Effects of Periodic Compression on Expression of Pro-osteoclastic Factors by Mandibular Condylar Chondrocytes

doi:10.13701/j.cnki.kqyxyj.2019.04.016

Abstract

Abstract: Objective: To investigate the effects of periodic compression on the expression of pro-osteoclastic factors by mandibular condylar chondrocytes (MCCs). Methods: The rat MCCs were isolated and mixed with agarose culture medium. The Flexcell system was used to conduct compression on the MCCs/agarose mixture. The intensities of compression were 15, 30, and 45 kPa (frequency 0.5 Hz), and the stimulating time were 2, 6, and 12 hours. The control group was treated in the same way but without compression. Cells were collected after the application of force and the expression of pro-osteoclastic factors by MCCs were detected. Results: When the MCCs were stimulated by 15 kPa compression for 6 hours, the expression of RANKL was significantly higher than those of the controls. When the MCCs were stimulated by 30 and 45 kPa compression for 6 and 12 hours, the expression of SDF-1, TGF-beta-1, and RANKL were higher than those of the controls, but the gene expression of MCP-1, M-CSF, and OPG were not different from those of the controls. Conclusion: Periodic compression could promote the expression of pro-osteoclastic factors such as SDF-1, TGF-beta 1, and RANKL.

Key words: Temporomandibular joint, Mandibular condylar chondrocytes, Compression, Osteoclast, Pro-osteoclastic factors

RUAN Guo-xian, GAO Li-qing, DENG Li. Effects of Periodic Compression on Expression of Pro-osteoclastic Factors by Mandibular Condylar Chondrocytes[J]. Journal of Oral Science Research, 2019, 35(4): 372-376.

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