Abstract: Objective: To analyze the expression profile variation of circle RNA (circRNA)during odontoblastic differentiation of mouse dental papilla cells and further investigate the functions of circRNA during the process. Methods: CircRNA microarray was performed to detect the difference of circRNA expression between primary mouse dental papilla cells with odontoblastic differentiation inducing or not. After pretreatment and homogenization of raw data, the circRNAs with different expression were screened out and then analyzed. Results: Compared with noninducing dental papilla cells, there were 4064 circRNAs differentially expressed which were more than 2 times variation and significant difference (P≤0.01) in odontoblastic differentiation inducing cells. Among them, 3255 were increased while 809 were reduced and the change′s fold was more than 2 times. Conclusion: The circRNA expression profile changed significantly during odontoblastic differentiation. The different circRNAs might be associated with the development of odontoblasts.

Key words: Odontoblastic differentiation, Circle RNA, Circle RNA microarray