Preparation and Osteogenic Properties of Melatonin-loaded Composite Barrier Membrane

doi:10.13701/j.cnki.kqyxyj.2023.03.011

Abstract

Abstract: Objective: To explore the physical and chemical properties of nano-barrier membranes loaded with different concentrations of melatonin (MT) and explore their effect on the proliferation and osteogenic differentiation of mouse embryonic osteoblast precursor cells (MC3T3-E1). Methods: SF/PLCL/MT biofilms loaded with different concentrations of MT (0, 0.01, 0.05, and 0.1 mol/L) were prepared by electrospinning technology. SEM, water contact angle, and FTIR were used to characterize the samples, and the degradation and sustained-release behaviors of barrier membranes were measured in an in vitro environment. The antioxidant capacity of the samples was determined by dpph assay. In addition, the biocompatibility of the samples was evaluated by CCK-8 and cell adhesion assay. ALP activity was detected to analyze the key enzymes of osteogenic differentiation, and RT-qPCR was used to test the mRNA expression levels of ALP, OCN, OPN, and Runx2. Results: MT was successfully loaded in SP and sustained for 27 days. The oxidation resistance of SP/MT was positively associated with the concentration of MT. In addition, the expression levels of key enzymes and mRNA related to osteogenic differentiation of SP/MT-0.1 mol/L were significantly higher than those of control group (P<0.05). Conclusion: SP/MT-0.1 mol/L barrier membrane has excellent physicochemical and biological properties and can promote the osteogenic differentiation potential of MC3T3-E1.

Key words: electrospinning, melatonin, barrier membrane, osteogenesis differentiation

LI Simei, LI Wanxin, YIN Lihua. Preparation and Osteogenic Properties of Melatonin-loaded Composite Barrier Membrane[J]. Journal of Oral Science Research, 2023, 39(3): 242-248.

