口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (4): 336-339.

• 基础研究论著 • 上一篇    下一篇

PRF复合自体骨髓间充质干细胞修复兔牙槽骨缺损的研究

张文丽, 李淑慧, 陈诚, 李一鸣, 周晶, 吴佩玲*   

  1. 新疆医科大学第二附属医院口腔科 新疆 乌鲁木齐 830063)
  • 收稿日期:2014-09-01 出版日期:2015-04-28 发布日期:2016-04-29
  • 通讯作者: 吴佩玲,Email: wplkq@sina.com
  • 作者简介:张文丽(1988~ )女,新疆人,硕士,主要从事口腔种植与修复的基础研究工作。
  • 基金资助:
    新疆维吾尔自治区青年科学基金项目(编号:2013211B53)

Study of PRF Combined with Autogenous Bone Marrow Stem Cells on Alveolar Bone Defect.

ZHANG Wen-li, LI Shu-hui, CHEN Cheng, et al   

  1. Department of Stomatology, The Second Affiliated Hospital to Xinjiang Medical University, Urumqi 830063
  • Received:2014-09-01 Online:2015-04-28 Published:2016-04-29

摘要： 目的:观察富血小板纤维蛋白(platelet-rich fibrin, PRF)复合自体第3代骨髓间充质干细胞(bone marrow stem cells, BMSCs)修复牙槽骨缺损的能力。方法:取健康雄性2月龄新西兰兔36只,随机分为4组:PRF+BMSCs组、PRF组、BMSCs组、空白对照组,均在全麻下微创拔除下颌左侧中切牙。4组实验动物分别在4、8、12周处死。取其下颌骨进行牙槽骨标本测量,X线观察,组织学观察。结果:牙槽骨标本测量比较:4组牙槽嵴宽度、高度丧失值均存在差异(P＜0.05);析因分析示PRF与BMSCs复合使用修复牙槽骨的效果优于单独使用PRF或BMSCs(P<0.05)。X线、组织学观察表明术后4、8、12周时拔牙窝骨吸收程度:PRF+BMSCs组＜PRF组＜BMSCs组＜空白对照组,PRF+BMSCs组成骨情况均优于PRF组、BMSCs组、空白对照组。结论:PRF复合自体BMSCs可以促进牙槽骨的再生与修复,具有潜在的临床应用价值。

关键词: 骨髓间充质干细胞, 富血小板纤维蛋白, 牙槽骨缺损

Abstract: Objective: To observe the bone formation of platelet-rich fibrin(PRF) combined with autogenous bone marrow stem cells(BMSCs) in rabbits alveolar bone defects. Methods: Thirty-six male 2 months rabbits were divided into four groups randomly: PRF+BMSCs, PRF, BMSCs, blank control. They were minimally invasive removal of mandible incisor under general anesthesia on the left side. After 4 weeks,8 weeks,12 weeks,36 rabbits were sacarificed by injecting air to vein. Then the jaws were investigated by general observation, X-ray photography and structual observation. Results: There were significant difference in alveolar ridge width and height among four groups via measuring alveolar bone specimen.The effect of repairing alveolar bone in BMSCs+PRF was better than using BMSCs or PRF alone in factorial analysis(P＜0.05). X-ray and histological observation showed that the degree of bone resorption was observed as follows: PRF+BMSCs<PRF<BMSCs<blank control(P＜0.05)and the bone formation of PRF+BMSCs was better than PRF, BMSCs and blank control after 4 weeks,8 weeks and 12 weeks. Conclusion: PRF composited BMSCs can promote alveolar bone regeneration, repairation and had potential clinical application value.

Key words: Bone marrow stem cells , Platelet-rich fibrin, Alveolar bone defect

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