Preparation of Capsaicin Nanoparticles and Their Effect on Treatment of Trigeminal Neuralgia in Rats

doi:10.13701/j.cnki.kqyxyj.2023.10.012

Abstract

Abstract: Objective: To prepare capsaicin (Cap) nanoparticles and develop them into gel to observe their therapeutic effect on trigeminal neuralgia in rats. Methods: Cap-loaded polylactic-co-glycolic acid/Cap nanoparticles (PLGA/Cap NPs) were prepared using PLGA as the carrier and bovine serum albumin as the stabilizer by emulsion solvent evaporation method. The nanoparticles were characterized by scanning electron microscopy-energy dispersive spectroscopy, Fourier transform infrared spectroscopy, high sensitivity zeta potential analyzer, and nanoparticle size analyzer. Differential scanning calorimetry was used to analyze the properties of the nanoparticles. The Cap nanoparticles were then prepared into gel with Carbopol 934. The drug loading capacity, encapsulation efficiency, and in vitro release performance were determined by high-performance liquid chromatography. Finally, they were applied to the surgical site of rat trigeminal neuralgia to observe the efficacy. Results: At a dose of 17 mg, PLGA/Cap NPs had regular spherical shape with hydrated particle size of (389.58±12.02) nm; polymer dispersity index (PDI) of 0.270±0.018; Zeta potential of (33.56±2.61) mV; drug loading capacity of (57.56±0.05)%; encapsulation efficiency of (90.26±0.90)%; and maximum cumulative release rate of 77.19%. After applying 0.03%, 0.3%, or 3% PLGA/Cap NP gels to the surgical site in rats' faces, trigeminal pain threshold was significantly increased (P<0.05). The level of substance P (SP) and prostaglandin E2 (PGE2) in serum were significantly decreased in the treatment group compared with the model group (P<0.05). Conclusion: Cap nano-gel has certain therapeutic effects on rat trigeminal neuralgia.

Key words: capsaicin, trigeminal neuralgia, substance P, prostaglandin E2

XIONG Yu, ZHENG Xinxin, CHEN Jinglin, CHEN Lizhen, JIN Youhong, WANG Pei. Preparation of Capsaicin Nanoparticles and Their Effect on Treatment of Trigeminal Neuralgia in Rats[J]. Journal of Oral Science Research, 2023, 39(10): 911-916.

References

[1] Sahin K, Kucuk O, Orhan C, et al. Bioavailability of a capsaicin lipid multi-particulate formulation in rats [J]. Eur J Drug Metab Pharmacokinet, 2021, 46(5): 645-650.
[2] Chen M, Xiao C, Jiang W, et al. Capsaicin inhibits proliferation and induces apoptosis in breast cancer by down-regulating FBI-1-mediated NF-κB pathway [J]. Drug Des Devel Ther, 2021, 15:125-140.
[3] Anantaworasakul P, Chaiyana W, Michniak-Kohn BB, et al. Enhanced transdermal delivery of concentrated capsaicin from chili extract-loaded lipid nanoparticles with reduced skin irritation [J]. Pharmaceutics, 2020, 12(5): 463.
[4] Puri N, Rathore A, Dharmdeep G, et al. A clinical study on comparative evaluation of the effectiveness of carbamazepine and combination of carbamazepine with baclofen or capsaicin in the management of trigeminal neuralgia [J]. Niger J Surg, 2018, 24(2): 95-99.
[5] 王明强,薛龙辉,熊鑫,等.PLGA改性聚氨酯微球的制备及性能研究[J].化工新型材料,2019,47(6):232-235.
[6] Haggag YA, Abosalha AK, Tambuwala MM, et al. Polymeric nanoencapsulation of zaleplon into PLGA nanoparticles for enhanced pharmacokinetics and pharmacological activity [J]. Biopharm Drug Dispos, 2021, 42(1): 12-23.
[7] Motawea A, Ahmed DAM, El-Mansy AA, et al. Crucial role of PLGA nanoparticles in mitigating the amiodarone-induced pulmonary toxicity [J]. Int J Nanomedicine, 2021, 16: 4713-4737.
[8] Mir M, Ahmed N, Rehman AU. Recent applications of PLGA based nanostructures in drug delivery [J]. Colloids Surf B Biointerfaces, 2017, 159: 217-231.
[9] 楼佳栋,张艳艳,马洁,等.Cu²⁺螯合封孔的介孔硅载药纳米颗粒的制备及应用[J].化工新型材料,2021,49(S1):136-140.
[10] He E, Li H, Li X, et al. Transdermal delivery of indirubin-loaded microemulsion gel: Preparation, characterization and anti-psoriatic activity [J]. Int J Mol Sci, 2022, 23(7): 3798.
[11] 厉志伟,王盛莉.高效液相色谱法测定辣椒中辣椒素、二氢辣椒素[J].中国调味品,2017,42(11): 123-126.
[12] 杨秀丽,羊波,李承乐.紫杉醇纳米脂质体凝胶剂的制备及体外透皮研究[J].中国现代应用药学,2015,32(2):156-161.
[13] László S, Bátai IZ, Berkó S, et al. Development of capsaicin-containing analgesic silicone-based transdermal patches [J]. Pharmaceuticals (Basel), 2022, 15(10): 1279.
[14] Lu Y, Zhang J, Lai W. Microglia activation and temporal changes in rat model of trigeminal neuralgia [J]. Hua Xi Kou Qiang Yi Xue Za Zhi, 2022, 40(6): 638-644.
[15] Li Y, Wang G, Guo Z, et al. Preparation of microcapsules coating and the study of their bionic anti-fouling performance [J]. Materials (Basel), 2020,13(7):1669.
[16] Kim D, Amatya R, Hwang S, et al. BSA-silver nanoparticles: A potential multimodal therapeutics for conventional and photothermal treatment of skin cancer [J]. Pharmaceutics, 2021, 13(4): 575.
[17] Jancso N. Pharmacological analysis of the function and receptor structure of the pain-sensitive nerve endings [J]. Acta Physiol Acad Sci Hung, 1957,11(Suppl):11-14.
[18] Malewicz NM, Rattray Z, Oeck S, et al. Topical capsaicin in poly(lactic-co-glycolic)acid (PLGA) nanoparticles decreases acute itch and heat pain [J]. Int J Mol Sci, 2022, 23(9): 5275.