Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens.

doi:10.13701/j.cnki.kqyxyj.2016.10.010

Abstract

Abstract: Objective: To evaluate whether the oral administration of Lactobacillus salivarius w22a could alleviate the peri-implantitis pathogens in subgingival plaque, such as Porphyromonas gingivalis (Pg), Pevotella intermedia (Pi), and Fusobacteriurn nuclearum (Fn) in vitro.Methods: The antagonistic growth effects of Lactobacillus salivarius as well as their probiotic on periodontal pathogens, including Pg, Pi and Fn, were tested using drug resistance experiment, agglutination experiments, and antibacterial experiments.Results: The results revealed that Lactobacillus salivarius w22a could produce antibiotics such as organic acid, hydrogen peroxide and EPS. Strain w22a could resist lysozyme and antibiotics at certain concentration. In addition, auto-agglutination and agglutination with other pathogens could be observed. The fermentation of w22a inhibited the growth of pathogens to varied degrees.Conclusion: Lactobacillus salivarius w22a was a potential probiotic strain to inhibit the growth of Pg, Pi and Fn in vitro.

Key words: Lactobacillus salivarius, Probiotic, Peri-implantitis

CLC Number:

R781.3

XUE Long, WANG Li-he, NIE Hong-bing, ZHOU Hai-jing, GUO Man-li, KANG Yong-jie, YANG Lan.. Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens.[J]. Journal of Oral Science Research, 2016, 32(10): 1042-1046.

References

[1] Simonis P, Dufour T, Tenenbaum H. Long-term implant survival and success: a 10-16-year follow-up of non-submerged dental implants[J].Clin Oral Implants Res,2010, 21(7)∶772-777
[2] Dhir S.Biofilm and dental implant: The microbial link.[J].J Indian Soc Periodontol,2013,17(1)∶5-11
[3] Seneviratne CJ, Zhang CF, Samaranayake LP. Dental plaque biofilm in oral health and disease[J]. Chin J Dent Res, 2011, 14(2)∶87-94
[4] Quirynen M,Vogels R,Peeters W eta1. Dynamics of initial subgingival colonization of'pristine peri-implant pockets[J]. ClinOral Implants Res,2006,17(1)∶25-37
[5] Mombelli A,Schmid B, Rutar A, et al. Local antibiotic therapy guided by microbiological diagnosis[J].Clin Periodontol,2002,29(8)∶743- 749
[6] Maruyama N, Maruyama F, Takeuchi Y, et al. Intraindividual variation in core microbiota in peri-implantitis and periodontitis[J]. Scientific Reports, 2014, 4∶6602-6602
[7] Dhir S.Biofilm and dental implant: The microbial link.[J].Indian Soc Periodontol, 2013, 17(1):5-11
[8] Assche N,Van Essche M,Pauwels M,et aL. Do periodontopathogens disappear after full-mouth tooth extraction[J]. J Clin Periodontol,2009,36(12)∶1043-1047
[9] Chrcanovic BR. A History of Periodontitis Suggests a Higher Risk for Implant Loss [J]. Evid Based Dent Pract, 2015, 15(4)∶185-186
[10] Valderrama P, Blansett JA, Gonzalez MG, et al. Detoxification of Implant Surfaces Affected by Peri-Implant Disease: An Overview of Non-surgical Methods [J]. Open Dent J, 2014, 16(8)∶77-84
[11] 李璐,陆峻君.益生菌对口腔微生物的影响[J].中国微生态学杂志,2010,01∶92-93+97
[12] Garima Jindala, Ramesh Kumar Pandeyb, Rajeev Kumar Singhc, et al. Can early exposure to probiotics in children prevent dental caries? A current perspective [J]. Journal of Oral Biology and Craniofacial Research.Volume 2, Issue 2, May-August, 2012, Pages 110-115
[13] 杨颖,陈卫,田丰伟,等.产抑菌物质乳杆菌的筛选及性质的研究[J].工业微生物,2006,36(3)∶13-17
[14] 张任秀.乳杆菌DM9811脂肪酸组分抑制白色念珠菌致老年义齿性口炎的作用[J].中国老年学杂志,2011,24∶4787-4788
[15] Pangsomboon K, Kaewnopparat S, Pitakpornpreecha T, et al. Antibacterial activity of a bacteriocin from Lactobacillus paracasei HL32 against Porphyromonas gingivalis [J]. Arch Oral Biol, 2006, 51(9):784-93
[16] Nyako EA, Watson CJ, Preston AJ. Determination of the pH of peri - implant crevicular fluid in successful and failing dental implant sites:A pilot study [J]. Arch Oral Biol, 2005, 50(12)∶1055-1059
[17] 杨颖.口腔中益生乳杆菌的研究[D].江南大学,2006.DOI:10.7666/d.y1195887
[18] 杨娟,堵国成,陈坚,等.口腔乳酸杆菌的分离及其益生特性[J].微生物学报,2013,04∶403-408
[19] Kang MS,Na HS,Oh JS. Coaggregation ability of Weissella cibaria isolates with Fusobacterium nucleatum and their adhesiveness to epithelial Cells [J]. FEMS Microbiol Lett,2005,253(2)∶323-329