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Analysis of Regional Accelerated Phenomenon in Corticotomy-assisted Orthodontic Treatment by Micro CT LIU Chang, LUAN Li-yang, JIANG Jiu-hui, et al 2015, 31(7): 641-644.  Abstract ( 209 )   PDF (1309KB) ( 218 )   Objective: To observe the rate of tooth movement and the structure of alveolar bone in corticotomy-assisted orthodontic treatment by Micro-CT. Methods: 75 four or six-week male SD rats were used in this study. The rats were randomly divided into 3 groups:group1-CO+TM, group2-TM, group3-CO, with 5 in each one group. The rats were sacrificed at different times(t=4,7,14,21,28days). Micro-CT was used to measure the change of tooth movement and the structure of alveolar bone. Results: In terms of the amount of tooth movement, our results illustrated that the CO+TM group had a small amount at the beginning of the study compared with the TM group, CO+TM group was obvious higher than TM group from day 14 to 28(P<0.05). From day 4 to 7, TM was faster than CO+TM, then slower than CO+TM. When compared to CO and TM, CO+TM continued to drop at day 4,7,21,28. TM group went up at first and then went down. CO group was stable at 0(P<0.05). The bone volume in mesial of CO+TM group always decreased. As to TM group, the volume decreased in the early, but rebound after the 4th day. Conclusion: Alveolar bone corticotomy-assisted orthodontic treatment can accelerat the rate of tooth movement from day 14 to 28. There is a phenomenon called "regional accelerated phenomenon(RAP)" occured at day 21, It is associated with increased perfusion and bone turnover and decreased bone density. References | Related Articles | Metrics

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Study on Dental Pulp Stem Cells(DPSCs) Regenerating Homogeneous Rats’ Pulp Dentin Complex TIE Xiao-min, LIU Jia, WANG Li-ping, et al 2015, 31(7): 645-648.  Abstract ( 199 )   PDF (877KB) ( 231 )   Objective: To observe the potential of homogeneous dental pulp stem cells (DPSCs) to repair and regenerate pulp dentin complex when rat crown pulp is removal. Methods: Eighteen SD rats were randomly divided into experimental group and control group. The experimental group was transplanted allogeneic rats’ DPSCs into the first molar’s pulp cavity whose crown pulp was destructed. The control group was not treated after crown pulp was destructed. After2, 4, 6weeks, 18 mice were sacrificed. Their upper jaw were investigated by general observation, X-ray, HE staining, and the thickness of dentin. Results: After 4 weeks, the hyperemia of experimental group reduced. After 6 weeks, X-ray could observe the dentin of experimental group was becoming thick and root apical foramen closed. After 6 weeks, newborn dentin in experimental group obviously increased in light microscope and odontoblasts arranged palisade. The sample in experimental group showed that after 2 weeks, dentin was becoming thick, and 4 weeks was more thick than it, continued to 6 weeks. The experimental group was better than the control group. Conclusion: It had potential clinical application value that the pulp cavity as the place of dental pulp stem cells implanting could promote pulp-dentin complex’s regeneration and repairing. References | Related Articles | Metrics

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Effect of bFGF Recombinant Lentiviral Vector on the Osteogenic Potential of Goat Bone Marrow-derived Mesenchymal Stem Cells HAN Xiang-zhen, WEI Wei, HE Hui-yu, et al 2015, 31(7): 649-652.  Abstract ( 154 )   PDF (890KB) ( 175 )   Objective: To study the effects of bFGF transfection on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Methods: Lentiviral vectors carrying bFGF were constructed to transfect sheep bone marrow mesenchymal stem cells. Cells were divided into two groups: bFGF group, and control group(non-transfected goat bone marrow mesenchymal stem cells). Real-time quantitative PCR and Western-blot were used to detect the changes of mRNA levels and protein levels of collagen-I, osteocalcin and osteopontin. Results: Expressions of collagen-I and osteopontin in mRNA levels were higher in transfection group than those in the control group, and the difference was statistically significant (P<0.05), But the expressions of osteocalcin in mRNA levels were not significant different in two group. Expression of OPN, Collagen- I, and OC of transfection group was higher than that in the control group, and the difference was statistically significant. Conclusion: bFGF transfection can enhance the ability of bone marrow mesenchymal stem cells on proliferation and osteogenic differentiation ,and they are suitable for seed cells for bone tissue engineering. References | Related Articles | Metrics

