Effect of SMAS Modified PEGDA Hydrogel on Attachment and Proliferation of MC3T3-E1 Cells.

doi:10.13701/j.cnki.kqyxyj.2017.05.006

Abstract

Abstract: Objective: To investigate the effect of sodium methylallyl sulfonate (SMAS) modified poly(ethylene glycol) diacrylate (PEGDA) hydrogel on the attachment and proliferation of osteoblast-like cells (MC3T3-E1). Methods: A series of negative charged hydrogels were fabricated by incorporating charged monomer (SMAS) into PEGDA hydrogel at different concentrations. Scanning electron microscopy (SEM) was used to observe the surface morphology of the hydrogels. The protein adsorption of the different hydrogels was evaluated by BCA. To evaluate the cell attachment and proliferation on the hydrogel, the MC3T3-E1 cells were seeded onto the surface of hydrogels. Then, the cell morphology was observed at different time points. Furthermore, the cell proliferation was investigated by cell counting kit (CCK-8). Results: With increasing concentration of SMAS, the protein adsorption of the hydrogel was significantly enhanced (P<0.05). Meanwhile, compared to control group, the cell attachment of HG100 group and HG200 group were improved. The results of CCK8 assay showed that the cell proliferation was promoted on the modified hydrogels. Conclusion: The SMAS modified hydrogel can effectively promote the cell attachment and proliferation of MC3T3-E1 cells.

CLC Number:

R782

QU Fu-zhen, TAN Fei, LIU Jie.. Effect of SMAS Modified PEGDA Hydrogel on Attachment and Proliferation of MC3T3-E1 Cells.[J]. Journal of Oral Science Research, 2017, 33(5): 486-490.

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