加速成骨正畸(AOO)过程中破骨细胞的形成情况

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (3): 205-208.

• 基础研究论著 • 下一篇

加速成骨正畸(AOO)过程中破骨细胞的形成情况

陈雅文^1,2, 高龙华^1,2, 江久汇^1*, 李翠英^1*, 王海丞¹, 刘畅^1,3, 许舒宇^1,3, 杨月¹, 彭靖¹

1. 北京大学口腔医学院正畸科北京 100081;
2. 大连医科大学;
3. 山东大学口腔医学院

收稿日期:2014-10-23 出版日期:2015-03-28 发布日期:2016-04-29
通讯作者: 江久汇,E-mail:drjiangw@163.com
李翠英,E-mail:licuiying_67@163.com
作者简介:陈雅文(1987~ ),女,浙江人,硕士,住院医师,从事口腔正畸学临床及基础研究,现在嘉兴市第一医院工作。
基金资助:
国家自然科学基金(编号:303075816);国家自然科学基金(编号:81072214);北京市科学技术委员会首都临床特色应用研究基金(编号:303130219)

Differentiation of Osteoclasts in the Process of Accelerated Osteogenic Orthodontic.

CHEN Ya-wen, GAO Long-hua, JIANG Jiu-hui.

The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081

Received:2014-10-23 Online:2015-03-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:加速成骨正畸能增加骨改建及牙移动的速率,但仅仅只有部分关于加速成骨正畸的机制被获知。本实验进一步探讨骨改建过程中破骨细胞的分化形成情况,以更好地了解加速成骨正畸的机制。方法:40只新西兰大白兔随机分成5组,每组8只。下颌左侧作为加速成骨正畸实验侧,右边作为常规正畸对照侧。左右两侧均用4盎司的力值。在1、3、5、7、14 d将实验动物处死。每个时间点上的3只动物用作形态学观察,另外5只用作分子生物研究。结果:形态学观察显示,加速成骨实验组压力侧破骨细胞计数及骨改建活跃程度均较对照组高,而且有2次连续明显的增长。在加速成骨正畸(AOO)中,可观察到破骨细胞不同的分化因子在同一时期表达趋势不同:在第5天,实验组,CTSK,TRAP的mRNA显著上调并达到高峰;CTR的mRNA表达量在1~7 d较平缓;JDP2、NFATC1的mRNA的表达量趋势相一致,在第7天达到高峰;Fra2的mRNA在第5天开始增加,之后持续升高。结论:提示在AOO过程中,破骨细胞可能有2次明显的连续分化,继而能持续的加速牙齿移动。

关键词: 牙移动, 加速成骨正畸, 骨改建, 因子, 破骨细胞

Abstract: Objective: To optimize alveolar decortications-facilitated orthodontic and to study the mechanism underlying . Methods: In this study, 40 rabbits were randomly divided into 5 groups of 8 rabbits each. The left side of the mandible served as the experimental side, which was treated by alveolar decortication-facilitated orthodontic and the right side served as the control side. 4 oz force was applied to the mandibular first molar. The measurements on 8 rabbits were done on days 1, 3, 5, 7 and 14. Three of the animals at each time point were used for histological observation. The other five animals were used for molecular studies. Results: Histological observation demonstrated a higher count of osteoclasts and bone remodeling activity and two consecutive obvious growth in the experimental group. In addition, different cytokines of the osteoclast had different expression trend at the same time: the mRNA expression of CTSK and TRAP was markedly upregulated and reached its highest on the 5th day; the CTR mRNA was expressed evenly on day 1 through day 7, and the trend of JDP2 and NFATC1 expression was consistent, reaching its highest on the 7th day; the Fra2 expression increased on the 5th day and was significantly higher on days 5, 7, and 14 days. Conclusion: It suggests that among this procedure osteoclast may differentiate twice to accelerate roots movement.

Key words: Tooth movement, Accelerate osteogenic orthodontic , Bove remodeling , Cytokines , Osteo clasts

中图分类号:

R783.5

陈雅文, 高龙华, 江久汇, 李翠英, 王海丞, 刘畅, 许舒宇, 杨月, 彭靖. 加速成骨正畸(AOO)过程中破骨细胞的形成情况[J]. 口腔医学研究, 2015, 31(3): 205-208.

CHEN Ya-wen, GAO Long-hua, JIANG Jiu-hui.. Differentiation of Osteoclasts in the Process of Accelerated Osteogenic Orthodontic.[J]. Journal of Oral Science Research, 2015, 31(3): 205-208.

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加速成骨正畸(AOO)过程中破骨细胞的形成情况

Differentiation of Osteoclasts in the Process of Accelerated Osteogenic Orthodontic.

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