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Preliminary Study on Apoptosis Mechanism of Tongue Squamous Cell Carcinoma Cells Induced by Overexpressed -IL-18 GUO Yu, HOU Xu, LI Zheng-qiang, et al 2015, 31(7): 653-657.  Abstract ( 159 )   PDF (1476KB) ( 255 )   Objective:To investigate the effects of IL-18 overexpression on cell viability and apoptosis of tongue squamous cell carcinoma cells in vitro and in vivo. Methods: Human IL-18 cDNA was stably transfected into CRL1623 cells,which was confirmed by immunofluorescence, quantitative reverse transcription-PCR (q-PCR) and Western Blot. Flow cytometry, Giemsa staining and MTT assay were performed to detect changes of cell viability. q-PCR was performed to detect alteration of the expression of apoptosis-related genes. IL18-stably-transfected CRl1623 cells were incubated subcutaneously into BALB/c-nu mice to establish xenografted model to observe the effect of IL-18 overexpression on xenografted tumor growth in vivo. Results: The overexpression of IL-18 reduced TSCC cell viability via inducing cell apoptosis. Compared with CRL1623-Vec cells, IL-18 expression activated caspase 3, 7, and 9 via inducing their cleavage and increased the expression of interferon (IFN)-γ and cytochrome C mRNA expression in CRL1623-IL18 cells. Compared with control group(incubated with CRL1623-Vec),the tumor growth of experimental group(incubated with CRL1623-IL18) was obviously inhibited(P<0.05). Conclusion: The IL-18 protein can induce apoptosis of CRL1623 via initiating the intrinsic apoptosis pathway. References | Related Articles | Metrics

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Temporal-spatial Expression of Hyaluronic Acid in the First Mandibular Mouse Molar Tooth Germs YANG Guo-feng, MO Shen-zheng, JIANG Bei-zhan 2015, 31(7): 658-661.  Abstract ( 253 )   PDF (1005KB) ( 360 )   Objective: To investigate the expression patterns of hyaluronic acid (HA) in the first mandibular molar tooth germs, and to explore its role during the tooth development of mouse. Methods: Serial paraffin sections of the first mandibular embryonic molars at different stages were made. The expression patterns of HA were analyzed by immunohistochemistry. Results: The expression patterns of HA in the first lower molars varied among the different stages. It slightly expressed in the dental epithelium at tooth initiation stage. And it could be detected in the central areas of the tooth bud during the bud stage, while in the underlying dental mesenchyme the immunostaining was slight. From the cap stage to the late bell stage, the expression of HA at the stellate reticulum cells and dental papillae increased gradually. However, it never expressed in basement membrane, outer and inner enamel epithelium, and the areas where the ameloblast or the odontoblast located. Conclusion: The expression of HA was in a temporal-spatial specific pattern. Particularly, its expression in the stellate reticulum and dental papillae increased gradually with the development of tooth germ. This result implies that HA may be involved in tooth morphogenesis of the mouse molars. References | Related Articles | Metrics

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Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes WANG Ya-fei, WANG Si-han, GUO Xiao-kai, et al 2015, 31(7): 662-665.  Abstract ( 174 )   PDF (935KB) ( 322 )   Objective: To investigate the effect of different frequency of tensile stress on the proliferation and differentiation of mandibular condylar chondrocytes. Methods: Mandibular condylar chondrocytes (MCC) from two-week-old New Zealand rabbits were cultured in vitro under different frequency(0.1Hz,0.5Hz,1Hz) tensile stress for a period of 2 h per day for 3 days .The proliferation of articular chondrocyte was determined by CCK-8 assay. The gene expression (Sox9, Runx2, VEGF )was analyzed using real-time RT-PCR. Results: The static(0HZ) tensile stress had a significant stimulate on the chondrocyte proliferation compared with periodic tensile stress(0.1-1HZ)and the control group. The expression of Sox9, Runx2 and VEGF under 0.5HZ and 1HZ was significantly higher than the static(0HZ) tensile stress and the control group. Conclusion: There are different effects on the proliferation and differentiation of MCC under different frequency tensile stress. References | Related Articles | Metrics

