Effect of Resin Infiltration Combined with Remineralization on Stability of Enamel White Spot Lesions in Oral Acid-base Environment

doi:10.13701/j.cnki.kqyxyj.2023.09.011

Abstract

Abstract: Objective: To observe and compare the changes of enamel roughness, hardness, and Ca/P ratio after resin infiltration combined with different remineralization treatment in oral acid-base circulation environment (pH cycle). Methods: A 4 mm×4 mm×2 mm enamel block was cut from 72 intact premolars in the center of the buccal surface of the crown. After acid-resistant nail polish was coated on its surface (except buccal side), it was placed in demineralization solution for 3 days to establish an in vitro demineralization model of enamel white spots. After resin infiltration treatment, half of the enamel surface was covered with acid-resistant nail polish as its own control side, and the other half was used as the experimental side. The samples were randomly divided into three groups (n=24). RI group was not treated with remineralization, RI/B group was treated with bioactive glass (twice a day), and RI/C group was treated with phosphopeptide containing fluorine casein/amorphous calcium phosphate (CPP-ACPF) (twice a day) for 7 days. Except for remineralization treatment, all samples were placed in pH cycle solution. The nail polish removed from the control side was recorded as before the pH cycle (time T₀) and after the pH cycle (time T₁) on the experimental side. The roughness, microhardness, and Ca/P ratio of each group of samples before and after pH cycle were observed and measured. Results: Both remineralization and pH cycle can significantly affect the enamel surface roughness and Ca/P ratio (P<0.05). After pH cycling, the roughness values of three groups of samples all increased, but the added value of RI/B group (T₁-T₀) was the least, followed by RI/C group and RI group increased the most (P<0.05). The microhardness of three groups decreased slightly without statistical difference (P>0.05). The Ca/P ratio of three groups decreased significantly (P<0.05), but the decrease in RI group was the most, and that in RI/B group was the least. Conclusion: After resin infiltration treatment of enamel white spot lesions, the roughness was increased, and the hardness and Ca/P ratio were decreased in pH cycle environment. Combined with bioactive glass or CPP-ACPF remineralization treatment, it can effectively improve the demineralization resistance of enamel and improve the stability of resin infiltration treatment

Key words: white spot lesions of enamel, resin infiltration, fluorinated casein phosphopeptide/amorphous calcium phosphate, bioactive glass, acid-base cycle

WANG Yan, NI Ke, HE Jingyu, LI Yingmei, MENG Qingfei. Effect of Resin Infiltration Combined with Remineralization on Stability of Enamel White Spot Lesions in Oral Acid-base Environment[J]. Journal of Oral Science Research, 2023, 39(9): 821-826.

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