Antioxidant Effects of Nitro-oleic Acid on Radiation-damaged Rat Submandibular Gland Epithelial Cells via Nrf2/HO-1 Pathway

doi:10.13701/j.cnki.kqyxyj.2022.12.008

Abstract

Abstract: Objective: To detect whether nitro-oleic acid (OA-NO2) can exert antioxidant effect on radiation-damaged rat submandibular gland epithelial cells through Nrf2/HO-1 pathway. Methods: The submandibular gland epithelial cells of rats cultured in vitro for 3 days were established by electron linear accelerator, and the CCK-8 method was used to screen the best proliferation concentration of the radial injury cell model OA-NO2 after 24 h. According to the experimental purpose, they were divided into normal cell group (NG), radioactive cell group (RG), radioactive cell + nitro-oleic acid group (R+ONG), radiation cells + nitro-oleic acid + inhibitor group (R+ON+BRG), and radiation cells + inhibitor group (R+BRG). After 24 h of cell culture, the expressions of Nrf2, HO-1, and NQO1 protein were detected by Western blot, the production of reactive oxygen species was detected by reactive oxygen species reagent, and the proliferation rate was detected by CCK-8 method. Results: The CCK-8 results showed that: 4 μmol/L OA-NO2 effect for 24 h promoted the proliferation rate of radial injury submandibular gland epithelial cells best. After 24h of cell culture, in contrast to RG group, the expression of Nrf2, HO-1, and NQO1 proteins in R+ONG group increased significantly, and the production of reactive oxygen species decreased significantly (P<0.05). Compared with R+ONG, the expression of Nrf2, HO-1, and NQO1 protein in R+ON+BRG was significantly reduced and the production of reactive oxygen species was significantly increased (P<0.05). Conclusion: Nitro-oleic acid may exert antioxidant effect on rat submandibular gland epithelial cells after radiation injury through Nrf2/HO-1 pathway.

Key words: nitro-oleic acid, Nrf2/HO-1 signaling pathway, submandibular gland epithelial cells, radiation damage, antioxidant damage

LUO Qinliang, ZHANG Nini, LONG Yuanzhu, BAI Yu, DAI Min, TANG Jianhong, CUI Tianning, HUANG Guilin. Antioxidant Effects of Nitro-oleic Acid on Radiation-damaged Rat Submandibular Gland Epithelial Cells via Nrf2/HO-1 Pathway[J]. Journal of Oral Science Research, 2022, 38(12): 1139-1144.

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