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Adhesion Effects of Lemon Essential Oil on Main Pathogens of Periodontal Disease in Vtro BIAN Ke-yin, Zhang Xiang-yu, Jin Chen-xin, et al 2015, 31(7): 666-669.  Abstract ( 229 )   PDF (812KB) ( 387 )   Objective: To study the effect of lemon essential oil (LEO) on the adhesion of main pathogenic bacteria of periodontal disease, to explore its feasibility for the prevention and treatment of periodontal disease. Methods: Effect on the smooth polystyrene surface: The bacteria liquid and experimental groups of each component in the liquid mix were cultured in the 96 well plate for 48h. Bacteria adhesion was tested protein coated polystyrene adhesion surface: The bacteria solution and experimental group of each component in the liquid mix were cultured in the protein coated 96 well plates for 48h, Bacteria adhesion was tested. Results: Effect of smooth polystyrene surface adhesion: coneerning to Aa, Pi, there was no statistical significance between the LEO group and the control group; coneerning to Pg, Fn, there was significant difference between the LEO group and the control group (P<0.05); Protein coated polystyrene adhesion surface: coneerning to Aa, Pg, Pi, Fn, there was significant difference between LEO group and the control group (P<0.05). LEO could inhibit the adhesion of Aa, Pg, Pi, Fn on the protein coated surface, can inhibit the adhesion of Pg, Fn on the smooth surface. Conclusion: LEO can block the adhesion of pathogenic bacteria on the teeth surface, block the formation of the dendal plaque , and can prevent periodontal disease. References | Related Articles | Metrics

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Effect of Adding Ag-embedded Nano-TiO2 Antibacterial Agents on the Antibacterial Potential of Alginate Impression Materials GAO Shi-jun, WEI Si-yi, REN Guo-xin, et al 2015, 31(7): 670-672.  Abstract ( 160 )   PDF (819KB) ( 186 )   Objective: To study the effect of Ag-embedded nano-TiO2 (Ag - TiO2) antibacterial agent on the antibacterial of alginate impression materials, to analyze the appropriate ratio of the antibacterial agent to the alginate impression material. Methods: The Ag-embedded nano-TiO2 powder were added into the alginate impression materials according to different contents, including 0%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%,Their antibacterial activities were tested by film adhesion method. Results: Data showed the experimental group had statistically significant difference control group (all P<0.05). The difference increased with Ag-TiO2 antibacterial agent added mass ratio. The antibacterial efficiency was beyond 99% when the adding ratio of antibacterial agent was 2.0% or beyond 20%. Conclusion: Adding Ag-TiO2 antibacterial agent in alginate impression materials had antimicrobial potential, and the best adding ratio of the Ag-TiO2 antibacterial agent was 2.0%. References | Related Articles | Metrics

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Effect of Strontium Ranelate on Implant Osseointegration in Osteoporotic Rats WANG Mai-quan, WANG Yong-gong, PENG Li-wei, et al 2015, 31(7): 673-675.  Abstract ( 148 )   PDF (929KB) ( 263 )   Objective: To investigate the effect of strontium ranelate on osseointergration in osteoporotic rats. Method: Thirty-six female rats were randomly divided into three groups: ovariectomization group(A=12), strontium ranelate lower dose treatment group(B=12) and strontium ranelate high dose treatment group(C=12). Animals all received bilateral ovariectomy. After the success of the osteoporosis model building, HA coated titanium implants were inserted into proximal metaphyses of tibiae, meanwhile, the animals of group B and C were received oral medication strontium ranelate(B:450mg·kg-1·d-1 C:900mg·kg-1·d-1), Twelve weeks after surgery, animals were killed and undecalcified sections were prepared and examined histologically, micro-CT examnation and histomorphometrically. Results: 12 weeks after implantation surgery, BMD, IBCR and NBV in group B and C were significantly higher then those in group A(P<0.01). Conclusion: Strontium ranelate may resist the negative influence of osteoporosis and promote osseointagration of implant. References | Related Articles | Metrics

