Preparation of Naturally Derived Acellular Bovine Pericardium for Guiding Bone Regeneration.

doi:10.13701/j.cnki.kqyxyj.2016.05.004

Journal of Oral Science Research ›› 2016, Vol. 32 ›› Issue (5): 440-444.DOI: 10.13701/j.cnki.kqyxyj.2016.05.004

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Preparation of Naturally Derived Acellular Bovine Pericardium for Guiding Bone Regeneration.

BAI Ming-hai^1*, ZHANG Ting-ting², XIE Hui³, WU Han-jiang⁴, ZHAO Zhi-he⁵, HE Zheng-quan¹, LIU Xin-yu¹.

1. Department of Orthodontics, Changsha Stomatology Hospital, Changsha 410005, China;
2. Department of Laboratory, Xiangya Second Hospital. Central South University, Changsha 410011, China;
3.Department of Periodontology,ChangshaStomatology Hospital, Changsha 410005,China;
4. Department of Stomatology, Xiangya Second Hospital, Central South University, Changsha 410011, China;
5. West China College of Stomatology, Sichuan University, Chengdu 610041, China.

Received:2016-01-11 Online:2016-05-26 Published:2016-05-26

Abstract

Abstract: Objective: To find out the best way for decellularization and cross-linking of naturally derived bovine pericardiumto prepare GBR membrane which can meet the clinical demand. Methods: Natural bovine pericardium were decellularized using trypsin+ detergent method (I), trypsin +enzymatic nucleic acid method (II), trypsin+ detergent +enzymatic nucleic acid method (III), freeze- thaw+detergent method (IV), freeze-thaw + enzymatic nucleic acid method (V), or freeze- thaw+detergent + enzymatic nucleic acid method (VI), and then were cross-linked using glutaraldehyde or genipin. The best way of decellularization and cross-linked method were screened by means of optical microscope, scanning electron microscope, cell toxicity test, determination of the mechanical properties, and measurement of the chemotactic properties. Results: Freeze- thaw+detergent + enzymatic nucleic acid method with bovine pericardium was the best method of decellularization. Genipin crosslinking method by which acellular bovine pericardium was crosslinked was better. Conclusion: Freeze- thaw+detergent + enzymatic nucleic acid method and genipin crosslinking method were ideal method for preparation of natural bovine pericardium.

CLC Number:

R318.08

BAI Ming-hai, ZHANG Ting-ting, XIE Hui, WU Han-jiang, ZHAO Zhi-he, HE Zheng-quan, LIU Xin-yu.. Preparation of Naturally Derived Acellular Bovine Pericardium for Guiding Bone Regeneration.[J]. Journal of Oral Science Research, 2016, 32(5): 440-444.

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Preparation of Naturally Derived Acellular Bovine Pericardium for Guiding Bone Regeneration.

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