口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (5): 429-432.

• 基础研究论著 •    下一篇

纯镁微弧氧化-HF-硅溶胶复合处理涂层细胞相容性研究

刘继光, 孙玉龙, 李慕勤*, 刘江, 李晓光   

  1. 刘继光 孙玉龙 李慕勤* 刘江 李晓光(佳木斯大学 黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007
  • 收稿日期:2014-10-28 出版日期:2015-05-28 发布日期:2016-04-29
  • 通讯作者: 李慕勤,E-mail:jmsdxlimuqin@163.com
  • 作者简介:刘继光(1961~ ),男,汉族,黑龙江人,教授,主要从事生物医学材料的研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:31370979);黑龙江省高校创新团队建设计划项目(编号:2012TD010)

Biocompatibility of Pure Magnesium Ultrasonic Micro-arc Oxidation-HF-Silica Sol Compounded Treatment.

LIU Ji-guang, SUN Yu-long, LI Mu-qin, et al.   

  1. Key Laboratory of Biomedical Materials of Heilongjiang Province Colleges,Jiamusi University,Jiamusi 154007
  • Received:2014-10-28 Online:2015-05-28 Published:2016-04-29

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摘要： 目的:对纯镁微弧氧化经HF-硅溶胶复合处理涂层进行成骨细胞生物相容性检测,评价纯镁表面微弧氧化经不同复合处理涂层后的细胞相容性。方法:实验分为3组,纯镁微弧氧化组为对照组(A组)、纯镁微弧氧化-硅溶胶复合处理组(B组)、纯镁微弧氧化-HF-硅溶胶复合处理组(C组)。将MC3T3-E1系成骨细胞接种在3组材料表面,分别通过扫描电镜、激光共聚焦、CCK-8、ALP对成骨细胞的生长、黏附、增殖以及活性进行检测。结果:扫描电镜和激光共聚焦显示B组和C组表面细胞的生长和黏附均优于A组,C组优于B组。CCK-8和ALP的检测结果显示3组材料表面细胞的增殖、活性为C组＞B组＞A组,3组的比较差异均具有统计学意义。结论:3组材料均具有良好的生物相容性,而纯镁微弧氧化-HF-硅溶胶复合处理后的生物相容性最佳。

关键词: 纯镁, 微弧氧化, HF, 硅溶胶, 成骨细胞

Abstract: Objective: To study the biocompatibility of pure magnesium micro-arc oxidation-HF-silica sol composite treatment, and to evaluate the biocompatibility of pure magnesium micro-arc oxidation after different composite treatment. Methods: The experiment was divided into three groups, the pure magnesium micro-arc oxidation was used as the control group (group A). The pure magnesium micro-arc oxidation-silica sol composite treatment was group (group B). The pure magnesium micro-arc oxidation-HF-silica sol composite treatment was group (group C). MC3T3-E1 osteoblasts were inoculated on material surface of three groups, by scanning electron microscopy (SEM), laser confocal scanning microscope, CCK-8, ALP to test respectively growth, adhesion, proliferation and activity of osteoblasts. Results: The scanning electron microscope and laser confocal scanning microscope demonstrated that on cell growth and adhesion group B and group C surface were superior to group A. Group C was better than that of group B. The results of CCK-8 and ALP detection showed that the proliferation and activity of material surface of three groups were group C > group B > group A. The comparative differences of three groups were statistically significant. Conclusion: The pure magnesium micro-arc oxidation silicon sol composite treatment and pure magnesium micro-arc oxidation-HF-silicon sol composite treatment had all good biocompatibility, and the biocompatibility of HF-silica sol composite treatment was the best.

Key words: Pure magnesium , Micro-arc oxidation, Hydrogen fluoride , Silica sol, Osteoblast

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