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MiR-124 Inhibits Proliferation of OSCC Cells by Targeting EZH2 HAN Zhe, MA Hui-qiang, PENG Cheng 2015, 31(7): 676-680.  Abstract ( 208 )   PDF (1008KB) ( 460 )   Objective: To assess the effect of miR-124 on proliferation of human OSCC cells and target relationship between miR-124 and EZH2. Methods: Pre-miR-124 sequence was synthesized and cloned into pcDNATM6.2-GW to construct recombinant plasmid pcDNATM6.2-GW-pre-miR-124 and identified by qRT-PCR. EZH2-WT and EZH2-MT sequences were respectively cloned into pmirGLO plasmid. The potential proliferation function of miR-124 on CAL27 cells was analyzed by MTT. The target relationship between miR-124 and EZH2 was identified by dual-luciferase assay system, qRT-PCR and western blot. Results: We constructed successfully the recombinant plasmids, including pcDNATM6. 2-GW-pre-miR-124, pmirGLO- EZH2-WT and pmirGLO- EZH2-MT, and the transfection efficiency of pre-miR-124 was increased in CAL27 cells compared to control group(P＜0.001). In addition, MTT assays showed that miR-124 could suppress the proliferation of CAL27 cells(P＜0.05). Furthermore, a significant reduction of luciferase activities of CAL27 cells cotransfected by pre-miR-124 and EZH2-WT(P＜0.001). The mRNA(P＜0.001) and protein expression levels of EZH2 were decreased in CAL27 cells transfected by pre-miR-124. Conclusion: Overexpression of miR-124 could suppress cell proliferation by targeting EZH2 in CAL27 cells. References | Related Articles | Metrics

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Detection of NLRP3 mRNA Expression in Periodontitis Patient's Own Tissue Nucleic Acid DING Zi-qing, SHEN Yu-qin, ZHOU Yue, et al 2015, 31(7): 681-683.  Abstract ( 267 )   PDF (913KB) ( 183 )   Objective: To determine the mRNA expression of NLRP3,NLRC4, and related genes IL-18,IL-1β,Caspase-1 mRNA in periodontitis patient's own tissue nucleic acid. To define a regulatory role of NLRP3 in periodontitis. Methods: Inflammatory periodontal tissue samples of chronic periodontitis patients were taken in periodontal flap surgery,and healthy gingival tissue samples were taken from orthodontic patients during tooth extractions.Total RNA from gingival tissue was extracted and reverse transcribed into cDNA, cryopreserved for further use. Real-time PCR was used to detect the mRNA expression of NLRP3,NLRC4, related genes IL-18,IL-1β, and Caspase-1 mRNA. Results: The mRNA levels of NLRP3,IL-18,IL-1β,Caspase-1 in the periodontitis patient's own tissue nucleic acid were higher than healthy gingival tissue. However, the mRNA expression of NLRC4 was not significant different between two groups. Conclusion: The high expression of NLRP3 and related genes IL-18, IL-1β, Caspase-1 mRNA in periodontitis patient's own tissue nucleic acid suggested that NLRP3 has an influence on the pathogenesis of periodontitis. References | Related Articles | Metrics

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Protein Kinase C Mediates Transforming Growth Factor β-induced integrin β6 Expression in Human Oral Squamous Cancer Cells GU Yin-zheng, DENG Xiao-ling, LI Qi-fu, et al 2015, 31(7): 684-687.  Abstract ( 214 )   PDF (1061KB) ( 138 )   Objective: To investigate whether protein kinase C (PKC) is involved in the expression of integrin β6 by human oral squamous cancer cells (SAS) after transforming growth factor β (TGF-β) stimulation. Methods: Human oral squamous cancer cells SAS were cultured with DMEM with 10% fetal bovine serum, after TGF-β stimulation, integrin β6 protein and mRNA levels in the cell lysate were measured by western-blot and quantitative real-time PCR, respectively. Effect of PKC inhibition on integrin β6 expression was investigated by using PKC inhibitors. Results: TGF-β induced SAS integrin β6 mRNA and protein expression levels in a time-dependent manner. These effects of TGF-β could be attenuated by the broad spectrum PKC inhibitor RO-31-8220 in a concentration-dependent manner, whereas Ca2+-dependent PKC inhibitor G6976 had no effect. Conclusion: Ca2+-independent PKC mediates TGF-β-induced integrin β6 expression in cultured SAS cells. References | Related Articles | Metrics

