Abstract: Objective: To observe the potential of homogeneous dental pulp stem cells (DPSCs) to repair and regenerate pulp dentin complex when rat crown pulp is removal. Methods: Eighteen SD rats were randomly divided into experimental group and control group. The experimental group was transplanted allogeneic rats’ DPSCs into the first molar’s pulp cavity whose crown pulp was destructed. The control group was not treated after crown pulp was destructed. After2, 4, 6weeks, 18 mice were sacrificed. Their upper jaw were investigated by general observation, X-ray, HE staining, and the thickness of dentin. Results: After 4 weeks, the hyperemia of experimental group reduced. After 6 weeks, X-ray could observe the dentin of experimental group was becoming thick and root apical foramen closed. After 6 weeks, newborn dentin in experimental group obviously increased in light microscope and odontoblasts arranged palisade. The sample in experimental group showed that after 2 weeks, dentin was becoming thick, and 4 weeks was more thick than it, continued to 6 weeks. The experimental group was better than the control group. Conclusion: It had potential clinical application value that the pulp cavity as the place of dental pulp stem cells implanting could promote pulp-dentin complex’s regeneration and repairing.

Key words: Dental pulp stem cell, Pulp-dentin complex, Regeneration, Repairing