Preliminary Study on Apoptosis Mechanism of Tongue Squamous Cell Carcinoma Cells Induced by Overexpressed -IL-18

Abstract

Abstract: Objective:To investigate the effects of IL-18 overexpression on cell viability and apoptosis of tongue squamous cell carcinoma cells in vitro and in vivo. Methods: Human IL-18 cDNA was stably transfected into CRL1623 cells,which was confirmed by immunofluorescence, quantitative reverse transcription-PCR (q-PCR) and Western Blot. Flow cytometry, Giemsa staining and MTT assay were performed to detect changes of cell viability. q-PCR was performed to detect alteration of the expression of apoptosis-related genes. IL18-stably-transfected CRl1623 cells were incubated subcutaneously into BALB/c-nu mice to establish xenografted model to observe the effect of IL-18 overexpression on xenografted tumor growth in vivo. Results: The overexpression of IL-18 reduced TSCC cell viability via inducing cell apoptosis. Compared with CRL1623-Vec cells, IL-18 expression activated caspase 3, 7, and 9 via inducing their cleavage and increased the expression of interferon (IFN)-γ and cytochrome C mRNA expression in CRL1623-IL18 cells. Compared with control group(incubated with CRL1623-Vec),the tumor growth of experimental group(incubated with CRL1623-IL18) was obviously inhibited(P<0.05). Conclusion: The IL-18 protein can induce apoptosis of CRL1623 via initiating the intrinsic apoptosis pathway.

Key words: IL-18, Tongue squamous cell carcinoma, Gene expression, Cell apoptosis, Xenografted model

CLC Number:

R739.8

GUO Yu, HOU Xu, LI Zheng-qiang, et al. Preliminary Study on Apoptosis Mechanism of Tongue Squamous Cell Carcinoma Cells Induced by Overexpressed -IL-18[J]. Journal of Oral Science Research, 2015, 31(7): 653-657.

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