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STAT3 Promotes Human Lingual Squamous Cell Carcinoma Invasion and Metastasis ZHAO Yan, ZHANG Bi, CHEN Qi, et al 2015, 31(7): 688-690.  Abstract ( 221 )   PDF (916KB) ( 225 )   Objective: To investigate the expression of signal transducers and activators of transcription 3 (Stat3) in human lingual squamous cell carcinoma and to evaluate the role of STAT3 for tumor cell invasion and metastasis. Methods: The immunohistochemistry method was used to detect the expression of STAT3 in human lingual squamous cell carcinoma. The human lingual squamous cell carcinoma cell line, Tca-8113 cells were transfected with STAT3 small interfering RNA(siRNA). Transwell assay was used to study cell invasion. Results: There was a significant correlation between STAT3 expression and stages (P=0.029) and lymph node metastasis (P=0.009). The specific knockdown of STAT3 by RNA interference strongly inhibited the motile and invasion activity of tumor cells. Conclusion: STAT3 promotes human lingual squamous cell carcinoma invasion and metastasis. STAT3 siRNA can inhibit the invasion ability of tumor cells. References | Related Articles | Metrics

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Effects of Three Desensitizers on Bonding Strength of the Zirconia Ceramic GUAN Zhen-jie, CHEN Lei, DONG Xin, et al 2015, 31(7): 691-694.  Abstract ( 163 )   PDF (1031KB) ( 220 )   Objective: To evaluate the effects of three desensitizers on bond strength of zirconia ceramic. Methods: Freshly extracted human premolars were exposed dentinal tubules. Three desensitizers(Hybrid Coat, Adper Easy One, Duraphat suspension) were used in corresponding groups by different time. Zirconia ceramic slices were bonded to dentine surfaces with resin-modified glass ionomer cements (RMBIC) and resin cements (RC) respectively. The prepared teeth without desensitizer were used as control group. Specimens were tested by a universal testing machine to obtain the shear bond strength of each specimen and measured micro leakage by magenta-staining method . The value was analyzed by SPSS 19.0 statistical software. Results: The difference of shear strength value between Hybrid Coat group and the control group had no statistical significance; The shear strength value of Adper Easy One group was higher than the control group (P<0.05); The shear strength value of Duraphat group was lower than the control group (P<0.05); The shear strength value of after preparatory group was higher than prior bonding group (P<0.05). RMGIC showed greater strength than RC (P<0.05). Conclusion: Hybrid Coat and Adper Easy One could be chosen for desensitization when bonding zirconia ceramic restoration desensitize should be used after vital pulp abutment teeth preparation to reduce the postoperative sensitivity and enhance the bond strength of dentin interface. The bond strength of RC is higher than RMGIC. References | Related Articles | Metrics

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Chin and Lip Esthetic Changes Analysis in Class Ⅱ Division 2 Malocclusion of Adults Treated by Non-extraction Protocol WEI Jia-dai, LIU Jian-ying, MO Shui-xue 2015, 31(7): 695-698.  Abstract ( 189 )   PDF (957KB) ( 242 )   Objective: To investigate the soft and hard tissue esthetic changes of the chin and lip in Class Ⅱ division 2 malocclusion of adults treated by non-extraction. Methods: Twenty adult patients were enrolled and orthodontically treated without extraction. The radiographs were processed using CASSOS Image software. The data was analysed statistically to compare the soft and hard tissue esthetic changes of the chin and lip before and after the treatment. Results: After the treatment, the angles of U1-SN,L1-MP, and the distance of U1-AP,U1-NA, L1-AP,L1-NB were greater than those in pre-treatment. Meanwhile, U1-L1 decreased. Crowns of incisors were compensatorily moved close to labial side so as to acquire proper overbite and overjet. The distance of LLP,EP-LL increased obviously, and the lower lip was moved labially. Conclusion: Patients can not only relieve atresia relationship of anterior teeth but also improve the esthetic curve of chin and lip by protrusion of lower lip after non-extraction treatment. References | Related Articles | Metrics

