Formononetin Motivates Osteogenic Differentiation of Dental Follicle Stem Cells

doi:10.13701/j.cnki.kqyxyj.2024.03.005

Journal of Oral Science Research ›› 2024, Vol. 40 ›› Issue (3): 214-220.DOI: 10.13701/j.cnki.kqyxyj.2024.03.005

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Formononetin Motivates Osteogenic Differentiation of Dental Follicle Stem Cells

TAO Tianyi¹, LI Zhengqiang², ZHENG Xiaoxue³, HANG Bing^1*

1. Department of Oral and Maxillofacial Surgery, Jilin University Stomatological Hospital, Key Laboratory of Tooth Development and Craniofacial Reconstruction and Regeneration, Changchun 130021, China;
2. Department of Oral and Maxillofacial Surgery, Stomatology Hospital, Southern Medical University, Guangzhou 510515, China;
3. Department of Otolaryngology, Oral-Maxillofacial Head and Neck Surgery, Jilin Province Cancer Hospital, Changchun 130012, China

Received:2023-11-06 Online:2024-03-28 Published:2024-03-25

Abstract

Abstract: Objective: To elucidate the effect of formononetin on osteogenic differentiation of human dental follicle stem cells (hDFSCs). Methods: hDFSCs were isolated, cultured, and identified. The cells were treated with different concentrations of formononetin (0, 0.1, 1, and 10 μmol/L) in the culture medium. Cell viability was assessed by CCK-8 assay, crystal violet staining, and live/dead cell fluorescence staining. The cell cytoskeleton was examined using fluorescent staining. The expression of osteogenic-related genes was analyzed through RT-qPCR. Alkaline phosphatase (ALP) activity was assessed to evaluate the osteogenic differentiation of cells. The influence of formononetin on calcium nodule formation during osteogenic differentiation was evaluated using alizarin red staining. Results: hDFSCs exhibited stem cell characteristics. Different concentrations of formononetin had no significant effect on hDFSCs viability. Whereas, 1 μmol/L formononetin facillitated the expression of Runx2, OCN, and Col-Iα1 mRNA. Additionally, 1 μmol/L formononetin enhanced the intracellular ALP activity and the number of calcium nodules. Conclusion: Formononetin at 1 μmol/L promotes the osteogenic differentiation of hDFSCs.

Key words: formononetin, human dental follicle stem cells, osteogenesis differentiation

TAO Tianyi, LI Zhengqiang, ZHENG Xiaoxue, HANG Bing. Formononetin Motivates Osteogenic Differentiation of Dental Follicle Stem Cells[J]. Journal of Oral Science Research, 2024, 40(3): 214-220.

References

[1] Meikai C, Xu YF, Zhang T, et al. Mesenchymal stem cell sheets: a new cell-based strategy for bone repair and regeneration [J]. Biotechnol Lett, 2019, 41(3):305-318.
[2] 徐静,吕慧欣,王一涵,等.Nell-1 蛋白通过Wnt/β-catenin通路调控人上颌窦黏膜来源间充质干细胞成骨分化[J].口腔医学研究,2013,39(3):227-235.
[3] Lucaciu O, Soriţǎu O, Gheban D, et al. Dental follicle stem cells in bone regeneration on titanium implants [J]. BMC Biotechnol, 2015, 15(1):114.
[4] 王健,孙瑜,陈磊,等.芒柄花素的现代研究进展[J].山西中医学院学报,2017,18(5): 74-76.
[5] 韩桂秋,王鸣刚,陈克明,等.芒柄花素对体外培养成骨细胞缺氧损伤的保护作用[J].中国药理学通报,2011,27(5): 671-677.
[6] 方瑶瑶,薛志远,杨秀艳,等.红芪中5种黄酮类成分对大鼠骨髓间充质干细胞和成骨细胞成骨分化的影响[J].中草药,2019,50(3): 632-638.
[7] 洪一波,姜宏,王建伟,等.芒柄花素抑制RANKL诱导破骨细胞分化的实验研究[J].中国骨伤,2020,33(1): 64-70.
[8] Gautam J, Khedgikar V, Kushwaha P, et al. Formononetin, an isoflavone, activates AMP-activated protein kinase/β-catenin signalling to inhibit adipogenesis and rescues C57BL/6 mice from high-fat diet-induced obesity and bone loss [J]. Br J Nutr, 2017, 117(5):645-661.
[9] Fulco M, Schiltzr L, Iezzi S, et al. Sir2 regulates skeletal muscle differentiation as a potential sensor of the redox state [J]. Mol Cell, 2003, 12(1): 51-62.
[10] Brown C, McKee C, Bakshi S, et al. Mesenchymal stem cells: Cell therapy and regeneration potential [J]. J Tissue Eng Regen Med, 2019, 13(9):1738-1755.
[11] 叶青松,王晓燕.牙源性干细胞储存和临床应用的研究进展[J].口腔疾病防治,2018,26(1): 15-25.
[12] Gautam AK, Bhargavan B, Tyagi AM, et al. Differential effects of formononetin and cladrin on osteoblast function, peak bone mass achievement and bioavailability in rats [J]. J Nutr Biochem, 2011, 22(4):318-327.
[13] Peng L, Wu F, Cao M, et al. Effects of different physical factors on osteogenic differentiation [J]. Biochimie, 2023, 207:62-74.
[14] Aubin J, Liu F, Malaval L, et al. Osteoblast and chondroblast differentiation [J]. Bone, 1995, 17(2): S77-S83.
[15] Komori T, Yagi H, Nomura S, et al. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts [J]. cell, 1997, 89(5): 755-764.
[16] Tsao YT, Huang YJ, Wu HH, et al. Osteocalcin mediates biomineralization during osteogenic maturation in human mesenchymal stromal cells [J]. Int J Mol Sci, 2017, 18(1):159-172.
[17] Tian Y, Xu Y, Fu Q, et al. Parathyroid hormone regulates osteoblast differentiation in a Wnt/β-catenin-dependent manner[J]. Mol Cell Biochem, 2011, 355(1-2):211-216.
[18] 樊梅,唐芳,马武开,等.Runx2基因参与Wnt/β-catenin信号通路中骨代谢疾病的研究进展[J].风湿病与关节炎,2019,8(10): 68-71.

Formononetin Motivates Osteogenic Differentiation of Dental Follicle Stem Cells

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