Role of Nrf2 in Liver Injury Induced by Periodontitis in Rats

doi:10.13701/j.cnki.kqyxyj.2020.12.008

Abstract

Abstract: Objective: To investigate the role of Nrf2 in liver injury induced by periodontitis in rats. Methods: 24 male Wistar rats were randomly divided into normal group and periodontitis group, with 12 rats in each group. The periodontitis model was established by 0.2mm orthodontic wire ligation and inoculation of Porphyromonas gingival on the neck of the first maxillary molar. After 6 weeks of modeling, periodontal clinical parameters were measured. Histopathological and immunohistochemical methods and qRT-PCR were used to detect the liver injury and the expression of Nrf2, Keap1, and NQO-1 mRNA and protein in liver tissues. Results: The periodontal tissues of rats in the periodontitis group showed obvious inflammation. The liver cord structure was disorder. Hepatocytes were degenerated and necrosis. mRNA expression levels of Nrf2 and NQO-1 were lower than that of the normal group, and Keap1 was higher than that of the normal group. The Nrf2 protein expression level in periodontitis group was lower than that in normal group. Conclusion: Nrf2 plays an important role in liver injury induced by periodontitis in rats.

Key words: periodontitis, liver injury, Nrf2

LI Yan, XIA Boyuan, LI Xin, DING Xu, WANG Wentian, YU Weixian. Role of Nrf2 in Liver Injury Induced by Periodontitis in Rats[J]. Journal of Oral Science Research, 2020, 36(12): 1113-1116.

References

[1] Romandini M, Laforì A, Romandini P, et al. Periodontitis and platelet count: A new potential link with cardiovascular and other systemic inflammatory diseases [J]. J Clin Periodontol, 2018, 45(11):1299-1310.
[2] Vasconcelos ACCG, Vasconcelos DFP, Pereira da Silva FR, et al. Periodontitis causes abnormalities in the liver of rats [J]. J Periodontol, 2019, 90(3):295-305.
[3] Chen M, Cai W, Zhao S, et al. Oxidative stress-related biomarkers in saliva and gingival crevicular fluid associated with chronic periodontitis: A systematic review and meta-analysis [J]. J Clin Periodontol, 2019, 46(6):608-622.
[4] Li X, Sun X, Zhang X, et al. Enhanced oxidative damage and Nrf2 downregulation contribute to the aggravation of periodontitis by diabetes mellitus [J]. Oxid Med Cell Longev, 2018, 2018:9421019.
[5] Ding Y, Ren J, Yu H, et al. Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice [J]. Immun Ageing, 2018, 15:6.
[6] Mester A, Ciobanu L, Taulescu M, et al. Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats [J]. J Periodontol, 2019, 90(8):911- 919.
[7] Tu W, Wang H, Li S, et al. The anti-inflammatory and anti-oxidant mechanisms of the Keap1/Nrf2/ARE signaling pathway in chronic diseases [J]. Aging Dis, 2019, 10(3):637-651.
[8] Du L, Wang L, Wang B, et al. A novel compound AB38b attenuates oxidative stress and ECM protein accumulation in kidneys of diabetic mice through modulation of Keap1/Nrf2 signaling [J]. Acta Pharmacol Sin, 2020, 41(3):358-372.
[9] Funakoshi-Tago M, Nonaka Y, Tago K, et al. Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 [J]. Sci Rep, 2020, 10(1):2584.
[10] Li Y, Zeng Y, Huang Q, et al. Helenalin from centipeda minima ameliorates acute hepatic injury by protecting mitochondria function, activating Nrf2 pathway and inhibiting NF-κB activation [J]. Biomed Pharmacother, 2019, 119:109435.
[11] 岳轶云,丁小函,李艳.Toll样受体4在慢性牙周炎诱发大鼠肝脏炎症中的作用[J].口腔医学研究,2019,36(2):139-142.

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