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Hematoporphyrinmonomethyl Ether Fluorescence Spectrometry Auxiliary Diagnosis of Early Oral Cancer YANG Yan-qing, YUAN Jie, ZHANG Lei, et al 2015, 31(7): 699-701.  Abstract ( 230 )   PDF (880KB) ( 265 )   Objective: To investigate the feasibility of fluorescence spectra diagnosis of early oral cancer mediated with hematoporphyrinmonomethyl ether(HMME) in animal models. Methods: 21 golden hamsters were randomly divided into 3 groups (control group, 5 weeks group and 9 weeks group). Each group had 7 animals. After induced by DMBA in corresponding weeks all the cheek pouches of animal models in each group were injected through intraperitoneal by 20ug/ml HMME solution as 10ml/kg. HMME fluorescence and auto-fluorescence spectrums were detected by fluorescence spectroscopy diagnosis system and were analyzed by Origin7.0 to calculate fluorescence ratio K. All the detected points were determined by pathological diagnose. Mean K values were calculated and statistically analyzed by SPSS10.0. Results: The result of animal experiment was analyzed by ANOVA. It showed that the comparisons of K values among 3 experiment groups had statistical significance(P<0.01). Conclusion: Fluoresscent-spectro fluorometry method mediated by HMME could discriminate normal tissue, abnormal hyperplasia and cancer tissue. It had important reference value in diagnosis of early oral cancer. References | Related Articles | Metrics

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Decompression Combined with Reformed Space Maintainer: A Conservative Approach for Treating Dentigerous Cyst in Children with Mixed Dentition YU Yao-Hui, CHEN Wei, MEI Li-Qin, et al 2015, 31(7): 702-704.  Abstract ( 153 )   PDF (838KB) ( 245 )   Objective: To evaluate the efficacy of the decompression associated with reformed space maintainer for the large dentigerous cysts in children. Methods: 11 patients with large dentigerous cysts were underwent decompression operations. Cystic fluid was evacuated continuously. The cyst cavity was kept open and packed with iodoform gauze. The patients wear the reformed space maintainer after 1 week. Patients were followed-up for 6-24 months. Results: 11 cases of cysts were disappear and the bone defect ossified. 1 of 11 patients needed additional orthodontic treatment. Embedded permanent teeth of all patients erupted into dental arch or evidently moved upward. No patients were reported the sign of persistence or the recurrence of cyst. Conclusion: The decompression associated with reformed space maintainer is a safe, reliable, effective method in treating large dentigerous cyst in children. This not only can satisfactory restore esthetics and function, but also prevents the children from psychosocial trauma. References | Related Articles | Metrics

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Effect of pH on the Fatigue Strength of Vitallium and Co-cr Clasps ZHANG Han-fang, XIE Wei-li, GAO Hua-dong, et al 2015, 31(7): 705-707.  Abstract ( 151 )   PDF (909KB) ( 185 )   Objective: To compare fatigue attenuation of high Co-Cr-Mo alloy and Co-Cr alloy clasps which were in the same deformation and cyclic number under different pH values. Methods: V and W were used to cast twenty straight clasps respectively which were divided into 4 groups and tested in the air and in the artificial saliva of pH equal to 4.0,6.7 and 8.0 separately.Then the load-deformation test was carried on specimens to get terminal load as a percentage of initial load with the universal testing machine for 0.8 mm deformation and condition of cyclic loading 20000 times. Surfaces were examined by SEM. Results: The attenuation of W group was higher than that of V group. The minimum ones was in the air. And specimen attenuation increased with increment of experimental pH value. The attenuation of V was the most in the pH=4 group. Conclusion: Using Vitallium 2000 plus casting clasps, the removable partial denture can keep the longer initial treatment effect. References | Related Articles | Metrics

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Efficacy of Haemangioma Treated by Propranolol and the Treatment’s Complications in Xinjiang Region LING Bin, YING Xiao-peng, ZHANG Jing, et al 2015, 31(7): 708-711.  Abstract ( 153 )   PDF (918KB) ( 252 )   Objective:To evaluate the efficacy and respond to adverse reaction of propranolol in the treatment of infantile hemangiomas. Methods:A total of 50 infantile hemangiomas patients aged 1-18m were treated with propranolol at the dosage of 2mg/kg/d. The changes of tumor size, texture and color were monitored and recorded at a regular interval. The adverse effects after medication were observed. The short-term results were evaluated using a Achauer point scale system and color doppler ultrasound B. The adverse effects after medication therapy were observed and managed accordingly. Results:All 50 patients were followed up for 12months, the response was excellent in 11 patients, good in 18, moderate in 17, and poor in 4. There was no statistically significant between sex, race, tumor location and treatment efficacy. There was significantly different between the various type of hemangiomas(P<0.05).There were 16 patients who present various complications after treatment. Conclusion: Propranolol can be safely and effectively used to treat infantile hemangiomas. And no major side-effects were observed. This treatment should be considered as a promising alternative. References | Related Articles | Metrics

