiang².

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, China

Received:2015-07-01 Online:2016-02-28 Published:2016-03-10

摘要/Abstract

摘要： 龋病是口内常见的慢性、感染性疾病,在儿童中的发病率呈上升趋势。微生物是引起龋病的主要因素之一,对于致龋微生物的种类、致龋机制及其多样性是目前口腔微生物学的研究热点。随着现代分子生物学技术的不断发展,分子检测技术被广泛用于微生物的研究中,早期检测及预防对降低儿童龋病发病率有重要作用。本综述着重论述常用分子检测技术在儿童龋病微生物多样性研究方面的应用,并对未来儿童口腔微生物多样性方面的研究进行展望。

关键词: 微生物, 龋病, 儿童, 16SrDNA, 分子检测

Abstract: Dental caries is one of the most common chronic infectious diseases worldwide, and the incidence in children is increasing. Microorganisms are one of the main factors that cause dental caries. The species, mechanisms, and diversity of cariogenic microorganisms are hot topics in oral microbiology research. With the development of modern molecular biological techniques, molecular biological detection techniques are now widely used in microbiological research. These early detection and prevention methods play an important role in reducing the incidence of caries disease in children. This review focuses on the research advancement of some modern molecular biotechnology in oral microbial diversity research. In addition, it also made a prospect for the study of oral microbial diversity in children.

中图分类号:

R781.1

朱玉娟,焦康礼,周建业,余占海,李志强. 常用分子检测技术在儿童龋病微生物多样性研究中的应用[J]. 口腔医学研究, 2016, 32(2): 202-204.

ZHU Yu-juan, JIAO Kang-li, ZHOU Jian-ye, YU Zhan-hai, LI Zhi-qiang.. Applications of Common Molecular Detection Techniques in the Study of Microbial Diversity in Dental Caries among Children.[J]. Journal of Oral Science Research, 2016, 32(2): 202-204.

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常用分子检测技术在儿童龋病微生物多样性研究中的应用

Applications of Common Molecular Detection Techniques in the Study of Microbial Diversity in Dental Caries among Children.

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