富血小板纤维蛋白修复犬股骨髁骨缺损的微观结构研究

doi:10.13701/j.cnki.kqyxyj.2016.08.005

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (8): 794-799.DOI: 10.13701/j.cnki.kqyxyj.2016.08.005

富血小板纤维蛋白修复犬股骨髁骨缺损的微观结构研究

肖琼¹,董露²,杨琴秋³,孙勇^1*,陈红亮¹,赵峰¹,芦帅¹

1. 中国人民解放军成都军区机关医院口腔科四川成都 610041;
2. 凉山彝族自治州第一人民医院口腔科四川西昌 615000;
3. 遂宁市第一人民医院口腔科四川遂宁 629000

收稿日期:2016-01-06 出版日期:2016-08-26 发布日期:2016-08-26
通讯作者: 孙勇,电话:13808008089
作者简介:肖琼(1987~ ),女,重庆市沙坪坝区人,硕士,医师,主要从事口腔种植义齿修复的基础与临床研究。
基金资助:
全军"十二五"科研面上项目(CWS11J024);军事口腔医学国家重点实验室开放课题(2014KB04)

Microstructure Studies on Platelet-rich Fibrin Repairing Bone Defects in the Beagle Dog

XIAO Qiong¹, DONG Lu², YANG Qin-qiu³,SUN Yong^1*, CHEN Hong-liang¹, ZHAO Feng¹, LU Shuai¹

1. Department of Stomatology, Chengdu Military Authorities Hospital of Chinese People's Liberation Army, Chengdu 610041,China;
2. Department of Stomatology, The First People's Hospital of Liangshan Yi Autonomous Prefecture, Xichang 615000,China;
3. Department of Stomatology, the First People's Hospital of Suining, Suining 629000, China.

Received:2016-01-06 Online:2016-08-26 Published:2016-08-26

摘要/Abstract

摘要： 目的:通过建立beagle犬股骨髁的骨缺损模型,观察富血小板纤维蛋白(platelet-rich fibrin,PRF)诱导骨再生过程中新生骨的微观结构,评价PRF修复骨缺损的特点和优势,为PRF在诱导骨再生及颌骨重建的临床应用提供新的实验依据。方法:在实验犬股骨髁制备骨缺损,按Choukroun方法制备PRF膜,将PRF膜、Bio-Oss骨替代材料及自体骨骨松质随机填入3处骨缺损。12周、24周处死动物并取材,采用微焦点计算机断层摄影系统(microfocus computerizedtomography system,Micro-CT)检测分析新生骨的几何信息和结构信息。结果:PRF新生诱导骨骨小梁结构呈板状,厚度较厚,连续性和成熟度良好,数量稍少,接近自体骨组的成骨情况,优于Bio-Oss组;观察期内均数随时间延长,有向天然骨组衍变的趋势;PRF诱导成骨表现为向心性的特点。结论:PRF可诱导新生骨形成,成骨效果接近生理状态,表现为向心性成骨方式,具有良好的应用前景。

关键词: 富血小板纤维蛋白, 骨缺损, 骨诱导, 微焦点计算机断层摄影系统

Abstract: Objective: To observe the microstructure of new bone formed from PRF induction and evaluate the features and advantages of PRF on bone repair by the femoral condyle defect model inbeagle dogs, providingnew experimental basis forosteanagenesis and mandibular reconstruction. Methods: The PRF membrane was prepared by means of Choukroun. PRF, Bio-Oss bone substitute materials and autologous cancellous bone were randomly filled into bone defects in the femoral condyle of dog. At 12 weeks and 24 weeks,the dogswere sacrificedand the bone was retrieved. Micro-CT was used to detect and analyze geometry and structure information of new bone. Results: New bone formedfrom PRF hadplate-shape and thicker trabecular structure with good continuity and maturity, which was highly close to that inthe autogenous bone group and better than that in the Bio-Oss group. During the observation period, the average values were closeto natural bone. In bone regeneration process, PRF osteoinduction showed concentric features. Conclusion: PRF could induce the formation of new bone, microstructures of which were similar to physiological conditions and showed central regeneration, thus showing a prospect for clinical application.

Key words: Platelet-rich fibrin, Bone defects, Osteoinduction, Micro-CT

中图分类号:

R782.1

肖琼,董露,杨琴秋,孙勇,陈红亮,赵峰,芦帅. 富血小板纤维蛋白修复犬股骨髁骨缺损的微观结构研究[J]. 口腔医学研究, 2016, 32(8): 794-799.

XIAO Qiong, DONG Lu, YANG Qin-qiu,SUN Yong, CHEN Hong-liang, ZHAO Feng, LU Shuai. Microstructure Studies on Platelet-rich Fibrin Repairing Bone Defects in the Beagle Dog[J]. Journal of Oral Science Research, 2016, 32(8): 794-799.

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富血小板纤维蛋白修复犬股骨髁骨缺损的微观结构研究

Microstructure Studies on Platelet-rich Fibrin Repairing Bone Defects in the Beagle Dog

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