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Effects of Dissection with Anterograde and Retrograde Methods on the Function of Facial Nerve in Parotidectomy OU De-ming, WU Yin, LIU Jun, et al 2015, 31(7): 712-714.  Abstract ( 157 )   PDF (782KB) ( 282 )   Objective: To compare the effects of dissection methods on the fanction of facial nerve in parotidectomy. Methods: 107 cases were randomly treated with anterograde (48 cases)and retrograde (59 cases)dissection methods. Operation time was calculated , and the ratio of facial paralysis and functional recovery period were observed. Results: Permanent facial paralysis didn’t happened in all cases. Operation time, the ratios of temporary facial paralysis and functional recovery period of the patients in anterograde group and retrograde group were 46.33±4.85min,14.58%,2.04±0.95 month and 42.51±4.23 min,33.90%,2.47±0.97 month. Operation time showed no significant difference, and the ratios of temporary facial paralysis and functional recovery period of the patients showed significant difference(P<0.05). Conclusion: Using anterograde dissection method adroitly in parotidectomy may be a better choice to reduce the functional damage of facial nerve. References | Related Articles | Metrics

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Reliability of CBCT-based 3D Landmarks Identification SHI Jian-lu, ZHENG Lu-yan, YANG Zhi-hui, et al 2015, 31(7): 715-718.  Abstract ( 319 )   PDF (855KB) ( 279 )   Objective: To evaluate the reliability of identification of CBCT-based skull landmarks and to provide reference for the three-dimensional cephalometric analysis in the future. Methods: 30 pretreatment CBCT data were randomly selected from the CBCT database. Dolphin 11.5 was used to identify 14 skull landmarks in the sagittal, coronal,and axial slices on 3D images by five observers. A principal observer repeated five times and the other four observers three times within a 1-week interval. Intra-class correlation coefficient (ICC) for the values of the coordinate x, y, z of each landmark was calculated to determine Intra-observer and inter-observer reliability. Results: The ICC was ＞0.90 for 64.3%,＞0.75 for 90.5% ,and≤0.45 for only 2.4% of inter-observer assessments. The ICC of the intra-observer assessments was ＞0.90 for 88.1%,＞0.75 for 95.2% ,and none of intra-observer ICC was ≤0.45. Conclusion: Using Dolphin software to identify landmarks on CBCT images can offer excellent reliability with appropriate landmark definition and well-trained observers. References | Related Articles | Metrics

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Study on the Diversity of Subgingival Microflora in Implant Mucositis CHEN Xin, XIONG Ping, ZHONG Ke, et al 2015, 31(7): 719-723.  Abstract ( 273 )   PDF (1055KB) ( 364 )   Objective: To explore the diversity of subgingival microflora in implant mucositis and help to choose proper maintenance and intervention method. Methods: Microbial total DNA of gingival crevicular fluid samples from 10 cases which had been diagnosed as implant mucositis and 14 healthy implants. PCR (polymerase chain reaction) was conducted with bacteria 16S rDNA-V3 region as primer. The amplification products were analyzed using DGGE (denaturing gradient gel electrophoresis) and the main DNA bands of DGGE were sliced and used for sequence analysis. Results: Significant statistical differences were observed between group A and group B on PICF index (P<0.01). UPGMA cluster analysis of two groups showed 18% similarity overall. Group A:The similarity ranged from 29.9% to 60.2%; group B: the similarity ranged from 30.8% to 74%. Five phylums were detected in group A, including fifteen genus, which contained an uncultured bacterium, and Candidate division TM7 phylum was found only in this group; Four phylums were detected in group B, including twelve genus. Conclusion: Gingival crevicular fluid volume increased in peri-implant mucositis when compared with healthy implants. Microbial communities of samples in both healthy implants and implants with mucositis showed high diversities, while showed big difference in microbial community structures. The proportion of gram negative anaerobic bacillus increased as peri-implant mucositis occurred. References | Related Articles | Metrics

