Effect of Strontium Ranelate on Alveolar Bone Loss in Diabetic Periodontitis in Rats

doi:10.13701/j.cnki.kqyxyj.2021.06.019

Abstract

Abstract: Objective: To investigate the effects of strontium ranelate on alveolar bone loss in diabetic periodontitis in rats. Methods: Forty male SD rats weighing 200g were adopted and divided into four groups randomly: group D (diabetic rats with no intervention); group D+Sr (diabetic rats with administration of strontium ranelate intragastrically); group D+L (diabetic rats with ligature); and group D+L+Sr (diabetic rats with ligature and administration of strontium ranelate intragastrically). After 1 month of strontium ranelate administration, rats were sacrificed and serum and the jaw were collected to measure the content of Ca, P, and alkaline phosphatase (ALP) as well as the alveolar bone loss and expression of RANKL and OPG. Results: The contents of Ca and P among the four groups had no significant difference (P＞0.05), while content of ALP in D+L+Sr group was significantly higher than that in D+L group (P<0.05). The three dimensional reconstruction image of the alveolar bone showed that alveolar bone loss in D+L+Sr group was smaller than that in D+L group. Ratio of RANKL/OPG in D+L+Sr group was samller than that in D+L group. Conclusion: Strontium ranelate might promote bone formation and inhibit bone resorption via regulation of RANKL/OPG axis and eventually ameliorate alveolar bone loss in diabetic periodontitis.

Key words: strontium ranelate, diabetes, periodontitis, alveolar bone loss, RANKL/OPG

CHEN Helin, WANG Hang, HUANG Hui, WANG Yu, XIONG Yue. Effect of Strontium Ranelate on Alveolar Bone Loss in Diabetic Periodontitis in Rats[J]. Journal of Oral Science Research, 2021, 37(6): 569-573.

References

[1] Page RC, Offenbacher S, Schroeder HE, et al. Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions [J]. Periodontol 2000, 1997, 14: 216-248.
[2] Chen ZW, Yi HY, Hang HL, et al. Epidemiologic relationship between periodontitis and type 2 diabetes mellitus [J]. BMC Oral Health, 2020, 20(1): 204.
[3] Bonnelye E, Chabadel A, Saltel F, et al. Dual effect of strontium ranelate: stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro [J]. Bone, 2008, 42(1): 129-138.
[4] Yogui FC, Ervolino-Silva AC, Pitol-Palin L, et al. Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats [J]. Res Society Development, 2020, 9(10): e7539109092.
[5] Kirschneck C, Wolf M, Reicheneder C, et al. Strontium ranelate improved tooth anchorage and reduced root resorption in orthodontic treatment of rats [J]. Eur J Pharmacol, 2014, 744: 67-75.
[6] Karakan NC, Akpınar A, Göze F, et al. Investigating the effects of systemically administered strontium ranelate on alveolar bone loss histomorphometrically and histopathologically on experimental periodontitis in rats [J]. J Periodontol, 2017, 88(2): e24-e31.
[7] Marins LM, Napimoga MH, Malta FDS, et al. Effects of strontium ranelate on ligature-induced periodontitis in estrogen-deficient and estrogen-sufficient rats [J]. J Periodontal Res, 2019, 55(1): 141-151.
[8] Sun HY, Jiang H, Du MQ, et al. The prevalence and associated factors of periodontal disease among 35 to 44-year-old Chinese adults in the 4th national oral health survey [J]. Chin J Dent Res, 2018, 21(4): 241-247.
[9] Rizzoli R, Chapurlat RD, Laroche JM, et al. Effects of strontium ranelate and alendronate on bone microstructure in women with osteoporosis [J]. Osteoporos Int, 2012, 23(1): 305-315.
[10] Liu W, Zhang X. Receptor activator of nuclear factor-κB ligand (RANKL)/ RANK/ osteoprotegerin system in bone and other tissues [J]. Mol Med Rep, 2015, 11(5): 3212-3218.
[11] Wang S, Xu S, Shi Z, et al. Progress of research on the relationship between calcitonin gene-related peptide and RANK/RANKL/OPG system in the bone reconstruction [J]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 2019, 33(4): 511-515.
[12] Amin N, Boccardi V, Taghizadeh M, et al. Probiotics and bone disorders: the role of RANKL/RANK/OPG pathway [J]. Aging Clin Exp Res, 2020, 32(3): 363-371.
[13] Van Driel M, Van Leeuwen JP. Strontium ranelate promotes osteoblast differentiation and increases Opg/Rankl ratio in osteoblast-osteoclast co-cultures [J]. Bone, 2010, 47(1): s134.
[14] 王冰,徐又佳,郝彦明,等.去卵巢和激素致骨质疏松模型中骨密度与血清指标的比较[J].苏州大学学报(医学版),2008,28(1): 10-13.
[15] Nizet A, Cavalier E, Stenvinkel P, et al. Bone alkaline phosphatase: An important biomarker in chronic kidney disease-mineral and bone disorder [J]. Clin Chim Acta, 2020, 501: 198-206.