Study on Osteogenesis and Antibacterial Properties of 3D Printed Titanium Alloy Coated with Berberine Hydrochloride and Hydroxyapatite in Vitro

doi:10.13701/j.cnki.kqyxyj.2026.04.005

Abstract

Abstract: Objective: To improve the biocompatibility of Ti₆Al₄V by loading berberine hydrochloride (BBH) and hydroxyapatite (HA) on the surface after acid blasting. Methods: 3D printed Ti₆Al₄V samples were divided into SLA (control), HA (HA loaded), BBH (BBH loaded), and BBH/HA (BBH/HA loaded composite coating) groups after acid etching and sandblasting. BBH/HA composite coating was prepared on titanium alloy surface by electrochemical deposition method. The microstructure of the coating was observed by scanning electron microscope. The hydrophilic property and roughness of the coating were detected by contact angle measuring instrument and roughness measuring instrument. The bacteriostasis of each group to Staphylococcus aureus and Escherichia coli was analyzed by plate counting method. The effect on MC3T3-E1 cell proliferation was detected by cell proliferation test. The osteogenic ability of each sample was analyzed by alizarin red staining and quantitative pcr test. Results: The microscopic morphology observation results showed that the surface of each coating group was relatively uniform and firmly bonded to the substrate. The BBH/HA group was superior to other groups in terms of hydrophilicity, cell proliferation, and antibacterial activity. Conclusion: Berberine hydrochloride and hydroxyapatite loaded on the surface of Ti₆Al₄V after sandblasting can improve the osteogenic and antibacterial ability.

Key words: 3D printing, Ti₆Al₄V, berberine hydrochloride, hydroxyapatite, coating

WEI Zhaoming, SONG Nawei, YAN Xinghua, HAN Zekui, LIU Miao. Study on Osteogenesis and Antibacterial Properties of 3D Printed Titanium Alloy Coated with Berberine Hydrochloride and Hydroxyapatite in Vitro[J]. Journal of Oral Science Research, 2026, 42(4): 293-299.

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