Effect of Dicliptera Chinensis Polysaccharide on Expression and Significance of VEGF in Rat Parotid Gland after Radiation Injury.

doi:10.13701/j.cnki.kqyxyj.2019.02.006

Abstract

Abstract: Objective : To explore the regulation and significance of Dicliptera Chinensis Polysaccharide(DCP) on VEGF in rat parotid gland after radiation injury. Methods: Ninty-six adult SPF healthy male SD rats (200g-220g) were randomly divided into normal group (non-irradiated with 0.9% sodium chloride solution), radiation group (18Gy ⁶⁰Co γ-ray irradiation with 0.9% sodium chloride solution), normal treatment group (non-irradiated with 200 mg/kg Dicliptera Chinensis Polysaccharide solution), and radiotherapy group (18Gy ⁶⁰Co γ-ray irradiation with 200 mg/kg Dicliptera Chinensis Polysaccharide solution). Each group had 24 rats. All rats were weighed once a week to monitor the general condition. Hematoxylin-eosin staining (HE) was used to observe the morphological changes of the parotid gland in rats. The expression of VEGF was detected by real-time fluorescent quantitative polymerase chain reaction,FQ-PCR and Western blotting. Results: The weight loss of rats was observed in the radia tion group and the radiotherapy group. HE staining results showed that the salivary gland cells and blood vessels in the radiation group were damaged heavier. And the radiotherapy group had less damage. The relative expression of VEGF gene detected by FQ-PCR in radiation group was lower than other groups (P＜0.05). The expression of VEGF protein detected by WB in the radioactive group was lower than other groups (P＜0.05). Conclusion: DCP can alleviate parotid damage and up-regulate VEGF expression. DCP may repair the radial damage of rat parotid gland.

Key words: VEGF Radiation damage, Dicliptera chinensis polysaccharide, Parotid gland, Rat

QIN Shan-shan, WANG Dai-you,SUI Bin, ZHANG Yan,SUN Zhe.. Effect of Dicliptera Chinensis Polysaccharide on Expression and Significance of VEGF in Rat Parotid Gland after Radiation Injury.[J]. Journal of Oral Science Research, 2019, 35(2): 125-128.

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