Effect of Novel Oral Bacteriostatic Agents Based on Antimicrobial Peptides on Oral Bacterial Biofilms

doi:10.13701/j.cnki.kqyxyj.2023.12.005

Abstract

Abstract: Objective: To evaluate the effect of novel oral bacteriostatic agents based on antimicrobial peptides on oral bacterial biofilms by in vitro model. Methods: A 3-day-old oral bacterial biofilm model was constructed, and the effects of different contents on oral bacterial biofilm sterilization and inhibition of biofilm formation were analyzed by laser confocal microscopy and crystal violet staining experiment. Results: The antibacterial peptide buCaTHL4B had strong bactericidal ability against mature oral bacterial biofilms, and its sterilization rate was (42.4±2.7)%. When buCaTHL4B was used in combination with different concentrations of lysozyme solution and EDTA, the sterilization rates were [MIX 1:(49.2±1.8)%;MIX 2:(59.5±1.8)% and MIX 3:(59.2±1.7)%], respectively, which were better than those used alone (P<0.01). buCaTHL4B had a more durable bactericidal effect when combined with lysozyme at a concentration of 10 mg/mL. buCaTHL4B could inhibit biofilm formation more significantly when combined with lysozyme at a concentration of 10 mg/mL than buCaTHL4B alone. Conclusion: The mixed bacteriostatic solution based on small molecule polypeptide buCaTHL4B has good effect on the oral bacterial killing and formation inhibiting of biofilm.

Key words: antimicrobial peptides, lysozyme, EDTA, buCaTHL4B, oral bacterial biofilm

MIN Yifan, XIANG Yonggang, HU Ziqiu, MAO Min, XIA Lingyun. Effect of Novel Oral Bacteriostatic Agents Based on Antimicrobial Peptides on Oral Bacterial Biofilms[J]. Journal of Oral Science Research, 2023, 39(12): 1050-1055.

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