口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 55-58.DOI: 10.11839/kqyxzh.2016.01.014

• ·临床研究论著· • 上一篇    下一篇

高龋无龋儿童乳牙牙菌斑的微生物差异

冯岩,严恒,薛凯佳,卢友光*   

  1. 福建医科大学附属口腔医院预防科 福建 福州 350002
  • 收稿日期:2015-05-29 出版日期:2016-01-28 发布日期:2016-03-21
  • 通讯作者: 卢友光,E-mail:fjlyg63@163.com
  • 作者简介:冯岩(1976~),女,河北辛集人,博士,副主任医师,主要从事口腔预防医学研究工作。
  • 基金资助:
    福建省自然科学基金(编号:2014J01317)

Microbial Differences in Dental Plaque of Deciduous Teeth between Caries-active Children and Caries-free Children.

FENG Yan, YAN Heng, XUE Kai-jia, LU You-guang.   

  1. Department of Preventive Dentistry, The Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China
  • Received:2015-05-29 Online:2016-01-28 Published:2016-03-21

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摘要： 目的:探讨高龋儿童和无龋儿童的乳牙牙菌斑中微生物种类的差异。方法:选取符合WHO取样标本的3名高龋儿童和3名无龋儿童,每个高龋儿童取唇颊侧、舌侧和病损区菌斑3份,每个无龋儿童取唇颊侧和舌侧2份,提取全基因组DNA,行16SrDNA V1-V2区PCR扩增并构建文库,454焦磷酸测序。所得序列进行一系列处理后利用RDP分类器进行分类,并进行统计分析,比较高龋无龋儿童乳牙牙菌斑的微生物的不同。结果:牙菌斑的15份样本共得到324262条序列,每个样本平均约含有21617条序列,每条序列平均约长268bp;在高龋儿童中,唇颊侧组、舌侧组以及病损区组3组之间比较差异具有统计学意义(P＜0.05)的5个菌属是链球菌属(Streptococcus)、丙酸杆菌属(Propionibacterium)、棒状杆菌属(Corynebacterium)、纤毛菌属(Leptotrichia)、乳杆菌属(Lactobacillus);高龋组与无龋组比较差异具有统计学意义(P＜0.05)的5个菌属是链球菌属(Streptococcus)、栖热菌属(Thermus)、产丝菌属(Filifactor)、Solobacterium、短芽孢杆菌属(Brevibacillus)。结论:牙菌斑微生物的检出率在高龋和无龋儿童中存在差异。

关键词: 龋病, 乳牙, 牙菌斑, 微生物, 16SrDNA

Abstract: Objevtive: To discuss the microbial differences in dental plaque of deciduous teeth between caries-active children and caries-free children. Methods: Three caries-active children and three caries-free children were selected according to WHO standard. Three specimens of plaque (from labial-buccal surfaces, lingual surfaces and caries lesions) were taken from each caries-active children and two specimens of plaque (from labial-buccal surfaces and lingual surfaces) were taken from each caries-free children. DNA of the whole genome was extracted. PCR amplification of the V1-V2 region of 16SrDNA was conducted and the gene library was constructed. The method of 454 pyrosequencing was used. The resultant sequences were classified by RDP classifier and the statistic analysis was conducted. Results: A total of 324,262 gene sequences were obtained from 15 specimens, there were 21617 gene sequences for each specimen averagely and the average length was 268 bps. In caries-active children, 5 bacterial strains were found significantly different among the different locations (P<0.05). They were Streptococcus, Propionibacterium, Corynebacterium, Leptotrichia and Lactobacillus. There were 5 bacterial strains significantly different between caries-active children and caries-free children (P<0.05), including Streptococcus, Filifactor, Solobacterium, Thermus and Brevibacillus. Conclusion: The microorganism detection rate in dental plaque is different between caries-active and caries-free children.

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