Expression and Clinical Significance of Nrf2 and HO-1 in Oral Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2017.02.022

Abstract

Abstract: Objective: To assess the potential significance of nuclear related factor-E2-factor and Heme oxygenase-1 in oral squamous cell carcinomas. Methods: Testing the expression of Nrf2 and HO-1 by immunohistochemistry,Super Vision,in 57 OSCC and 28 normal tissues,which without carcinoma cells,is to analysis the relationship between Nrf2 and HO-1 and to discuss the correlations between their expression and clinicopathological characteristics. Results: Result:Higher expression of Nrf2 and HO-1 was detected in OSCC than normal tissues(P＜0.05).Expression of Nrf2 and H0-1 was not related with age,gender,location,C-TNM,pT stage,smoking-index(P＞0.05).The expression of Nrf2 in OSCC was not related with differentiation and lymph node metastasis(P＞0.05).However,the expression of HO-1 was significantly correlated with differentiation and lymph node metastasis(P＜0.05).The expression of HO-1 was positively correlated with the expression of Nrf2 in OSCC(r=0.295,P=0.032). Conclusion: Present findings suggest that the expression of Nrf2 and HO-1 has significant role in the process of OSCC.

Key words: OSCC , Nrf2 , HO^-1

CLC Number:

R739.8

JIA Bao-chao,GUAN Jian, DAI Xin-peng, YANG Jian, WANG Jian-jie, LIU Dong-pu. Expression and Clinical Significance of Nrf2 and HO-1 in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2017, 33(2): 207-210.

References

[1] Chen Y, Azad MB, Gibson SB. Superoxide is the major reactive oxygen species regulating autophagy[J]. Cell Death and Differentiation, 2009, 16(17)∶1040-1052
[2] Pratheepa L, Ramani P, Sherlin HJ, et al. Expression of emerging novel tumor markers in oral squamous cell carcinoma and their clinical and pathological correlation to determine the prognosis and usefulness as a therapeutic target: a systematic review[J]. Journal of Natural Sciences Research, 2012, 2(1)∶57-66
[3] Paschos A, Pandya R, Duivenvoorden WC, et al. Oxidative stress in prostate cancer: changing research concepts towards a novel paradigm for prevention and therapeutics[J].Prostate Cancer and Prostatic Diseases, 2013, 16(3)∶217-225
[4] Suzuki T, Motohashi H, Yamamoto M.Toward clinical application of the Keap1-Nrf2 pathway[J]. Trends in Pharmacological Science, 2013, 34(6)∶340-346
[5] Bryan HK, Olayanju A, Goldring CE, et al. The Nrf2 cell defence pathway: Keap1-dependent and -independent mechanisms of regulation[J]. Biochem Pharmacol, 2013, 85(6)∶705-717
[6] Zhang M, An C, Gao Y, et al. Emerging roles of Nrf2 and phase II antioxidant enzymes in neuroprotection[J]. Progress in Neurobiology, 2013, 100(1)∶30-47
[7] Was H, Sokolowska M, Sierpniowska A, et al. Effects of heme oxygenase-1 on induction and development of chemically induced squamous cell carcinoma in mice[J]. Free Radical Biology and Medicine,2011,51(9)∶1717-1726
[8] Taguchi K, Motohashi H, Yamamoto M. Molecular mechanisms of the Keap1-Nrf2 pathway in stress response and cancer evolution[J].Genes Cells, 2011, 16(2): 123-140.
[9] 谢佐君.Nrf2、HO-1 在喉癌组织中的表达及其临床意义[J].广州医科大学学报,2013∶14-15
[10] DeNicola GM, Karreth FA, Humpton TJ, et al. Oncogene-induced Nrf2 transcription promotes ROS detoxification and tumorigenesis[J]. Nature,2011,475(7354)∶106-109
[11] Shibata T, Kokubu A, Gotoh M, et al. Genetic alteration of Keap1 confers constitutive Nrf2 activation and resistance to chemotherapy in gallbladder cancer. Gastroenterology,2008,135(4)∶1358-1368
[12] C.F. Huang,L .Zhang,S.R .Ma, et al. Clinical Significance of Keap1 and Nrf2 in Oral Squamous Cell Carcinoma[J].Plos One,2012,8(12)∶1-7
[13] P. sacca,R meiss, G. casas, et al. Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer[J].British Journal of Cancer,2007,97(12)∶1682-1689
[14] Torisu-Itakura H, Furue M, Kuwano M, et al. Co-expression of thymidine phosphorylase and heme oxygenase-1 in macrophages in human malignant vertical growth melanomas[J]. Cancer Science, 2000,91(9)∶906-910
[15] Tsuji MH, Yanagawa T, Iwasa S, et al. Heme oxygenase-1 expression in oral squamous cell carcinoma as involved in lymph node metastasis[J]. Cancer Letters,1999,138 (1-2)∶53-59
[16] Lee SS, Yang SF, Tsai CH, et al. Upregulation of heme oxygenase-1 expression in areca-quid-chewing-associated oral squamous cell carcinoma[J]. Journal of the Formosan Medical Association,2008,107(5)∶355-363
[17] Lavrovsky Y, Schwartzman ML, Levere RD, et al. Identification of binding sites for transcription factors NF-kappa B and AP-2 in the promoter region of the human heme oxygenase 1 gene[J]. Proceedings of the National Academy Sciences,1994,91(13)∶5987-5991
[18] McAllister SC, Hansen SG, Ruhl RA, et al. Kaposi sarcoma-associated herpesvirus (KSHV) induces hemeoxygenase-1 expression and activity in KSHV-infected endothelial cells.Blood[J]. 2004;103(9)∶3465-3467
[19] Mayerhofer M, Florian S, Krauth MT, et al. Identification of heme oxygenase-1 as a novel BCR/ABL-dependent survival factor in chronic myeloid leukemia[J]. Cancer Res. 2004;64(9)∶3148-3154