基质金属蛋白酶-8和基质金属蛋白酶-13在人根尖周病组织肥大细胞上的表达

doi:10.13701/j.cnki.kqyxyj.2016.06.013

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (6): 605-609.DOI: 10.13701/j.cnki.kqyxyj.2016.06.013

基质金属蛋白酶-8和基质金属蛋白酶-13在人根尖周病组织肥大细胞上的表达

范挽亭^1,2 ,张铮¹ ,李娟² ,黄世光^2*

1. 暨南大学第二临床医学院·深圳市人民医院口腔医学中心广东深圳 518020;
2. 暨南大学医学院口腔医学系广东广州 510632

收稿日期:2016-01-19 出版日期:2016-06-26 发布日期:2016-06-22
通讯作者: 黄世光,电话:020-85221165
作者简介:范挽亭(1983~ ),男,广东人,主治医师,硕士在读,主要从事根尖周病研究工作。
基金资助:
广东省社会发展领域科技计划项资助项目(编号:2013B021800043,2014A020212212)

Expression of MMP-8 and MMP-13 on Mast Cells in Human Chronic Apical Periodontitis

FAN Wan-ting^1,2, ZHANG zheng¹, LI Juan², HUANG Shi-guang^2*.

1. Oral Medical Center, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen 518020, China;
2. Faculty of Dentistry, School of Medicine, Jinan University, Guangzhou 510632, China.

Received:2016-01-19 Online:2016-06-26 Published:2016-06-22

摘要/Abstract

摘要： 目的:观察基质金属蛋白酶-8(matrixmetalloproteinase-8,MMP-8)和基质金属蛋白酶-13(matrixmetalloproteinase-13,MMP-13)在人慢性根尖周炎组织肥大细胞(mastcells,MCs)上的表达情况,探讨肥大细胞在根尖周病发病机制中的作用。方法:将90例样本分为3组:1)根尖肉芽肿组30例;2)根尖囊肿组30例;3)正常牙周膜组30例。组织标本置于10%中性福尔马林固定液中浸泡48h以上,制作连续组织切片。HE染色,光学显微镜下观察各组的组织学变化;免疫荧光双染色(doubleimmunofluorescence,DIF),荧光显微镜下观察MMP-8和MMP-13在根尖周组织MCs上表达的情况。结果:与正常对照组相比,两组慢性根尖周炎组织中MMP-8和MMP-13双阳性MCs数目均显著增多(P<0.01);根尖囊肿组与根尖肉芽肿组MMP-8和MMP-13双阳性MCs密度无显著性差异。结论:慢性根尖周炎病变组织中MCs数目明显增多,同时MMP-8和MMP-13双阳性MCs密度显著增高,提示MMP-8和MMP-13阳性MCs可能在慢性根尖周炎的致病机制中发挥作用。

关键词: 慢性根尖周病, 肥大细胞, 基质金属蛋白酶

Abstract: Objective: To identify and quantify the expression of MMP-8 and MMP-13 on mast cells (MCs) in different types of human chronic apical periodontitis tissues using double immunofluorescence staining. Mehtods: The specimens (n=90), including healthy control (n=30), periapical cyst (n=30) and periapical granuloma (n=30), were involved in the present study. The tissue samples were fixed in 10% buffered formalin for at least 48 hours and then embedded in paraffin. Serial 5-μm-thick sections were deposited onto SuperFrost/Plus microscope glasses. Routine staining of the sections using hematoxylin & eosin (HE) was performed for morphological evaluation. The number of MMP-8 and MMP-13 positive MCs was identified by double immunofluorescence staining. Results: The density of MMP-8 and MMP-13 positive MCs in the periapical lesions was obviously higher than that in the healthy controls (P<0.01). However, no significant difference of the density of MMP-8 and MMP-13 positive MCs between periapical cyst and periapical granuloma was observed. Conclusion: There is significantly increased number of MCs, along with increased density of MMP-8 and MMP-13 positive MCs in human periapical lesions, which suggests that MMP-8 and MMP-13 positive MCs may play an important role in the pathogenesis of human periapical diseases.

中图分类号:

R781.4

范挽亭 ,张铮 ,李娟 ,黄世光. 基质金属蛋白酶-8和基质金属蛋白酶-13在人根尖周病组织肥大细胞上的表达[J]. 口腔医学研究, 2016, 32(6): 605-609.

FAN Wan-ting, ZHANG zheng, LI Juan, HUANG Shi-guang.. Expression of MMP-8 and MMP-13 on Mast Cells in Human Chronic Apical Periodontitis[J]. Journal of Oral Science Research, 2016, 32(6): 605-609.

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基质金属蛋白酶-8和基质金属蛋白酶-13在人根尖周病组织肥大细胞上的表达

Expression of MMP-8 and MMP-13 on Mast Cells in Human Chronic Apical Periodontitis

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