Effect of NaClO and EDTA Pretreatment on Resin Infiltration of Enamel White Spot Lesions: An in vitro Study

doi:10.13701/j.cnki.kqyxyj.2023.12.006

Abstract

Abstract: Objective: To compare the effect of different pretreatment agents on the pore exposure rate of the enamel surface, resin infiltration effectiveness, and surface microhardness of WSLs. Methods: A 4 mm×4 mm×2 mm enamel block from 96 sound premolars was cut in the center of the buccal surface of the teeth. The in vitro demineralization models of WSLs were established after placing the enamel blocks in the demineralization solution for 3 days. All samples were randomly divided into 4 groups (n=24): The samples in Group A given no pretreatment were control; Group B was pretreated with 5.25% NaClO solution; Group C was pretreated with 17% EDTA solution; Group D was pretreated with 5.25% NaClO and 17% EDTA solutions. All samples were etched with 15% hydrochloric acid after pretreatments. Eight samples of each group were used to calculate the exposure area and exposure rate of the pores in enamel surfaces. Another 8 samples were collected and used for evaluating the penetration depths and penetration area percentage after resin infiltration treatment of WSLs. The remaining 8 samples in each group were used to measure the surface microhardness of the enamel before and after resin infiltration. Results: Compared with group A, the exposed area and rate, resin penetration depth and percentage of penetration area, and surface microhardness of enamel in groups B, C, and D were significantly improved (P<0.05). After pretreated with 17% EDTA or 5.25% NaClO combined with 17% EDTA, the microhardness of demineralized enamel in groups C and D was significantly lower than that in groups A and B (P<0.05). Conclusion: Surface pretreatment with 5.25% NaClO or 17% EDTA can significantly increase enamel pore exposure rate, resin penetration depth, and surface microhardness, but pretreatment with 17%EDTA can reduce the microhardness of demineralized enamel.

Key words: white spot lesions, resin infiltration, pretreatment, NaClO, EDTA

NI Ke, WANG Yan, HE Jingyu, LI Yingmei, MA Yanhong, MENG Jian, MENG Qingfei. Effect of NaClO and EDTA Pretreatment on Resin Infiltration of Enamel White Spot Lesions: An in vitro Study[J]. Journal of Oral Science Research, 2023, 39(12): 1056-1062.

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