Abstract: Objective: To fabricate fibrous barrier mat of poly(L-lactide-co-caprolactone)/silk fibroin loaded with PTH-Fc via electrospinning technique, and to study its biocompatibility, biodegradability, mechanical strength, and ability to regulate appropriate cellular activities. Methods: Electrospun fibrous poly(L-lactide-co-caprolactone)/silk fibroin loaded with PTH-Fc (PLCL/SF/PTH-Fc) or without (PLCL/SF) were prepared and compared. Apparent morphology before and after degradation were observed by scanning electron microscope (SEM). Tensile property was tested by custom mechanical testing device. The biocompatibility of PLCL/SF/PTH-Fc was evaluated by MTT and the cell adhesion assay. The osteogenic potential of the cells cultured with PLCL/SF/PTH-Fc was investigated by alizarin red stain. Results: The nanofibers of PLCL/SF/PTH-FC were uniform and smooth. The breaking strengths of PLCL/SF/PTH-Fc and PLCL/SF were (5.56±0.18) MPa and (5.42±0.03) MPa, respectively (P>0.05). PLCL/SF/PTH-Fc mats could degrade quickly, promote the cell proliferation, and induce the cell differentiation. Conclution: Electrospun fibrous PLCL/SF/PTH-Fc has good mechanical property and biodegradability. It can promote cell proliferation and cell differentiation.

Key words: Electrospinning, PTH-Fc, Guided bone regeneration, Fibrous barrier