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In Situ Regeneration of Enamel-like Fluoridated Hydroxyapatite on Enamel Surface under Near Physiological Conditions XI Hong, ZHANG Kai, DONG Zhi-heng, et al 2015, 31(7): 724-727.  Abstract ( 138 )   PDF (1165KB) ( 237 )   Objective: To achieve in situ regeneration of enamel-like fluoridated hydroxyapatite on enamel surface under near physiological conditions. Methods: FA enamel-like structure was produced on the enamel surface of human teeth by a simple chemical method using HEDTA,CaCl2,NaH2PO4·2H2O,and NaF as the reactants under the condition of 37℃ water bath for a period of time. The physicochemical and biological properties of the enamel-like structure were studied by XRD,FTIR,SEM,XPS,MTT assay and bacterial inhibition ring test. Results: The compacted well-aligned FA crystals were produced,which could grow directly on the enamel surface. Conclusion: The synthetic structure under the condition of near physiological conditions can preliminarily mimic the shape and structure of enamel, and has the biocompatibility and certain antibacterial function. References | Related Articles | Metrics

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Prediction about Mesiodistal Crown Diameters of Teeth from Different Classes of Malocclusion Based on Genetic Algorithms Method DONG Yan, YANG Lu- yi, ZHU Hui-fang, et al 2015, 31(7): 728-731.  Abstract ( 181 )   PDF (805KB) ( 190 )   Objective: To establish an equation between mesiodistal crown diameters of different types of malocclusion teeth and other teeth, and to use just one equation to describe the situation of the teeth with same position and same gender. Methods: Pre-treatment casts of malocclusion patients' diagnosed as classⅠ, Ⅱ, Ⅲ were screened out. 100 for each type, and 50 males 50 females respectively. Among these, 45 were designed as analog samples and the other 5 casts were set as test samples. An equation for these three classes of malocclusion was established between the mesiodistal teeth diameter measured by a vernier caliper and that of the other teeth, and then compared with linear regression analysis. Results: The equation between mesiodistal crown diameters of different types of malocclusion teeth and that of other teeth was established. Comparing the predictive value with measured value, there existed no statistical significance neither GAS nor linear regression analysis but the absolute mean error of test samples by GAS was less than by linear regression analysis. Conclusion: The prediction accuracy by GAS is better than by linear regression analysis method.The established equation is of value to do full dentition gap analysis for different types of malocclusion. References | Related Articles | Metrics

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Clinical Observation of Orthodontic Treatment and Orthodontic-Prosthodontic Combined Treatment for Congenitally Missing Teeth Patients ZHOU Lei, WANG Chuan-jiang, WEI Yu-hua 2015, 31(7): 732-734.  Abstract ( 171 )   PDF (850KB) ( 458 )   Objective: To explore the clinical effect of congenitally missing teeth patients who were given orthodontic or orthodontic-prosthodontic combined treatment. Methods: The congenitally missing teeth patients were examined and given detailed treatment plan. They were treated with extraction orthodontic treatment or orthodontic--prosthodontic combined treatment. Results: The congenitally missing teeth patients who were treated with different methods achieved good facial profiles and normal overjet and overbite. Conclusion: The congenitally missing teeth patients will achieve satisfactory effects if they are given correct treatment plan according to facial profile, position and number of missing teeth, crowding, overjet, molar relation, and etc. References | Related Articles | Metrics

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Effect of Modified Platelet-rich Plasma on the Mineralization of Human Bone Marrow Stromal Cells LI Shi-han, DUAN Jian-min, WEN Jun, et al 2015, 31(7): 735-738.  Abstract ( 220 )   PDF (1008KB) ( 253 )   Objevtive: To investigate the effect of platelet-rich plasma (PRP) at different concentrations on the mineralization of human bone marrow stromal cells. Methods: BMSCs from healthy donor were cultured and passaged for 3-4 passages. PRP were manufactured from venous blood of the same donor. The activity of alkaline phosphatase in BMSCs was detemined using alkaline phosphatase reagent kit on the 7th day after mineralization induction. The formation of mineralized nodules in BMSCs were observed by alizarin red staining on the 21th days after minralization induction. After 7 days of minralization induction the expression of gene Runx2 in BMSCs was measured by real-time PCR. Results: PRP at the concentration of 5% evidently promoted the alkain phosphatase activity and the formation of mineralized nodules in BMSCs. The expression of gene Runx-2 was also evidently enhanced. Conclusion: PRP at the concentration of 5% could promote the mineralization of BMSCs. References | Related Articles | Metrics