口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (8): 800-803.DOI: 10.13701/j.cnki.kqyxyj.2016.08.006

• 基础研究论著 • 上一篇    下一篇

口腔鳞癌细胞分泌的胞外体对其生物学行为的影响

李宠1,朱杨1,山本哲也2,李德超1*   

  1. 1. 佳木斯大学附属口腔医院颌面外科 黑龙江 佳木斯 154007;
    2. 高知医科大学口腔颌面外科 高知,783-8505
  • 收稿日期:2015-12-11 出版日期:2016-08-26 发布日期:2016-08-26
  • 通讯作者: 李德超,E-mail:dechaoli2004@163.com
  • 作者简介:李宠(1988~ ),女,辽宁锦县,硕士在读,主要从事口腔颌面外科的基础研究。
  • 基金资助:
    黑龙江省卫生厅课题(项目编号:2013-218)

Effects of Exosomes Secreted by Oral Squamous Cell Carcinoma Cells on Their Biological Behaviors

LI Chong1, ZHU Yang1, YAMAMOTO Tetsuya2, LI De-chao1*   

  1. 1. Department of Oral and Maxillofacial Surgery, School of Stomatology, Jiamusi University, Jiamusi 154007,China;
    2. Department of Oral and Maxillofacial Surgery, Kochi University, Kochi 783-8505, Japan.
  • Received:2015-12-11 Online:2016-08-26 Published:2016-08-26

摘要： 目的:研究口腔鳞状细胞癌细胞细胞株(OSC-4)分泌的胞外体对细胞生物学行为的影响。方法:培养 OSC-4,从其上清培养液中分离提纯胞外体(exosome, EXO),CD9标记,将提取的胞外体作用于OSC-4,MTT检测胞外体对 OSC-4增殖能力的影响,细胞划痕实验检测胞外体对OSC-4迁移能力的影响,transwill小室侵袭实验检测胞外体对OSC-4侵袭能力的影响。结果:细胞上清液提取物免疫金标结果可见金标记的CD9,即分离提纯物为胞外体;MTT检测结果显示胞外体作用后的OSC-4的增殖能力增强(P＜0.05),细胞划痕实验结果显示其迁移能力增强和transwill小室侵袭实验结果显示其侵袭能力也增强(P＜0.05)。结论:OSC-4分泌的胞外体可以促进口腔鳞状细胞癌细胞的增殖、迁移、浸润能力。

关键词: 口腔鳞状细胞癌, 胞外体, 生物学行为

Abstract: Objective: To study the effectsof exosomes secreted byoral squamous cell carcinoma cells (OSC-4) on their biological behaviors. Methods: Exosomesderived from OSC-4 cells were separated from the medium. The effects of exosomes on the OSC-4 cell proliferation, migration and invasion were detected by MTT, wound healing, andtranswellassays, respectively. Results: Gold-labeling CD9 was detected in the isolated exosomes. MTT assay showed that the OSC-4 cells proliferation ability was increased after treated with exosomes(P<0.05). Wound healing and transwell assays results showed that the migration and invasion ability of OSC-4 cells were also increased after treated byexosomes (P<0.05). Conclusion: Exosomes secreted byoral squamous cell carcinomacells couldpromote their proliferation, migration, and invasion.

Key words: Oral squamous cell carcinoma, Exosome, Biological behavior

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