Hematoporphyrinmonomethyl Ether Fluorescence Spectrometry Auxiliary Diagnosis of Early Oral Cancer

Abstract

Abstract: Objective: To investigate the feasibility of fluorescence spectra diagnosis of early oral cancer mediated with hematoporphyrinmonomethyl ether(HMME) in animal models. Methods: 21 golden hamsters were randomly divided into 3 groups (control group, 5 weeks group and 9 weeks group). Each group had 7 animals. After induced by DMBA in corresponding weeks all the cheek pouches of animal models in each group were injected through intraperitoneal by 20ug/ml HMME solution as 10ml/kg. HMME fluorescence and auto-fluorescence spectrums were detected by fluorescence spectroscopy diagnosis system and were analyzed by Origin7.0 to calculate fluorescence ratio K. All the detected points were determined by pathological diagnose. Mean K values were calculated and statistically analyzed by SPSS10.0. Results: The result of animal experiment was analyzed by ANOVA. It showed that the comparisons of K values among 3 experiment groups had statistical significance(P<0.01). Conclusion: Fluoresscent-spectro fluorometry method mediated by HMME could discriminate normal tissue, abnormal hyperplasia and cancer tissue. It had important reference value in diagnosis of early oral cancer.

Key words: Hematoporphyrinmonomethyl ether, Spectroscopy, Oral cancer, Diagnose, Golden hamsters

CLC Number:

R739.8

YANG Yan-qing, YUAN Jie, ZHANG Lei, et al. Hematoporphyrinmonomethyl Ether Fluorescence Spectrometry Auxiliary Diagnosis of Early Oral Cancer[J]. Journal of Oral Science Research, 2015, 31(7): 699-701.

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