Abstract: Objective: To determinate the performance of PEI derivatives of PEN loading ODN MT01 and discuss the proliferation of MG63 cells transfected with PEN/ODN MT01 complex. Methods: The following methods use Nanodrop to determinate the loading efficiency and Agarose gel electrophoresis to determinate the capability of PEN with ODN MT01 by different mass ration. We use the Malvin particle potential to test the particle size and potential of PEN/ODN MT01 complex. By FCM to ensure which is the best ration at the same time by the fluorescence observation of PEN loading ODN MT01-Cy5 which transfects MG63. We detemine the cells of proliferation rate of PEN/MT01 complex by MTT detection. Results: The surface of PEN/MT01 complex is positively charged. Particle size is 90-110 nm,low cytotoxicity,and the quality ration of ODN MT01 and PEN is 1∶6,the loading ration is 77.67%. What is more,the transfection of MG63 cell florescence intensity is the largest. Conclusion: The mass ration of PEN and ODN MT01 is 1∶6; the ODN MT01 can be efficiently loaded,and can obviously promote the proliferation of MG 63 cells.

Key words: ODN , MT01 , The derivative of , PEI , MG63 , Mass ration