References

[1] Benic GI, Hämmerle CH. Horizontal bone augmentation by means of guided bone regeneration [J]. Periodontol 2000, 2014, 66(1): 13-40.
[2] 朱艺丹,何苗苗,刘加涛,等.载PTH-Fc/PLCL/SF静电纺丝屏障膜的构建与性能研究[J].口腔医学研究,2018,34(8):913-918.
[3] Elgali I, Omar O, Dahlin C, et al. Guided bone regeneration: Materials and biological mechanisms revisited [J]. Eur J Oral Sci, 2017, 125(5): 315-337.
[4] Zhang KR, Gao HL, Pan XF, et al. Multifunctional bilayer nanocomposite guided bone regeneration membrane [J]. Matter, 2019, 1(3): 770-781.
[5] 王岩松,刘建林,陈晓霞.Poly(VBA-co-VBTAC)/XF-70的制备及抑菌作用的研究[J].口腔医学研究,2020,36(3):255-258.
[6] Qian Y, Chen H, Xu Y, et al. The preosteoblast response of electrospinning PLGA/PCL nanofibers: Effects of biomimetic architecture and collagen I [J]. Int J Nanomedicine, 2016, 11: 4157-4171.
[7] DeBari MK, King CI, Altgold TA, et al. Silk fibroin as a green material [J]. ACS Biomater Sci Eng, 2021, 7(8): 3530-3544.
[8] Zhang KH, Ye Q, Yan ZY. Influence of post-treatment with 75% (v/v) ethanol vapor on the properties of SF/P(LLA-CL) nanofibrous scaffolds [J]. Int J Mol Sci, 2012, 13(2): 2036-2047.
[9] Xie J, Li J, Ma J, et al. Magnesium oxide/poly(l-lactide-co-ε-caprolactone) scaffolds loaded with neural morphogens promote spinal cord repair through targeting the calcium influx and neuronal differentiation of neural stem cells [J]. Adv Healthc Mater, 2022, 11(15): e2200386.
[10] Wang X, Liu J, Jing H, et al. Biofabrication of poly(l-lactide-co-ε-caprolactone)/silk fibroin scaffold for the application as superb anti-calcification tissue engineered prosthetic valve [J]. Mater Sci Eng C Mater Biol Appl, 2021, 121: 111872.
[11] Yin L, Zhu Y, He M, et al. Preparation and characteristics of electrospinning PTH-Fc/PLCL/SF membranes for bioengineering applications [J]. J Biomed Mater Res A, 2020, 108(1): 157-165.
[12] Song W, Ma Z, Wang C, et al. Pro-chondrogenic and immunomodulatory melatonin-loaded electrospun membranes for tendon-to-bone healing [J]. J Mater Chem B, 2019, 7(42): 6564-6575.
[13] Zhu G, Ma B, Dong P, et al. Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathway [J]. Cell Biol Int, 2020, 44(2): 402-411.
[14] Zhang L, Zhang J, Ling Y, et al. Sustained release of melatonin from poly (lactic-co-glycolic acid) (PLGA) microspheres to induce osteogenesis of human mesenchymal stem cells in vitro [J]. J Pineal Res, 2013, 54(1): 24-32.
[15] Luo C, Yang Q, Liu Y, et al. The multiple protective roles and molecular mechanisms of melatonin and its precursor N-acetylserotonin in targeting brain injury and liver damage and in maintaining bone health [J]. Free Radic Biol Med, 2019, 130: 215-233.
[16] Xu X, Wang X, Qin C, et al. Silk fibroin/poly-(L-lactide-co-caprolactone) nanofiber scaffolds loaded with Huangbai Liniment to accelerate diabetic wound healing [J]. Colloids Surf B Biointerfaces, 2021, 199: 111557.
[17] Wang X, Liang T, Zhu Y, et al. Melatonin prevents bone destruction in mice with retinoic acid-induced osteoporosis [J]. Mol Med, 2019, 25(1): 43.
[18] Yang F, Yang L, Li Y, et al. Melatonin protects bone marrow mesenchymal stem cells against iron overload-induced aberrant differentiation and senescence [J]. J Pineal Res, 2017, 63(3).
[19] Zheng S, Zhou C, Yang H, et al. Melatonin accelerates osteoporotic bone defect repair by promoting osteogenesis-angiogenesis coupling [J]. Front Endocrinol (Lausanne), 2022, 13: 826660.
[20] MacDonald IJ, Tsai HC, Chang AC, et al. Melatonin inhibits osteoclastogenesis and osteolytic bone metastasis: Implications for osteoporosis [J]. Int J Mol Sci, 2021, 22(17):9435.
[21] Moradi SL, Golchin A, Hajishafieeha Z, et al. Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds [J]. J Cell Physiol, 2018, 233(10): 6509-6522.
[22] Mirmajidi T, Chogan F, Rezayan AH, et al. In vitro and in vivo evaluation of a nanofiber wound dressing loaded with melatonin [J]. Int J Pharm, 2021, 596: 120213.
[23] Cheng H, Yang X, Che X, et al. Biomedical application and controlled drug release of electrospun fibrous materials [J]. Mater Sci Eng C Mater Biol Appl, 2018, 90: 750-763.
[24] Galano A, Reiter RJ. Melatonin and its metabolites vs oxidative stress: From individual actions to collective protection [J]. J Pineal Res, 2018, 65(1): e12514.
[25] Reiter RJ, Tan DX, Rosales-Corral S, et al. Mitochondria: Central organelles for melatonin's antioxidant and anti-aging actions [J]. Molecules, 2018, 23(2): 509.
[26] Cao X. Targeting osteoclast-osteoblast communication [J]. Nat Med, 2011, 17(11): 1344-1346.
[27] Gulseren G, Yasa IC, Ustahuseyin O, et al. Alkaline phosphatase-mimicking peptide nanofibers for osteogenic differentiation [J]. Biomacromolecules, 2015, 16(7): 2198-2208.
[28] Barui A, Chowdhury F, Pandit A, et al. Rerouting mesenchymal stem cell trajectory towards epithelial lineage by engineering cellular niche [J]. Biomaterials, 2018, 156: 28-44.
[29] Wang X, Chen T, Deng Z, et al. Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p [J]. Stem Cell Res Ther, 2021, 12(1): 150.