Preparation and Properties of Nano Low-crystalline Bio-Calcined Bone Repair Material

doi:10.13701/j.cnki.kqyxyj.2024.01.014

Abstract

Abstract: Objective: To study the effect of microstructure on osteogenesis property of nano low-crystalline bio-calcined bone repair material. Methods: The bio-calcined bone repair material was prepared by degreasing, deproteinizing and calcination, and its composition, structure, and biocompatibility were studied. The physical and chemical properties of the bio-calcined bone repair material were characterized, and then compared with those of natural calcined bovine bone. The influence of the differences on the osteogenic properties and safety were studied. Results: The main components of bio-calcined bone repair material and natural calcined bovine bone were low-crystalline hydroxyapatite, whose crystal sizes were all nanometer and whose pore size range and distribution were the same. The internal surface area of the bio-calcined bone repair material was 91.96 m²/g, and the bio-calcined bone repair material had acicular crystal. The internal surface area of natural calcined bovine bone was 52.51 m²/g, and natural calcined bovine bone had round crystal. The bio-calcined bone repair material retained more carbonate than natural calcined bovine bone. The biological test results showed that the bio-calcined bone repair material had good biocompatibility. The animal test results showed that both bio-calcined bone repair material and natural calcined bovine bone could effectively inhibit the alveolar bone atrophy and promote the repair of bone tissue after extraction, and there was no statistical difference between two materials. Conclusion: Bio-calcined bone repair material is a kind of nano low-crystalline bone repair material, which retains the composition and structure of inorganic salt of bone tissue, and has good biocompatibility. It is expected to be used in clinical practice.

Key words: calcined bone, low-crystalline, nanometer, hydroxyapatite, alveolar bone

ZHU Chao, ZHANG Tie, ZHANG Qi, HU Li, QIU Di, ZHANG Lipan, MA Xiaodan, WU Meng, LI Xiaoya, HUANG Aijun. Preparation and Properties of Nano Low-crystalline Bio-Calcined Bone Repair Material[J]. Journal of Oral Science Research, 2024, 40(1): 73-79.

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