MiR-124 Inhibits Proliferation of OSCC Cells by Targeting EZH2

Abstract

Abstract: Objective: To assess the effect of miR-124 on proliferation of human OSCC cells and target relationship between miR-124 and EZH2. Methods: Pre-miR-124 sequence was synthesized and cloned into pcDNATM6.2-GW to construct recombinant plasmid pcDNATM6.2-GW-pre-miR-124 and identified by qRT-PCR. EZH2-WT and EZH2-MT sequences were respectively cloned into pmirGLO plasmid. The potential proliferation function of miR-124 on CAL27 cells was analyzed by MTT. The target relationship between miR-124 and EZH2 was identified by dual-luciferase assay system, qRT-PCR and western blot. Results: We constructed successfully the recombinant plasmids, including pcDNATM6. 2-GW-pre-miR-124, pmirGLO- EZH2-WT and pmirGLO- EZH2-MT, and the transfection efficiency of pre-miR-124 was increased in CAL27 cells compared to control group(P＜0.001). In addition, MTT assays showed that miR-124 could suppress the proliferation of CAL27 cells(P＜0.05). Furthermore, a significant reduction of luciferase activities of CAL27 cells cotransfected by pre-miR-124 and EZH2-WT(P＜0.001). The mRNA(P＜0.001) and protein expression levels of EZH2 were decreased in CAL27 cells transfected by pre-miR-124. Conclusion: Overexpression of miR-124 could suppress cell proliferation by targeting EZH2 in CAL27 cells.

Key words: OSCC, miR-124, EZH2, Cell proliferation

CLC Number:

R739.8

HAN Zhe, MA Hui-qiang, PENG Cheng. MiR-124 Inhibits Proliferation of OSCC Cells by Targeting EZH2[J]. Journal of Oral Science Research, 2015, 31(7): 676-680.

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